Wastewater and Air Samples Collection and Pre‐Processing
1:55
Quantification of SARS‐CoV‐2 RNA by RT‐qPCR
2:46
Sequencing the Variants
7:37
Results: Detection and Quantification of SARS‐CoV‐2 RNA in Wastewater and Air Samples
8:31
Conclusion
Transcript
Wastewater-based epidemiology is currently used to complement COVID-19 control strategies worldwide by detecting viral RNA in wastewater samples and identifying potential outbreaks. This technique provides a population-wide snapshot of the prevale
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This protocol aims to quantify SARS-CoV-2 RNA in wastewater and air samples to be used for wastewater-based epidemiology studies and to assess the exposure risk to SARS-CoV-2 in indoor and outdoor aerosols. This protocol also describes a tiled amplicon long-template sequencing approach for SARS-CoV-2 whole genome characterization.