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2.0K Views
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07:12 min
June 30th, 2023
DOI :
10.3791/65056-v
Chapters
0:05
Introduction
1:02
Pollen Collection
1:40
In Vitro Pollen Germination Test
2:27
Pollination
3:02
Sampling, Fixation, and Preservation
3:55
Aniline Blue Staining
4:46
Fluorescence Microscopy
5:26
Results: Determining Pollen Compatibility and Incompatibility in Citrus Cultivars
6:16
Conclusion
Transcript
该技术可以有效地识别S基因型并准确识别柑橘的自身不相容性,为选择合适的传粉树提供必要的信息。这是一种在育种计划中选择合适的亲本树的省时、简单且可靠的技术。这种技术简单易行,第一次做实验需要耐心和细心。
S基因型鉴定和S特异性引物设计需要更多的关注和关注。演示该程序的将是我实验室的硕士研究生Yu Hu。通过将Majia pomelo,柑橘,花朵带到实验室开始花粉收集。
用镊子收集花药,并将它们放入含有滤纸的培养皿中。通过将含有花药的培养皿放入 28 摄氏度的烤箱中干燥
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Summary
该协议提供了一种快速确定柑橘品种中花粉相容性和不相容性的方法。
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