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07:39 min
February 3rd, 2023
DOI :
10.3791/65075-v
Chapters
0:04
Introduction
0:52
Setting Up of the High‐Resolution Respirometer
2:04
Measurement of Mitochondrial Membrane Potential Using Tetramethylrhodamine Methylester (TMRM)
2:54
Measurement of ATP Production Using Magnesium Green (MgG)
3:46
Measurement of Mitochondrial Production of ROSs Using Amplex UltraRed (AmR)
4:54
Measurement of Mitochondrial Respiration
6:19
Results: Characterization of Mitochondrial Physiology in Equine Skeletal Muscle
7:02
Conclusion
Transcript
这些协议允许研究人员超越简单地测量线粒体耗氧量,而是允许测量关键的线粒体最终产物,ATP和活性氧。该技术的主要优点是,研究人员可以通过直接比较耗氧率与最终产品生产率来测量线粒体效率。最终产品是ATP还是活性氧。
这些技术需要非常仔细地注意细节,特别是在避免气泡方面。无论是在呼吸室还是滴定注射器中,气泡都是您的敌人,因为它们会使测量变得不那么精确。获得骨骼肌活检后。
用无镁培养基填充呼吸计室并密封培养室。将仪器孵化温度设置为 38 摄氏度,以表示马骨骼肌的基础温度。并
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Summary
马具有非凡的有氧运动能力,使马骨骼肌成为研究马运动生理学和哺乳动物线粒体生理学的重要组织。本文介绍了全面评估马骨骼肌线粒体功能的技术。
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