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2.2K Views
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01:25 min
June 2nd, 2023
DOI :
10.3791/65206-v
Transcript
这种成像方法允许肝脂滴沉积的三维重建,并克服了经典组织学染色的主要缺点,这些缺点缩小了微观和大泡脂肪变性之间的成功区分。微泡性脂肪变性的评估是使用金标准组织学染色,由于天然脂滴的破坏和偶尔融合,两者和油红。pro-BODIPY为微泡肝脂肪变性评估的常规方案提供了一种替代方案,因为它具有正确的疏水性,可快速摄取和有效标记脂滴,不仅对环境极性不敏感,而且选择性极强。
这种成像方法与免疫化学技术相结合,具有更广泛的应用,可以同时检测其他亚细胞抗原,这些抗原可能推进脂滴的生物发生和动力
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