Research
Education
Sign In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Please note that all translations are automatically generated. Click here for the English version.
729 Views
•
00:50 min
August 4th, 2023
DOI :
10.3791/65264-v
Transcript
我们的实验室研究分枝杆菌、结核病和宿主免疫系统之间的相互作用,重点是细菌和巨噬细胞之间的初始相互作用。巨噬细胞中使用了多种基因破坏技术,包括RNAi技术和CRISPR技术。主要挑战之一是巨噬细胞中基因破坏的低效率。
该方案能够快速有效地对原代巨噬细胞进行遗传操作,而无需克隆和质粒构建。这些方法应该广泛适用于免疫学,使研究人员能够破坏原代巨噬细胞中的基因,以进行广泛的研究。
Sign in or start your free trial to access this content
Explore More Videos
From the series
Privacy
Terms of Use
Policies
Contact Us
Recommend to library
JoVE NEWSLETTERS
JoVE Journal
Methods Collections
JoVE Encyclopedia of Experiments
Archive
JoVE Core
JoVE Business
JoVE Science Education
JoVE Lab Manual
Faculty Resource Center
Authors
Librarians
Access
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved