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Please note that all translations are automatically generated. Click here for the English version.
1.3K Views
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02:35 min
January 19th, 2024
DOI :
10.3791/65544-v
Transcript
我们开发了一种高度灵敏的方法,使用蛋白质组富集微球从药物产品中检测那些低丰度的宿主细胞蛋白。这种方法有助于我们找到与药品相关的根本原因和风险。有多种方法可以提高HCP分析的检测限,非靶向方法包括但不限于蛋白A去除、有限消化、截留分子量、免疫沉淀。
靶向检测宿主细胞蛋白的方法,包括液相色谱法、多反应监测和非液相色谱平行反应监测。与这些HCP分析相关的主要挑战是抗体药物和宿主细胞蛋白之间的显着动态范围。因此,大多数方法旨在减少抗体的量,并在LCMS分析前富集HCP,以减小抗体肽和H
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