Name: Forfatter Spotlight: Effektive rotteklær for forbedret rottefiksering under elektroakupunktur
Uploaded: 2023-08-18T14:05:00
Description: Watch this Scientific Journal Video about Efficient Rat Clothing for Improved Rat Fixation During Electroacupuncture at JoVE.com

Forfatterne ønsker å uttrykke sin takknemlighet til Figdraw vitenskapelig forskning tegning plattform av Home for Researchers for å gi materialet som brukes til å lage figur 2 i dette papiret, med autorisert lisens-ID: URUUO5699e. Dette arbeidet har mottatt støtte fra tilskudd fra National Natural Science Foundation of China (81873380, 82074566), Natural Science Foundation of Hubei Province (2022CFCO43), og National Famous Traditional Chinese Medicine Heritage Studio Construction Project of the National Administration of Traditional Chinese Medicine ([2022] No.5, [2022] No.75).

Alle prosedyrer utført på rotter i denne studien fulgte National Standard for Laboratory Animal Welfare and Ethics Review Guidelines (2018) offisielt vedtatt av Folkerepublikken Kina og Veiledning for omsorg og bruk av forsøksdyr fra National Academies Press15. Denne protokollen består av tre hovedtrinn. For det første skreddersys og monteres rottejakkene basert på de individuelle kroppsformene til rotter med ulik vekt. Deretter utføres akupunkturmanipulasjon etter standardprosedyrer. Til s...

Rottejakker for å forbedre suspensjonsfiksering under elektroakupunktur på rotter

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	<li>Liu, Z., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effect+of+Electroacupuncture+on+Urinary+Leakage+Among+Women+With+Stress+Urinary+Incontinence:+A+Randomized+Clinical+Trial.">Effect of Electroacupuncture on Urinary Leakage Among Women With Stress Urinary Incontinence: A Randomized Clinical Trial.</a> The Journal of the American Medical Association. 317 (24), 2493-2501 (2017).</li><li>Tsuei, J. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Recent+developments+in+clinical+acupuncture.">Recent developments in clinical acupuncture.</a> The American Journal of Chinese Medicine. 11 (1-4), 150-158 (1983).</li><li>Liu, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Somatotopic+Organization+and+Intensity+Dependence+in+Driving+Distinct+NPY-Expressing+Sympathetic+Pathways+by+Electroacupuncture.">Somatotopic Organization and Intensity Dependence in Driving Distinct NPY-Expressing Sympathetic Pathways by Electroacupuncture.</a> Neuron. 108 (3), 436-450 (2020).</li><li>Liu, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+neuroanatomical+basis+for+electroacupuncture+to+drive+the+vagal-adrenal+axis.">A neuroanatomical basis for electroacupuncture to drive the vagal-adrenal axis.</a> Nature. 598 (7882), 641-645 (2021).</li><li>Li, X., Wei, W., Wang, Y., Wang, Q., Liu, Z. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Global+Trend+in+the+Research+and+Development+of+Acupuncture+Treatment+on+Parkinson's+Disease+From+2000+to+2021:+A+Bibliometric+Analysis.">Global Trend in the Research and Development of Acupuncture Treatment on Parkinson's Disease From 2000 to 2021: A Bibliometric Analysis.</a> Frontiers In Neurology. 13, 906317 (2022).</li><li>Jiang, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Application+Progress+of+fixation+methods+for+rodents+under+non+anesthesia+in+acupuncture+and+moxibustion+experiments+domestically+and+abroad.">Application Progress of fixation methods for rodents under non anesthesia in acupuncture and moxibustion experiments domestically and abroad.</a> Shanghai Journal of Acupuncture and Moxibustion. 40 (12), 1509-1514 (2021).</li><li>Jiang, H. Z., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Fixation+method+in+the+study+of+acupuncture+intervention+on+rats.">Fixation method in the study of acupuncture intervention on rats.</a> Journal of Shanxi College of Traditional Chinese Medicine. 20 (3), 169-171 (2019).</li><li>Wang, Y., Ning, Y., Wang, P. Y., Yang, Y. Q. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Method+of+Placing+a+Rat+on+an+Elevated+Platform+for+Acupuncture.">Method of Placing a Rat on an Elevated Platform for Acupuncture.</a> Shanghai Journal of Acupuncture and Moxibustion. 31 (5), 289-292 (2012).</li><li>Wang, F., Xiong, J., Zeng, F., He, Z. X. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+fixation+frame+for+acupuncture+and+moxibustion+of+Shu-Mu+acupoint+combination+in+rats.">A fixation frame for acupuncture and moxibustion of Shu-Mu acupoint combination in rats.</a> Acupuncture Research. 47 (10), 938-940 (2022).</li><li>Tu, S., P, C., Y, Y. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Fixation+method+for+needle+warming+moxibustion+treatment+on+swinging+rats+bound+with+coat.">Fixation method for needle warming moxibustion treatment on swinging rats bound with coat.</a> Chinese Journal of Comparative Medicine. 31 (5), 128-132 (2021).</li><li>Redfors, B., Shao, Y., Omerovic, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Influence+of+anesthetic+agent,+depth+of+anesthesia+and+body+temperature+on+cardiovascular+functional+parameters+in+the+rat.">Influence of anesthetic agent, depth of anesthesia and body temperature on cardiovascular functional parameters in the rat.</a> Laboratory Animals. 48 (1), 6-14 (2014).</li><li>Kiyatkin, E. A., Brown, P. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Brain+and+body+temperature+homeostasis+during+sodium+pentobarbital+anesthesia+with+and+without+body+warming+in+rats.">Brain and body temperature homeostasis during sodium pentobarbital anesthesia with and without body warming in rats.</a> Physiology &amp; Behavior. 84 (4), 563-570 (2005).</li><li>He, C., Zhang, C., Zhou, S., Fan, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Using+old+meshy+cage+cover+to+make+a+rat+fixating+device+for+acupuncture+study.">Using old meshy cage cover to make a rat fixating device for acupuncture study.</a> Acupuncture Research. 46 (4), 348-350 (2021).</li><li>Li, Y., Lin, N., Huang, Y., Xue, X., Liu, Q. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+Newly-made+Rat+Holder+for+Convenient+Acupuncture+Needle+Insertion.">A Newly-made Rat Holder for Convenient Acupuncture Needle Insertion.</a> Acupuncture Research. 41 (5), 466-468 (2016).</li><li>Bielitzki, J. T., Barbee, R. W., Garber, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Guide for the care and use of laboratory animals. 85 (3), 963-965 (2011).</li><li>Zheng, X., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Electroacupuncture+ameliorates+beta-amyloid+pathology+and+cognitive+impairment+in+Alzheimer+disease+via+a+novel+mechanism+involving+activation+of+TFEB+(transcription+factor+EB).">Electroacupuncture ameliorates beta-amyloid pathology and cognitive impairment in Alzheimer disease via a novel mechanism involving activation of TFEB (transcription factor EB).</a> Autophagy. 17 (11), 3833-3847 (2021).</li><li>Su, X., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effectiveness+and+Safety+of+Acupuncture+for+Vascular+Cognitive+Impairment:+A+Systematic+Review+and+Meta-Analysis.">Effectiveness and Safety of Acupuncture for Vascular Cognitive Impairment: A Systematic Review and Meta-Analysis.</a> Frontiers In Aging Neuroscience. 13, 692508 (2021).</li><li>Wang, Q., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Efficacy+of+electroacupuncture+pretreatment+for+myocardial+injury+in+patients+undergoing+percutaneous+coronary+intervention:+A+randomized+clinical+trial+with+a+2-year+follow-up.">Efficacy of electroacupuncture pretreatment for myocardial injury in patients undergoing percutaneous coronary intervention: A randomized clinical trial with a 2-year follow-up.</a> International Journal of Cardiology. 194, 28-35 (2015).</li><li>Zhang, R., Lao, L., Ren, K., Berman, B. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mechanisms+of+acupuncture-electroacupuncture+on+persistent+pain.">Mechanisms of acupuncture-electroacupuncture on persistent pain.</a> Anesthesiology. 120 (2), 482-503 (2014).</li></ol>

Elektroakupunktur har vist seg å ha god terapeutisk effekt på ulike sykdommer som kognitiv svikt 16,17, myokardskade18 og betennelsessmerter19. For å forstå virkningsmekanismen er det utført dyreforsøk primært med rotter. Fiksering av dyrene under prosedyrene er avgjørende for nøyaktige resultater. Mens mange studier har administrert elektroakupunktur etter dyrebedøvelse, kan denne praksisen hindre hjernen...

Akupunktur er en tradisjonell terapi preget av nåling på bestemte akupunkter eller andre stillinger for å forebygge og behandle sykdommer. Elektroakupunktur kombinerer filiforme nåler med elektrisk stimulering1, noe som muliggjør presis kontroll over mengden stimulering ved å justere relevante parametere, fremme standardisering av akupunkturbehandling2. I de senere år har forskning på mekanismene for elektroakupunktur fortsatt å utvide og utdype3,4, noe som har ført til en årlig økning i studier som fokuserer på mekanismene for elektroakupunktur5.
I grunnforskning på elektroakupunktur er rotter de mest brukte dyrene. Under elektroakupunkturbehandling må mottakeren forbli stille for å sikre en jevn prosedyre. I motsetning til i kliniske forsøk med mennesker, kan små dyr som brukes i dyreforsøk ikke følge kommandoer bevisst under 15-30 min lange elektroakupunkturprosedyrer. Dette gir forskere en primær utfordring med å oppnå stabil og pålitelig fiksering. Feil fiksering kan føre til negative følelser som angst, depresjon og frykt hos dyr6, noe som ikke bare øker risikoen for skade for rotter og sjansen for å bli bitt for eksperimentelt personell, men også påvirker stabiliteten og overtalelsesevnen til forskningsresultater.
I tidligere studier har forskere ofte brukt anestesifiksering7, høy plattformfiksering8, bindingsfiksering 6,9 eller rottebeleggfiksering10 før akupunktur eller elektroakupunkturoperasjoner. Men gitt effekten av anestesi på kroppen11 og hjernefunksjon12, og med tanke på at akupunktur utføres på bevisste pasienter i kliniske tilfeller, kan anestesi til en viss grad påvirke forskningsresultatene. Derfor, sammenlignet med anestesifiksering, er komfortabel fiksering av rotter i våken tilstand et bedre alternativ.
I ikke-bedøvet tilstand har imidlertid forskjellige fikseringsmetoder også sine respektive begrensninger, inkludert begrenset akupunkteksponering på grunn av dekning av rottebelegget på rotter 10, nødvendigheten av minst to personer til å utføre operasjonen13, ikke-justerbar fikseringsstørrelse14 eller den stressede tilstanden til rotter i en tvungen stilling6. For å løse disse tekniske problemene designet vi en rottejakke og et festestativ som er egnet for akupunkturmanipulering i de fleste deler av rottekroppen. Dette kan hjelpe forskere effektivt å utføre akupunkturprosedyrer med rotter.
Begrunnelsen for denne protokollen er som følger: For det første utnytter det lukkede hodeplagget på rottejakken rottenes naturlige tilbøyelighet til mørke og graving, og begrenser dermed deres aktivitetsområde. For det andre gir bindingstauene nær leddene i rotternes lemmer fysisk kompresjonsbegrensning, og pålegger visse begrensninger på bevegelsene deres. Imidlertid er rotter fortsatt i stand til å hoppe og bevege seg horisontalt selv mens de har på seg jakken. Til slutt, siden rotter ikke kan berøre bakken uten et fast kraftpunkt når de er suspendert, la vi til en opphengsfikseringsenhet til rottejakken, noe som ytterligere begrenset bevegelsene deres og sikret nåleretensjon. Sammensetningen av fikseringsanordningen er vist i figur 1.
<img alt="Figure 1" class="xfigimg" src="/files/ftp_upload/65652/65652fig01.jpg"> Figur 1: Skjematisk diagram over rottejakke og fjæringsfiksering. (A) Det generelle utseendet til rottejakken i flat visning. (B) Tredimensjonalt bilde av rottejakken. (C) Spesialtilpasset rottejakke med snitt nær akupunktene på hodet og baksiden. (D) Illustrasjon av flere rotter som henger samtidig på den forhøyede stativet. 1: Stoff samlet visning; 2: Forbenet utløp; 3: Bakre lem utløp; 4: Binde tau; 5: Hulrom dannet etter suturering; 6: Snitt for å eksponere hodet akupunkter; 7: Snitt for å eksponere rygg og midje akupunkter. 8: Suturposisjon. <a href="https://www.jove.com/files/ftp_upload/65652/65652fig01large.jpg" target="_blank">Klikk her for å se en større versjon av denne figuren.</a>

<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>75% alcohol</td>
			<td>Shandong Lircon medical technology co., ltd</td>
			<td>2002-0059</td>
			<td>Disinfection</td>
		</tr>
		<tr>
			<td>acupuncture needles</td>
			<td>Beijing Luo ya shan chuan medical equipment co., Ltd.</td>
			<td></td>
			<td>needling</td>
		</tr>
		<tr>
			<td>Adult Sprague-Dawley (SD) rats</td>
			<td>Liaoning Changsheng biotechnology co., Ltd.</td>
			<td>SCXK 2020-0001</td>
			<td>The recipient&#160; subjected to fixation</td>
		</tr>
		<tr>
			<td>an elevated fixation rack&#160;</td>
			<td>Taizhou shiai instruments and equipments for scientific and teaching research&#160; Co., ltd</td>
			<td>/</td>
			<td>Fix rats</td>
		</tr>
		<tr>
			<td>Hans electronic acupuncture apparatus</td>
			<td>Beijing Huayun Ante Science and Technology Co. Ltd.&#160;</td>
			<td>HANS-200A</td>
			<td>electroacupuncture</td>
		</tr>
		<tr>
			<td>Medical cotton ball</td>
			<td>Jiangsu Misawa Medical Supplies Co., Ltd.</td>
			<td>20172142357</td>
			<td>Disinfection</td>
		</tr>
		<tr>
			<td>Pet electric clipper</td>
			<td>Fenghua Xikou Lubao Knives &#38; Scissors Electrical Appliance Factory</td>
			<td>/</td>
			<td>Shave the hair of rats</td>
		</tr>
		<tr>
			<td>Tweezers</td>
			<td>Qingdao Schultz Biotechnology Co., ltd</td>
			<td></td>
			<td>Pinch cotton balls</td>
		</tr>
	</tbody>
</table>

improved suspension fixation using rat jacket in rat acupuncture experiments

I grunnleggende eksperimentell akupunkturforskning brukes rotter ofte som laboratoriedyr. Det er imidlertid vanskelig for dem å opprettholde en fast holdning. Under elektroakupunkturprosedyrer er riktig immobilisering av rotter viktig. Ulike metoder for rottefiksering brukes for tiden, inkludert anestesifiksering, fiksering av høy plattform, bindingsfiksering og fiksering med et selvfremstillet rottebelegg. Disse metodene har imidlertid sine begrensninger, noe som til en viss grad kan påvirke eksperimentets effektivitet og brukbarhet. Denne protokollen introduserer en metode for å suspendere og fikse rotter ved hjelp av rotteklær. For det første er rotter kledd med rottejakker som matcher kroppsfasongen, og utnytter deres preferanse for mørke og graving. Nåleoperasjonen kan deretter utføres etter at rottene har brukt rotteklær. Når de er suspendert, er rottene relativt stille, da deres lemmer ikke kan bevege seg. Denne fikseringsmetoden gir ikke bare økonomiske og brukervennlige fordeler, men sikrer også en stabil og pålitelig fiksering av rottene i en komfortabelt avslappet stilling. Det minimerer også effektivt tidsforbruk, eksperimentell plass og arbeidskraftressurser. I tillegg tillater denne metoden eksponering av de fleste akupunkturer som brukes til akupunktur hos rotter. Denne artikkelen konsentrerer seg primært om enhetens sammensetning, som omfatter en spesialdesignet rottejakke, et forhøyet fikseringsstativ og deres tilkoblingsstrukturer. I tillegg vil et illustrerende eksempel bli presentert for å demonstrere anvendelsen av rotteklærbasert suspensjonsfikseringsmetode i rotteelektroakupunkturprosedyrer.

Acupuncture is a traditional therapy characterized by needling at specific acupoints or other positions to prevent and treat diseases. Electroacupuncture combines filiform needles with electrical stimulation1, which allows for precise control over the amount of stimulation by adjusting relevant parameters, promoting the standardization of acupuncture treatment2. In recent years, research on the mechanisms of electroacupuncture has continued to expand and deepen3,4, which has led to an annual increase in studies focusing on the mechanisms of electroacupuncture5.
In basic research on electroacupuncture, rats are the most commonly used animals. During electroacupuncture treatment, the recipient must remain still to ensure a smooth procedure. Unlike in clinical trials with human subjects, small animals used in animal experiments cannot follow commands consciously during 15-30 min lengthy electroacupuncture procedures. This presents researchers with a primary challenge of achieving stable and reliable fixation. Improper fixation can lead to negative emotions such as anxiety, depression, and fear in animals6, which not only increases the risk of injury for rats and the chance of being bitten for the experimental personnel but also affects the stability and persuasiveness of research results.
In previous studies, researchers have often used anesthesia fixation7, high platform fixation8, binding fixation6,9, or rat coat fixation10 before acupuncture or electroacupuncture operations. However, given the effects of anesthesia on the body11 and brain function12, and considering that acupuncture is performed on conscious patients in clinical cases, anesthesia may impact research results to a certain extent. Therefore, compared with anesthesia fixation, comfortable fixation of rats in an awake state is a better option.
However, in the non-anesthetized state, various fixation methods also possess their respective limitations, including restricted acupoint exposure due to coverage of the rat coat on rats10, the necessity of at least two individuals to perform the operation13, non-adjustable fixator size14, or the stressed state of rats in a forced position6. To address these technical issues, we designed a rat jacket and a fixation rack suitable for acupuncture manipulation in most parts of the rat body. This can help researchers efficiently carry out acupuncture procedures with rats.
The rationale for this protocol is as follows: firstly, the closed headgear on the rat&#39;s jacket takes advantage of the rats&#39; natural inclination for darkness and burrowing, thus restricting their range of activities. Secondly, the binding ropes near the joints of the rats&#39; limbs provide physical compression restraint, imposing certain limitations on their movements. However, rats are still able to jump and move horizontally even while wearing the jacket. Lastly, since rats cannot touch the ground without a fixed force point when suspended, we added a suspension fixation device to the rat jacket, further restricting their movements and ensuring needle retention. The composition of the fixation device is shown in Figure 1.
<img alt="Figure 1" class="xfigimg" src="/files/ftp_upload/65652/65652fig01.jpg" /> 
Figure 1: Schematic diagram of the rat jacket and suspension fixation. (A) The general appearance of the rat jacket in a flat view. (B) Three-dimensional view of the rat jacket. (C) Specially tailored rat jacket with an incision near the acupoints on the head and back waist. (D) Illustration of multiple rats simultaneously suspended on the elevated rack. 1: Fabric overall view; 2: Forelimb outlet; 3: Hind limb outlet; 4: Tying rope; 5: Cavity formed after suturing; 6: Incision for exposing head acupoints; 7: Incision for exposing back and waist acupoints. 8: Suture position. <a href="https://www.jove.com/files/ftp_upload/65652/65652fig01large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>

Forfatter Spotlight: Effektive rotteklær for forbedret rottefiksering under elektroakupunktur 

Forbedret fjæringsfiksering ved hjelp av rottejakke i rotteakupunktureksperimenter

We developed an improved method for suspending and fixing rats with rat clothes in an acupuncture experiment. This protocol aims to provide detailed descriptions of its procedural steps, including important precautions, offering a reference for researchers conducting fundamental experiments in the field of acupuncture and moxibustion. In previously reported studies, researchers have often used anesthesia fixation, high platform fixation, binding fixation, or various other self-made fixators while performing acupuncture or electroacupuncture operations on rats.Given the side effects of anesthesia on the body and the real clinical conditions, fixation in an awakened stage offers the better alternative. However, in the non-anesthetized stage, the mentioned fixations also possess limitations such as restricted acupoint exposure, requirement of multiple labels, non-adjustable fixator size, or forced posture. This method utilizes the rat's preference for burrowing to create rat jackets that fit their body shape.It offers several advantages over other techniques. It is cost-effective, user-friendly, manpower-and space-saving, accommodates rats of varying weights, and can also be used for needle operation on most acupoints. In subsequent studies, we will focus on investigating the impact of the restraints and suspensions of rat clothing on physiological indicators and behavioral aspects related to emotional cognition in rats.This will enable us to quantitatively assess its potential influence on research and refine the fixation method.

I dette forsøket ble totalt 12 rotter valgt for elektroakupunkturbehandling, og deres reaksjoner under fikseringsmetoden ble observert (som vist i tabell 2). Blant de 12 rottene viste bare 1 rotte intens kamp i løpet av 15 min elektroakupunkturnålretensjonsperioden. Som et resultat av sine kraftige bevegelser kom nålen av midtveis, og utgjorde 8,33% av det totale antall rotter. Dette resultatet antyder at fikseringsmetoden som brukes generelt demonstrerer en betydelig grad av stabilitet og pålitelig...

Watch this Scientific Journal Video about Efficient Rat Clothing for Improved Rat Fixation During Electroacupuncture at JoVE.com

Her presenterer vi en forbedret suspensjonsfikseringsmetode ved hjelp av en rottejakke for effektivt å immobilisere rotter under akupunktureksperimenter. Videre belyses strukturen og anvendelsen av dette fikseringsapparatet, samt instruksjoner og evaluering for bruk i elektroakupunkturbehandling.

In basic experimental acupuncture research, rats are commonly used as laboratory animals. However, it is difficult for them to maintain a fixed posture. ...

Vi utviklet en forbedret metode for å suspendere og fikse rotter med rotteklær i et akupunktureksperiment. Denne protokollen tar sikte på å gi detaljerte beskrivelser av sine prosessuelle trinn, inkludert viktige forholdsregler, og tilbyr en referanse for forskere som utfører grunnleggende eksperimenter innen akupunktur og moxibustion. I tidligere rapporterte studier har forskere ofte brukt anestesifiksering, høy plattformfiksering, bindende fiksering eller forskjellige andre selvlagde fikseringsapparater mens de utfører akupunktur- eller elektroakupunkturoperasjoner på rotter.Gitt bivirkningene av anestesi på kroppen og de virkelige kliniske forholdene, gir fiksering i et våknet stadium det bedre alternativet. Imidlertid, i det ikke-bedøvede stadiet, har de nevnte fikseringene også begrensninger som begrenset akupunkteksponering, krav om flere etiketter, ikke-justerbar fiksatorstørrelse eller tvungen holdning. Denne metoden utnytter rottens preferanse for å grave for å lage rottejakker som passer til kroppsfasongen.Det gir flere fordeler i forhold til andre teknikker. Det er kostnadseffektivt, brukervennlig, arbeidskraft- og plassbesparende, har plass til rotter med varierende vekt, og kan også brukes til nåleoperasjon på de fleste akupunkter. I etterfølgende studier vil vi fokusere på å undersøke virkningen av begrensninger og suspensjoner av rotteklær på fysiologiske indikatorer og atferdsaspekter relatert til emosjonell kognisjon hos rotter.Dette vil gjøre det mulig for oss å kvantitativt vurdere dens potensielle innflytelse på forskning og avgrense fikseringsmetoden.

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JoVE Journal

Medicine

Improved Suspension Fixation Using Rat Jacket in Rat Acupuncture Experiments

In basic experimental acupuncture research, rats are commonly used as laboratory animals. However, it is difficult for them to maintain a fixed posture. During electroacupuncture procedures, proper immobilization of rats is essential. Various methods of rat fixation are currently used, including anesthesia fixation, high-platform fixation, binding fixation, and fixation with a self-made rat coat. However, these methods have their limitations, which may affect the efficiency and operability of the experiment to some extent. This protocol introduces a method of suspending and fixing rats using rat clothes. Firstly, rats are clothed with rat jackets that match their body shape, taking advantage of their preference for darkness and burrowing. The needling operation can then be carried out after the rats have worn rat clothes. When suspended, the rats are relatively still, as their limbs cannot move. This fixation method offers not only economical and user-friendly benefits but also ensures a stable and reliable fixation of the rats in a comfortably relaxed position. It also effectively minimizes time consumption, experimental space, and manpower resources. Additionally, this method allows for the exposure of most acupoints used for acupuncture in rats. This article primarily concentrates on the device's composition, encompassing a specially designed rat jacket, an elevated fixation rack, and their connecting structures. Additionally, an illustrative example will be presented to demonstrate the application of the rat clothing-based suspension fixation method in rat electroacupuncture procedures.

Here, we present an improved suspension fixation method using a rat jacket to effectively immobilize rats during acupuncture experiments. Furthermore, the structure and application of this fixation apparatus, as well as instructions and evaluation for its utilization in electroacupuncture therapy, is elucidated.

Securing the Rat in the Rat Jacket for Electroacupuncture

Wear a pair of gloves before proceeding to fit and secure the rat in the custom made rat jacket. Then using one hand hold the rat jacket's middle and back portion on the table. Using the other hand, gently hold the rat by its tail to pick it up from the feeding cage and place it into the jacket as the rat moves forward into the jacket and its head reaches the headgear point.Using both hands, carefully pull out its left and right upper limbs from the holes in the jacket. Securely wrap the tying ropes from the opening end of the jacket around the rat's trunk, and tie them tightly at the opposite end to adequately restrain its upper limbs. Tie the rope loose enough to prevent hypoxia or local limb ischemia, and tight enough to prevent the rat's escape.In a similar manner, secure its lower limbs.

Sikre rotta i rottejakken for elektroakupunktur

Performing Electroacupuncture on Rat Immobilized Using Rat Jacket

Before beginning the procedure of electropuncture on the rat, dress the rat in the custom made rat jacket. Then disinfect the local skin of acupoints using alcohol swabs. After locating the acupoint accurately, insert the sterile disposable acupuncture needle into the spot at the appropriate depth.Gently suspend the animal from the horizontal iron rod with the help of the S-shaped hooks attached to the rat jacket. Wrap the electrode wire around the horizontal iron rod before securely attaching the small clip at the end of the electrode wire to the needle handle. Then after switching on the power, select the appropriate frequency, current, and time.Once the parameters are adjusted, commence the electroacupuncture treatment process. Using the S-shaped hooks, suspend the rats in the same group together on the iron rod for simultaneous electroacupuncture. When the treatment is over, remove the acupuncture needle along with the clamp from the animal.Then carefully take the needle out of the clamp and put it in the box. After removing the animal from the suspension hooks, loosen the straps, lift the head cover of the rat's jacket and position the rat above the cage. The rat will then be free to release itself from the jacket and enter the cage independently.While performing a 15 minute electroacupuncture experiment on 12 rats only one rat exhibited intense struggle during the needle retention period resulting in needle drop. While other rats maintained proper needle fixation throughout the treatment. When compared to the other fixation methods reported in the literature, the demonstrated method exhibited the combined benefits of stability and secure fixation, efficiency in terms of time and number of personnel required, affordability, as well as relative comfort for the rats.Additionally, in terms of the exposed acupoint range, the specially designed rat jackets in this protocol met the requirements for exposing most acupoints on the body.