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01:42 min
September 15th, 2023
DOI :
10.3791/65687-v
Transcript
内耳被包裹在颞骨中,深深地扎在身体最坚硬的骨头中,对调查构成了挑战。原代毛细胞的培养对于研究耳蜗毛细胞是必不可少的。本研究旨在发现一种分离和培养小鼠毛细胞的方法。
我们演示了从天然小鼠中分离耳蜗器官和不对称分离丰富的毛细胞以进行体外研究的步骤。使用免疫荧光染色确认培养细胞的性质。从耳蜗器官中分离活细胞,并立即指导它们,成为广泛研究的宝贵工具,主要培养方法有三种,这三种系统都是三种。
该方法可以增强我们对体内培养毛细胞生物学特性的理解,并证明耳蜗毛细胞培养的效率,为进
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