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Please note that all translations are automatically generated. Click here for the English version.
769 Views
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01:28 min
August 18th, 2023
DOI :
10.3791/65785-v
Transcript
我们的实验室旨在研究肝癌的分子发病机制,并确定药物开发的新治疗靶点。我们想利用新的疾病模型(如患者来源的类器官)来回答癌症如何启动、转移和进展为药物耐受性。目前的实验挑战主要体现在两个方面。
首先,缺乏一套完整的肝细胞癌类器官构建方案。其次,培养的HCC类器官的尺寸太小,无法进行后续的实验处理。在该协议中,我们提供了优化的类器官培养和随后的转移和冷冻程序的详细信息,以及在所有程序中应采取的预防措施。
我们优化了类器官培养基的组成,使其具有接近原始组织特征的类器官。我
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