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	<li>Mirin, A. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Gender+disparity+in+the+funding+of+diseases+by+the+U.S.">Gender disparity in the funding of diseases by the U.S.</a> J Womens Health. 30 (7), 956-963 (2021).</li><li>MacLennan, A. H., Taylor, A. W., Wilson, D. H., Wilson, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+prevalence+of+pelvic+floor+disorders+and+their+relationship+to+gender,+age,+parity,+and+mode+of+delivery.">The prevalence of pelvic floor disorders and their relationship to gender, age, parity, and mode of delivery.</a> BJOG. 107 (12), 1460-1470 (2000).</li><li>Ruiz-Zapata, A. M., Kerkhof, M. H., Zandieh-Doulabi, B., Br&#246;lmann, H. A. M., Smit, T. H., Helder, M. N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Fibroblasts+from+women+with+pelvic+organ+prolapse+show+differential+mechanoresponses+depending+on+surface+substrates.">Fibroblasts from women with pelvic organ prolapse show differential mechanoresponses depending on surface substrates.</a> Int Urogynecol J Pelvic Floor Dysfunct. 24 (9), 1567-1575 (2013).</li><li>Kaur, G., Dufour, J. 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<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>Amphotericin B</td>
			<td>Bio Techne</td>
			<td>B23192</td>
			<td></td>
		</tr>
		<tr>
			<td>Cell strainer (100 &#956;m)</td>
			<td>ThermoFisher Scientific</td>
			<td>22363549</td>
			<td></td>
		</tr>
		<tr>
			<td>Conical Centrifuge Tubes (15 mL)</td>
			<td>Fisher Scientific&#160;</td>
			<td>14-959-53A</td>
			<td></td>
		</tr>
		<tr>
			<td>DMEM/F12</td>
			<td>ThermoFisher Scientific</td>
			<td>11320033</td>
			<td></td>
		</tr>
		<tr>
			<td>Feather Disposable Scalpel No. 15</td>
			<td>Socorex</td>
			<td>FB.15</td>
			<td></td>
		</tr>
		<tr>
			<td>Fetal Bovine Serum</td>
			<td>Sigma Aldrich</td>
			<td>NC1983075</td>
			<td></td>
		</tr>
		<tr>
			<td>High Clarity Conical Centrifuge Tubes (50 mL)</td>
			<td>Fisher Scientific&#160;</td>
			<td>14-432-22</td>
			<td></td>
		</tr>
		<tr>
			<td>HulaMixer Sample Mixer</td>
			<td>ThermoFisher Scientific</td>
			<td>15920D</td>
			<td></td>
		</tr>
		<tr>
			<td>Human Fibronectin DuoSet ELISA</td>
			<td>R&#38;D Systems</td>
			<td>DY1918-05</td>
			<td></td>
		</tr>
		<tr>
			<td>Human Pro-Collagen I alpha 1 DuoSet ELISA</td>
			<td>R&#38;D Systems</td>
			<td>DY6220-05</td>
			<td></td>
		</tr>
		<tr>
			<td>Liberase Research Grade</td>
			<td>Sigma Aldrich</td>
			<td>05 401 119 001</td>
			<td></td>
		</tr>
		<tr>
			<td>Penicillin/Streptomycin (5000U/ml)</td>
			<td>ThermoFisher Scientific</td>
			<td>15070063</td>
			<td></td>
		</tr>
		<tr>
			<td>Petri dishes, polystyrene (100 mm x 20 mm)</td>
			<td>Millipore Sigma</td>
			<td>P5606</td>
			<td></td>
		</tr>
		<tr>
			<td>Serologic pipettes (10 mL)</td>
			<td>ThermoFischer Scientific</td>
			<td>170356N</td>
			<td></td>
		</tr>
		<tr>
			<td>Sterile syringes (5 mL)</td>
			<td>Fischer Scientific</td>
			<td>14-955-458</td>
			<td></td>
		</tr>
	</tbody>
</table>

tissue processing and isolation of primary fibroblasts from the human vagina

Processamento de tecidos e isolamento de fibroblastos primários da vagina humana

Pelvic organ prolapse is a disorder that seriously impacts the quality of life of women. It occurs when muscles and ligaments weaken and cause pelvic ...

tissue-processing-isolation-primary-fibroblasts-from-human

Research

JoVE Journal

Biology

Tissue Processing and Isolation of Primary Fibroblasts from the Human Vagina

143 Views.  Massachusetts General Hospital. Research into disorders that primarily affect people of female gender is understudied and underfunded. Pelvic organ prolapse is the disorder strongly associated with female gender. It occurs when muscles and ligaments weaken and cause pelvic organs to drop lower in the pelvis, creating a bulge in the vagina.Little is known about cellular interactions in the context of this pathophysiology, nor how tissue level factors impact success of surgical interventions. Isolation of primary fibro...