Name: लेखक स्पॉटलाइट: Rhodiola crenulata के लिए पहचान के तरीके विकसित करना और इसके संसाधन वितरण और औषधीय प्रभावों की जांच करना
Uploaded: 2023-10-27T13:20:00
Description: Watch this Scientific Journal Video about Field Collection and Laboratory Routine Identification of Rhodiola crenulata at JoVE.com

इस काम को चीन के राष्ट्रीय प्राकृतिक विज्ञान फाउंडेशन (81973569, 82274207 और 82104533), टीसीएम (XKTD2022013) के चेंगदू विश्वविद्यालय के ज़िंगलिन स्कॉलर रिसर्च प्रमोशन प्रोजेक्ट और निंग्ज़िया के प्रमुख अनुसंधान और विकास कार्यक्रम (2023BEG02012) द्वारा समर्थित किया गया था।

Rhodiola crenulata Zhuoda हिमपात पर्वत, Ganzi काउंटी, Ganzi तिब्बती स्वायत्त प्रान्त, सिचुआन प्रांत, चीन (N 31.44570 °, ई 99.96086 °, 4892 मीटर) से एकत्र किया जाता है। चेंगदू यूनिवर्सिटी ऑफ ट्रेडिशनल चाइनीज मेडिसिन के स्कूल ऑफ एथनिक मेडिसि...

सूक्ष्म लक्षण वर्णन और Rhodiola crenulate के टीएलसी आधारित पहचान

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दुनिया में रोडियोला पौधों की 90 से अधिक प्रजातियां हैं, और चीन में सभी प्रजातियों की 60% से अधिक पाई जाती हैं, रोडियोला क्रेनुलाटा, रोडियोला रसिया, रोडियोलस अचलिनेंसिस और रोडियोला एल्गिडा<sup class="xref...

हर्बल दवा का चीन में एक लंबा इतिहास और समृद्ध अनुप्रयोग अनुभव है, और यह शेनॉन्ग के हर्बल क्लासिक1 में पहली व्यवस्थित रिकॉर्डिंग थी। मलेरिया पर लागू आर्टेमिसिनिन की खोज ने हर्बल दवा के विकास को एक नए चरण1 में बढ़ावा दिया। हर्बल दवा के सटीक तंत्र को उजागर करने के लिए आधुनिक वैज्ञानिक प्रौद्योगिकी का उपयोग हर्बल दवा के लिए उपयोग दर और मांग बढ़ जाती है, इसके लिए एक नया अंतरराष्ट्रीय बाजार खोलने 2,3,4. हालांकि, इससे नकारात्मक प्रभावों की एक श्रृंखला हुई है। गैर-पेशेवरों को हर्बल दवा की विशेषताओं की अस्पष्ट समझ है, जो हर्बल दवा के उपयोग को एक बड़ा सुरक्षा जोखिम का सामना करतीहै 5.
Rhodiola crenulata, Rhodiola प्रजातियों के पौधों में से एक, मुख्य रूप से तिब्बत, उत्तर पश्चिम युन्नान और चीन के पश्चिमी सिचुआन (चित्रा 1)6,7में वितरित किया जाता है. Rhodiola crenulata में "स्फूर्तिदायक क्यूई और रक्त परिसंचरण को बढ़ावा देने, नाड़ी को साफ करने और अस्थमा को शांत करने"8,9,10,11के कार्य के माध्यम से हाइपोक्सिक से संबंधित बीमारियों के उपचार के लिए सैलिड्रोसाइड, टायरोसोल, गैलिक एसिड और अन्य यौगिक शामिल हैं। फील्ड जांच से पता चलता है कि रोडियोला क्रेनुलाटा अल्पाइन ताल क्षेत्रों, गली ढलानों और रॉक दरारों में 4,000-5,600 मीटर की ऊंचाई पर पाया जा सकता है। इसका बढ़ता वातावरण ठंडा है, धूप और तीव्र विकिरण से भरा है, और यह अल्पाइन घास के मैदान के पारिस्थितिकी तंत्र से संबंधित है। Rhodiola crenulata विकास इलाके के अनुसार lamellar और बिंदु की तरह आबादी में वितरित किया जा सकता है, और जीन प्रवाह पार परागण के माध्यम से बाहर किया जा सकता है.
जीनस Rhodiola के पराग गर्भपात, अवैध खुदाई, और पतित पारिस्थितिक पर्यावरण Rhodiola crenulata एक लुप्तप्राय प्रजाति 6,12 बनाने. Rhodiola crenulata के उच्च औषधीय मूल्य को देखते हुए, नकली उत्पादों बाजार में प्रवाह की उम्मीद कर रहे हैं. यह लेख Rhodiola crenulata के निवास स्थान और कुछ सुविधाजनक प्रयोगशाला पहचान विधियों प्रस्तुत करता है. सबसे पहले, हम Rhodiola crenulata और इसके औषधीय गुणों के विकास पर्यावरण मनाया. दूसरे, औषधीय पाउडर की माइक्रोस्ट्रक्चर माइक्रोस्कोप द्वारा देखी गई थी। अंतिम चरण मुख्य बिंदु है। Rhodiola crenulata के प्रतिनिधि घटकों को अलग किया गया और एक निश्चित पदार्थ में इन घटकों के विभिन्न सोखना या विघटन गुणों के अनुसार पहचाना गया. औषधीय पौधों के डीएनए आधारित प्रमाणीकरण या चयापचय विश्लेषण विधियों जटिल और महंगा13 कर रहे हैं. ये बुनियादी, सुविधाजनक, और किफायती तरीके जल्दी से Rhodiola crenulata की पहचान कर सकते हैं.

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			<td>Hangzhou Xingrun sieve factory</td>
			<td>572423281330</td>
			<td></td>
		</tr>
		<tr>
			<td>Pipette</td>
			<td>Changde BKMAM Biotechnology Co., Ltd</td>
			<td>120302008</td>
			<td></td>
		</tr>
		<tr>
			<td>Salidroside</td>
			<td>Chengdu Herbpurify Co., Ltd</td>
			<td>H-040</td>
			<td></td>
		</tr>
		<tr>
			<td>Saturate tank&#160;</td>
			<td>Yancheng Liegu Technology Co., Ltd</td>
			<td>10*20 P-1</td>
			<td></td>
		</tr>
		<tr>
			<td>Silica gel plate</td>
			<td>Yantai Jiangyou Silica Gel Development&#160; Co., Ltd</td>
			<td>HSG20211227</td>
			<td></td>
		</tr>
		<tr>
			<td>Trichloromethane</td>
			<td>Chengdu Kelong Chemical Co., Ltd</td>
			<td>20221013-1</td>
			<td></td>
		</tr>
		<tr>
			<td>Tweezer&#160;</td>
			<td>Changde BKMAM Biotechnology Co., Ltd</td>
			<td>130302027</td>
			<td></td>
		</tr>
		<tr>
			<td>Tyrosol</td>
			<td>Chengdu Herbpurify Co., Ltd</td>
			<td>L-042</td>
			<td></td>
		</tr>
		<tr>
			<td>Ultrasound equipment</td>
			<td>Ningbo Xinyi Ultrasonic Equipment Co., Ltd</td>
			<td>SB-8200DTS</td>
			<td></td>
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			<td>Volumetric pipet</td>
			<td>Changde BKMAM Biotechnology Co., Ltd</td>
			<td>120301006</td>
			<td></td>
		</tr>
	</tbody>
</table>

field collection and laboratory routine identification of rhodiola crenulata

औषधीय सामग्रियों की पहचान दवा सुरक्षा का आधार और गारंटी है। अधिकांश वैज्ञानिक शोधकर्ता जड़ी-बूटियों की सरल, तेज, प्रभावी और सस्ती पहचान प्रक्रिया के पक्ष में हैं। Rhodiola crenulata एक पारंपरिक तिब्बती दवा है जो उच्च ऊंचाई पर उगाई जाती है, मुख्य रूप से तिब्बत, युन्नान और चीन के सिचुआन क्षेत्रों में वितरित की जाती है। Rhodiola crenulate कई bioactivity के पास, विरोधी भड़काऊ, विरोधी हाइपोक्सिया और एंटीऑक्सीडेंट गुण के रूप में, और विकास के लिए काफी क्षमता है. बढ़ती बाजार की मांग और संसाधन सामग्री में तेजी से कमी के साथ, Rhodiola crenulata के भ्रमित उत्पादों की एक बड़ी संख्या लोगों को परेशान किया गया है. इसलिए, यह प्रोटोकॉल नियमित प्रयोगशाला परीक्षण के साथ संयुक्त क्षेत्र में रोडियोला क्रेनुलाटा की पहचान के लिए एक मानक प्रक्रिया का परिचय देता है। निवास स्थान, सूक्ष्म विशेषताओं और पतली परत क्रोमैटोग्राफी का संयोजन निस्संदेह रोडियोला क्रेनुलता को जल्दी, कुशलतापूर्वक और आर्थिक रूप से पहचान लेगा, तिब्बती चिकित्सा के निरंतर विकास और औषधीय सामग्रियों के गुणवत्ता नियंत्रण में योगदान देगा।

Herbal medicine has a long history and rich application experience in China, and it was the first systematic recording in Shennong&#39;s herbal classic1. The discovery of artemisinin applied to malaria promoted the development of herbal medicine into a new stage1. The use of modern scientific technology to uncover the exact mechanism of herbal medicine increases the utilization rate and demand for herbal medicine, opening a new international market for it2,3,4. However, this has led to a series of negative effects. Non-professionals have a vague understanding of the characteristics of herbal medicine, which makes the use of herbal medicine face a huge safety risk5.
Rhodiola crenulata, one of the plants of the Rhodiola species, is mainly distributed in Tibet, northwest Yunnan, and western Sichuan of China (Figure 1)6,7. Rhodiola crenulata comprises salidroside, tyrosol, gallic acid, and other compounds for the treatment of hypoxic-related diseases through the function of &#34;invigorating qi and promoting blood circulation, clearing pulse and calming asthma&#34;8,9,10,11. Field investigation shows that Rhodiola crenulata can be found in the alpine talus zones, gully slopes, and rock crevices at an altitude of 4,000-5,600 m. Its growing environment is cold, full of sunshine and intense radiation, and it belongs to the alpine meadow ecosystem. Rhodiola crenulata can be distributed in lamellar and point-like populations according to the growth terrain, and gene flow can be carried out through cross-pollination.
The pollen abortion of the genus Rhodiola, illegality excavation, and degenerated ecological environment make Rhodiola crenulata an endangered species6,12. In view of the high medicinal value of Rhodiola crenulata, counterfeit products are expected to flow into the market. This article presents the habitat of Rhodiola crenulata and some convenient laboratory identification methods. Firstly, we observed the growth environment of Rhodiola crenulata and its medicinal properties. Secondly, the microstructure of medicinal powder was observed by microscope. The last step is the key point. The representative components of Rhodiola crenulata were separated and identified according to the different adsorption or dissolution properties of these components in a certain substance. DNA-based authentication or metabolomics analysis methods of medicinal plants are complicated and expensive13. These basic, convenient, and economical methods can quickly identify Rhodiola crenulata.

लेखक स्पॉटलाइट: Rhodiola crenulata के लिए पहचान के तरीके विकसित करना और इसके संसाधन वितरण और औषधीय प्रभावों की जांच करना

फील्ड संग्रह और प्रयोगशाला नियमित Rhodiola crenulata की पहचान

The scope of our research is centered on conducting a resource survey of ethnic medicine. Specifically, this study aims to develop a rapid and convenient methods for the identification of Rhodiola crenulata. The protocol we offer presents a cost-effective, straightforward, and rapid method for identifying various medicinal herbal materials.Our laboratory will concentrate its future research endeavors on investigation two primary areas, the distribution of research associated with Rhodiola crenulata and the pharmacological impacts of this species.

इस प्रयोगात्मक प्रोटोकॉल की पहचान और क्षेत्र में Rhodiola crenulate एकत्र करने का वर्णन करता है. Rhodiola crenulate अल्पाइन ताल क्षेत्रों में रहने के लिए जाता है, गली ढलानों, और उच्च ऊंचाई पर रॉक दरारें. निवास स्थान, पूर...

Watch this Scientific Journal Video about Field Collection and Laboratory Routine Identification of Rhodiola crenulata at JoVE.com

यहाँ, हम निवास स्थान से Rhodiola Crenulata की पहचान का वर्णन, संयंत्र आकृति विज्ञान, औषधीय गुणों, सूक्ष्म सुविधाओं, और पतली परत क्रोमैटोग्राफी .

The identification of medicinal materials is the premise and guarantee of drug safety. The majority of scientific researchers are bound to favor the ...

हमारे शोध का दायरा जातीय चिकित्सा के संसाधन सर्वेक्षण के संचालन पर केंद्रित है। विशेष रूप से, इस अध्ययन Rhodiola crenulata की पहचान के लिए एक तेजी से और सुविधाजनक तरीके विकसित करना है. प्रोटोकॉल हम प्रदान विभिन्न औषधीय हर्बल सामग्री की पहचान के लिए एक लागत प्रभावी, सीधा और तेजी से विधि प्रस्तुत करता है.हमारी प्रयोगशाला दो प्राथमिक क्षेत्रों की जांच पर अपने भविष्य के अनुसंधान प्रयासों पर ध्यान केंद्रित करेगी, Rhodiola crenulata और इस प्रजाति के औषधीय प्रभावों के साथ जुड़े अनुसंधान के वितरण.

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Research

JoVE Journal

Medicine

Field Collection and Laboratory Routine Identification of Rhodiola crenulata

The identification of medicinal materials is the premise and guarantee of drug safety. The majority of scientific researchers are bound to favor the simple, fast, effective, and inexpensive identification process of herbals. Rhodiola crenulata is a traditional Tibetan medicine grown at high altitudes, mainly distributed in Tibet, Yunnan, and Sichuan regions of China. Rhodiola crenulate possesses multiple bioactivities, such as anti-inflammatory, anti-hypoxia, and antioxidant properties, and has great potential for development. With the increasing market demand and a rapid decrease in resource content, a large number of confused products of Rhodiola crenulata have been troubling people. Therefore, this protocol introduces a standard process for the identification of Rhodiola crenulata in the field combined with routine laboratory testing. The combination of habitat, microscopic features, and thin-layer chromatography will undoubtedly identify Rhodiola crenulata quickly, efficiently, and economically, contributing to the continuous development of Tibetan medicine and the quality control of medicinal materials.

Here, we describe the identification of Rhodiola Crenulata from habitat, plant morphology, medicinal properties, microscopic features, and thin-layer chromatography.

Macroscopic and Microscopic Identification of Rhodiola crenulata Traits and Constituents

To begin, observe the appearance traits of Rhodiola Crenulata with the unaided eye and record the observations. Inhale near the plant to identify the fragrance smell. Next, place a small piece of the root in the mouth, then sip and chew for taste identification.Using a brush, remove the soil on the surface of the plant. Place the plant in an oven at 40 degrees Celsius and turn it every 24 hours. Then powder the dried medicinal materials using a powder machine and filter it through a medicated number three sieve.Spread the powder evenly on a clean slide using a dissecting needle covering 1/3 of the slide within a two millimeter area. Using a glass dropper, add a drop of deionized water to the powder and quickly place a cover glass using a tweezer. Next, place the slide onto the microscope platform.Adjust the light source and the coarse focus spiral to view the powder. Switch to a 40X objective lens and observe the starch granules. Again, spread the powder evenly on a slide using a dissecting needle, then add a drop of chloral hydrate to the powder.Hold the slide with a tweezer and heat on the alcohol lamp thrice for one second each. Add a drop of glycerin to the powder and quickly place a cover glass onto the drop. After adjusting the microscope settings as demonstrated previously, select a 40X objective lens to visualize the catheter, cork cells and fibers, along with the wood parenchyma cells and pigment block respectively.Microscopic examination of our Crenulata revealed key features with cork cells appearing brownish yellow, or colorless with polygonal shapes. Starch grains were observed as single or multiples with distinctive umbilical points and closely arranged spiral vessels. Additionally, xylem parenchyma cells were visualized as achromatic and oval, containing calcium oxalate sand crystals, and irregularly shaped brown red pigment blocks were identified.

Rhodiola crenulata लक्षण और घटकों की मैक्रोस्कोपिक और सूक्ष्म पहचान

Preparing and Analyzing Thin Layer Chromatography Samples of Rhodiola crenulata and Reference Compounds

To begin, weigh three grams of Rhodiola crenulata powder and transfer it into a 100-milliliter conical bottle. Using a large bowl pipette, add 25 milliliters of methanol into the bottle. Place the bottle in the ultrasonic instrument.Set the power to 250 watts, frequency to 40 kilohertz, time to 30 minutes, and start the ultrasonication. Next, rinse the outside of the bottle under running water until it reaches room temperature. Filter the solution through a 0.22-micron microporus membrane.Using a one-milliliter syringe, aspirate 800 microliters of liquid from the bottle and collect 400 microliters of the midstream solution into a chromatographic sample bottle. Measure and add one milligram of salidroside, 0.5 milligrams of tyrosol, and 0.5 milligrams of gallic acid into three separate one-milliliter volumetric flasks. Then, fill each flask to the scale marked with methanol.Sonicate the samples in the ultrasonic instrument using the conditions described previously. Following ultrasonication, aspirate 800 microliters of liquid from the flask. Filter the solution through a 0.22-micrometer microporous membrane and collect 400 microliters of filtrate into the bottle.Add trichloromethane, ethyl acetate, methanol, and formic acid on one side of the double tank chromatography cylinder. Shake the cylinder to mix the contents evenly and cover the upper cylinder head. Arrange the gallic acid, salidroside, tyrosol standard solutions, and our crenulata solution on the sample rack in positions A1 to A4.Place a 5x10-centimeter silicone sheet on the sampling table.Turn on the automatic sampling machine and open the air control valve. Press the Continue button for automatic sampling. After sampling, turn off the machine and air valve.Remove the silicone sheet from the machine. Put the silicone sheet into the other side of the double tank chromatography cylinder. Cover the upper cylinder head and pre-saturate for 20 minutes.Next, use a tweezer to hold the upper end of the thin layer plate and quickly put the silicone sheet into the development agent. Once the organic solvent evaporates, spray the silicone sheet with chromogenic solution. The thin layer chromatographic analysis showed that the R.crenulata sample appeared in spots of the same color, corresponding to the gallic acid, salidroside, and tyrosol standards.