Name: Autor destacado: Desarrollo de métodos de identificación para Rhodiola crenulata e investigación de su distribución de recursos y efectos farmacológicos
Uploaded: 2023-10-27T13:20:00
Description: Watch this Scientific Journal Video about Field Collection and Laboratory Routine Identification of Rhodiola crenulata at JoVE.com

Este trabajo contó con el apoyo de la Fundación Nacional de Ciencias Naturales de China (81973569, 82274207 y 82104533), el Proyecto de Promoción de la Investigación Xinglin Scholar de la Universidad de Chengdu de MTC (XKTD2022013) y el Programa Clave de Investigación y Desarrollo de Ningxia (2023BEG02012).

Rhodiola crenulata se recolecta en la montaña nevada de Zhuoda, condado de Ganzi, prefectura autónoma tibetana de Ganzi, provincia de Sichuan, China (N 31.44570°, E 99.96086°, 4892 m). Las plantas son autentificadas como genuinas por el profesor Yi Zhang de la Escuela de Medicina Étnica de la Universidad de Medicina Tradicional China de Chengdu.
1. Colección de Rhodiola crenulata
<ol><li>Fotografía el mapa del hábitat de Rhodiola crenulata....

Caracterización microscópica e identificación basada en TLC de crenulato de Rhodiola

<ol>
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K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Demystifying+traditional+herbal+medicine+with+modern+approach.">Demystifying traditional herbal medicine with modern approach.</a> Nature Plants. 3, 17109 (2017).</li><li>da Fonseca, L. R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Herbal+medicinal+products+from+Passiflora+for+anxiety:+An+unexploited+potential.">Herbal medicinal products from Passiflora for anxiety: An unexploited potential.</a> The Scientific World Journal. 2020, 6598434 (2020).</li><li>Aziato, L., Antwi, H. O. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Facilitators+and+barriers+of+herbal+medicine+use+in+Accra,+Ghana:+an+inductive+exploratory+study.">Facilitators and barriers of herbal medicine use in Accra, Ghana: an inductive exploratory study.</a> BMC Complementary and Alternative Medicine. 16, 142 (2016).</li><li>Tao, H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Rhodiola+species:+A+comprehensive+review+of+traditional+use,+phytochemistry,+pharmacology,+toxicity,+and+clinical+study.">Rhodiola species: A comprehensive review of traditional use, phytochemistry, pharmacology, toxicity, and clinical study.</a> Medicinal Research Reviews. 39 (5), 1779-1850 (2019).</li><li>Wang, Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+dietary+supplement+Rhodiola+crenulata+extract+alleviates+dextran+sulfate+sodium-induced+colitis+in+mice+through+anti-inflammation,+mediating+gut+barrier+integrity+and+reshaping+the+gut+microbiome.">The dietary supplement Rhodiola crenulata extract alleviates dextran sulfate sodium-induced colitis in mice through anti-inflammation, mediating gut barrier integrity and reshaping the gut microbiome.</a> Food &amp; Function. 12 (7), 3142-3158 (2021).</li><li>Xie, N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Rhodiola+crenulate+alleviates+hypobaric+hypoxia-induced+brain+injury+via+adjusting+NF-&#954;B/NLRP3-mediated+inflammation.">Rhodiola crenulate alleviates hypobaric hypoxia-induced brain injury via adjusting NF-&#954;B/NLRP3-mediated inflammation.</a> Phytomedicine. 103, 154240 (2022).</li><li>Wang, X., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Salidroside,+a+phenyl+ethanol+glycoside+from+Rhodiola+crenulata,+orchestrates+hypoxic+mitochondrial+dynamics+homeostasis+by+stimulating+Sirt1/p53/Drp1+signaling.">Salidroside, a phenyl ethanol glycoside from Rhodiola crenulata, orchestrates hypoxic mitochondrial dynamics homeostasis by stimulating Sirt1/p53/Drp1 signaling.</a> Journal of Ethnopharmacology. 293, 115278 (2022).</li><li>Ma, D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Chemical+characteristics+of+Rhodiola+Crenulata+and+its+mechanism+in+acute+mountain+sickness+using+UHPLC-Q-TOF-MS/MS+combined+with+network+pharmacology+analysis.">Chemical characteristics of Rhodiola Crenulata and its mechanism in acute mountain sickness using UHPLC-Q-TOF-MS/MS combined with network pharmacology analysis.</a> Journal of Ethnopharmacology. 294, 115345 (2022).</li><li>Wang, X., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Rhodiola+crenulata+attenuates+apoptosis+and+mitochondrial+energy+metabolism+disorder+in+rats+with+hypobaric+hypoxia-induced+brain+injury+by+regulating+the+HIF-1&#945;/microRNA+210/ISCU1/2(COX10)+signaling+pathway.">Rhodiola crenulata attenuates apoptosis and mitochondrial energy metabolism disorder in rats with hypobaric hypoxia-induced brain injury by regulating the HIF-1&#945;/microRNA 210/ISCU1/2(COX10) signaling pathway.</a> Journal of Ethnopharmacology. 241, 111801 (2019).</li><li>d'Avigdor, E., Wohlmuth, H., Asfaw, Z., Awas, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+current+status+of+knowledge+of+herbal+medicine+and+medicinal+plants+in+Fiche,+Ethiopia.">The current status of knowledge of herbal medicine and medicinal plants in Fiche, Ethiopia.</a> Journal of Ethnobiology and Ethnomedicine. 10, 38 (2014).</li><li>S&#225;nchez, M., Gonz&#225;lez-Burgos, E., Divakar, P. 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Chinese virtual herbarium Available from: <a target="_blank" href="https://www.cvh.ac.cn/">https://www.cvh.ac.cn/</a> (2023)</li><li>Cunningham, A. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=There+&amp;#34;ain't+no+mountain+high+enough&amp;#34;?:+The+drivers,+diversity+and+sustainability+of+China's+Rhodiola+trade.">There &amp;#34;ain't no mountain high enough&amp;#34;?: The drivers, diversity and sustainability of China's Rhodiola trade.</a> Journal of Ethnopharmacology. 252, 112379 (2020).</li><li>Wang, Q., Ruan, X., Jin, Z. H., Yan, Q. C., Tu, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Identification+of+Rhodiola+species+by+using+RP-HPLC.">Identification of Rhodiola species by using RP-HPLC.</a> Journal of Zhejiang University. Science. B. 6 (6), 477-482 (2005).</li><li>Dong, X., Guo, Y., Xiong, C., Sun, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Evaluation+of+two+major+Rhodiola+species+and+the+systemic+changing+characteristics+of+metabolites+of+Rhodiola+crenulata+in+different+altitudes+by+chemical+methods+combined+with+UPLC-QqQ-MS-based+metabolomics.">Evaluation of two major Rhodiola species and the systemic changing characteristics of metabolites of Rhodiola crenulata in different altitudes by chemical methods combined with UPLC-QqQ-MS-based metabolomics.</a> Molecules. 25 (18), 4062 (2020).</li><li>Zhao, W., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=HPLC+fingerprint+differentiation+between+natural+and+ex+situ+populations+of+Rhodiola+sachalinensis+from+Changbai+Mountain,+China.">HPLC fingerprint differentiation between natural and ex situ populations of Rhodiola sachalinensis from Changbai Mountain, China.</a> PloS One. 9 (11), 112869 (2014).</li><li>Yu, Y. Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Quality+evaluation+of+Tibetan+medicine+Rhodiolae+crenulatae+radix+et+rhizome+based+on+sensory+recognition+and+near+infrared+spectrum+analysis.">Quality evaluation of Tibetan medicine Rhodiolae crenulatae radix et rhizome based on sensory recognition and near infrared spectrum analysis.</a> Chengdu University of Traditional Chinese Medicine. , (2020).</li><li>Lv, X. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Simultaneous+determination+of+5+chemical+components+in+3+kinds+of+Rhodiola+rosea+by+HPLC.">Simultaneous determination of 5 chemical components in 3 kinds of Rhodiola rosea by HPLC.</a> China Pharmacy. 29 (18), 2515-2519 (2018).</li><li>Dong, T., Sha, Y., Liu, H., Sun, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Altitudinal+variation+of+metabolites,+mineral+elements+and+antioxidant+activities+of+Rhodiola+crenulata+(Hook.f.+&amp;+Thomson)+H.Ohba.">Altitudinal variation of metabolites, mineral elements and antioxidant activities of Rhodiola crenulata (Hook.f. &amp; Thomson) H.Ohba.</a> Molecules. 26 (23), 7383 (2021).</li><li>Ma, D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Application+of+UHPLC+fingerprints+combined+with+chemical+pattern+recognition+analysis+in+the+differentiation+of+six+Rhodiola+species.">Application of UHPLC fingerprints combined with chemical pattern recognition analysis in the differentiation of six Rhodiola species.</a> Molecules. 26 (22), 6855 (2021).</li><li>Li, X. H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Metabolic+discrimination+of+different+Rhodiola+species+using+1H-NMR+and+GEP+combinational+chemometrics.">Metabolic discrimination of different Rhodiola species using 1H-NMR and GEP combinational chemometrics.</a> Chemical &amp; Pharmaceutical Bulletin. 67 (2), 81-87 (2019).</li><li>Liu, Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Identification+of+Hippophae+species+(Shaji)+through+DNA+barcodes.">Identification of Hippophae species (Shaji) through DNA barcodes.</a> Chinese Medicine. 10, 28 (2015).</li></ol>

Hay más de 90 especies de plantas de Rhodiola en el mundo, y más del 60% de todas las especies se encuentran en China, las comunes incluyen Rhodiola crenulata, Rhodiola rosea, Rhodiolas achalinensis y Rhodiola algida17. Rhodiola crenulata, registrada en la primera parte de la Farmacopea China (2020), es una medicina tradicional tibetana cultivada a gran altitud. La demanda del mercado de Rhodiola crenulata aumenta cada año, por lo que garantizar el ...

La medicina herbal tiene una larga historia y una rica experiencia de aplicación en China, y fue el primer registro sistemático en el clásico herbal de Shennong1. El descubrimiento de la artemisinina aplicada a la malaria promovió el desarrollo de la medicina herbal en una nueva etapa1. El uso de la tecnología científica moderna para descubrir el mecanismo exacto de la medicina herbaria aumenta la tasa de utilización y la demanda de la medicina herbal, abriendo un nuevo mercado internacional para ella 2,3,4. Sin embargo, esto ha provocado una serie de efectos negativos. Los no profesionales tienen una comprensión vaga de las características de la fitoterapia, lo que hace que el uso de la fitoterapia se enfrente a un enorme riesgo de seguridad5.
Rhodiola crenulata, una de las plantas de la especie Rhodiola, se distribuye principalmente en el Tíbet, el noroeste de Yunnan y el oeste de Sichuan en China (Figura 1)6,7. La Rhodiola crenulata comprende salidrósido, tirosol, ácido gálico y otros compuestos para el tratamiento de enfermedades relacionadas con la hipóxica a través de la función de "vigorizar el qi y promover la circulación sanguínea, limpiar el pulso y calmar el asma"8,9,10,11. La investigación de campo muestra que Rhodiola crenulata se puede encontrar en las zonas de taludes alpinos, laderas de barrancos y grietas rocosas a una altitud de 4.000-5.600 m. Su entorno de cultivo es frío, lleno de sol y radiación intensa, y pertenece al ecosistema de la pradera alpina. Rhodiola crenulata puede distribuirse en poblaciones laminares y puntuales según el terreno de crecimiento, y el flujo génico puede llevarse a cabo a través de la polinización cruzada.
El aborto polínico del género Rhodiola, la ilegalidad de la excavación y el ambiente ecológico degenerado hacen de Rhodiola crenulata una especie en peligro de extinción 6,12. En vista del alto valor medicinal de la Rhodiola crenulata, se espera que los productos falsificados fluyan hacia el mercado. Este artículo presenta el hábitat de Rhodiola crenulata y algunos métodos convenientes de identificación de laboratorio. En primer lugar, observamos el entorno de crecimiento de Rhodiola crenulata y sus propiedades medicinales. En segundo lugar, se observó la microestructura del polvo medicinal al microscopio. El último paso es el punto clave. Los componentes representativos de Rhodiola crenulata se separaron e identificaron de acuerdo con las diferentes propiedades de adsorción o disolución de estos componentes en una determinada sustancia. Los métodos de autenticación basada en el ADN o análisis metabolómico de plantas medicinales son complicados y costosos13. Estos métodos básicos, convenientes y económicos pueden identificar rápidamente la Rhodiola crenulata.

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			<td>0.22 &#956;m millipore filter</td>
			<td>Millipore</td>
			<td>SLGP033RB</td>
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			<td>Automatic sampling machine</td>
			<td>CAMAG</td>
			<td>ATS 4</td>
			<td></td>
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			<td>Chloral hydrate</td>
			<td>Fuzhou Brunei Technology Co., Ltd</td>
			<td>ST1002</td>
			<td></td>
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			<td>Chromatographic&#160;sample&#160;bottles</td>
			<td>Zhejiang ALWSCI Technology Co., Ltd</td>
			<td>C0000008</td>
			<td></td>
		</tr>
		<tr>
			<td>Conical flask</td>
			<td>Sichuan Shubo Co., Ltd</td>
			<td>1121</td>
			<td></td>
		</tr>
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			<td>Cover glass</td>
			<td>Citotest Labware Manufacturing Co., Ltd</td>
			<td>10211818c</td>
			<td></td>
		</tr>
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			<td>Dissecting needle</td>
			<td>Shanghai Bingyu Fluid Technology Co., Ltd</td>
			<td>BY-5026</td>
			<td></td>
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		<tr>
			<td>Electronic balance</td>
			<td>SHIMADZU</td>
			<td>ATX124</td>
			<td></td>
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			<td>Ethyl acetate</td>
			<td>Chengdu Kelong Chemical Co., Ltd</td>
			<td>2022120901</td>
			<td></td>
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			<td>Formic acid</td>
			<td>Chengdu Kelong Chemical Co., Ltd</td>
			<td>2021110801</td>
			<td></td>
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			<td>Gallic acid</td>
			<td>Chengdu Herbpurify Co., Ltd</td>
			<td>M-017</td>
			<td></td>
		</tr>
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			<td>Glycerol</td>
			<td>Sinopharm Chemical Reagent Co., Ltd</td>
			<td>10010618</td>
			<td></td>
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			<td>High speed&#160; crusher</td>
			<td>Beijing Zhongxingweiye Instrument Co., Ltd</td>
			<td>FW-100</td>
			<td></td>
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			<td>Methanol</td>
			<td>Chengdu Kelong Chemical Co., Ltd</td>
			<td>20230108</td>
			<td></td>
		</tr>
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			<td>Microscope</td>
			<td>Chongqing Oprec Nistrument Co.,&#160; Ltd</td>
			<td>B203</td>
			<td></td>
		</tr>
		<tr>
			<td>Microscope slide</td>
			<td>Citotest Labware Manufacturing Co., Ltd</td>
			<td>7105P-G</td>
			<td></td>
		</tr>
		<tr>
			<td>Oven</td>
			<td>Shanghai Yuejin Medical Equipment Co., Ltd</td>
			<td>DHG-8145</td>
			<td></td>
		</tr>
		<tr>
			<td>Pharmacopoeia sieve</td>
			<td>Hangzhou Xingrun sieve factory</td>
			<td>572423281330</td>
			<td></td>
		</tr>
		<tr>
			<td>Pipette</td>
			<td>Changde BKMAM Biotechnology Co., Ltd</td>
			<td>120302008</td>
			<td></td>
		</tr>
		<tr>
			<td>Salidroside</td>
			<td>Chengdu Herbpurify Co., Ltd</td>
			<td>H-040</td>
			<td></td>
		</tr>
		<tr>
			<td>Saturate tank&#160;</td>
			<td>Yancheng Liegu Technology Co., Ltd</td>
			<td>10*20 P-1</td>
			<td></td>
		</tr>
		<tr>
			<td>Silica gel plate</td>
			<td>Yantai Jiangyou Silica Gel Development&#160; Co., Ltd</td>
			<td>HSG20211227</td>
			<td></td>
		</tr>
		<tr>
			<td>Trichloromethane</td>
			<td>Chengdu Kelong Chemical Co., Ltd</td>
			<td>20221013-1</td>
			<td></td>
		</tr>
		<tr>
			<td>Tweezer&#160;</td>
			<td>Changde BKMAM Biotechnology Co., Ltd</td>
			<td>130302027</td>
			<td></td>
		</tr>
		<tr>
			<td>Tyrosol</td>
			<td>Chengdu Herbpurify Co., Ltd</td>
			<td>L-042</td>
			<td></td>
		</tr>
		<tr>
			<td>Ultrasound equipment</td>
			<td>Ningbo Xinyi Ultrasonic Equipment Co., Ltd</td>
			<td>SB-8200DTS</td>
			<td></td>
		</tr>
		<tr>
			<td>Volumetric pipet</td>
			<td>Changde BKMAM Biotechnology Co., Ltd</td>
			<td>120301006</td>
			<td></td>
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field collection and laboratory routine identification of rhodiola crenulata

La identificación de los materiales medicinales es la premisa y garantía de la seguridad de los medicamentos. La mayoría de los investigadores científicos están obligados a favorecer el proceso de identificación simple, rápido, efectivo y económico de las hierbas. La Rhodiola crenulata es una medicina tradicional tibetana que se cultiva a gran altitud, distribuida principalmente en las regiones chinas de Tíbet, Yunnan y Sichuan. La crenulato de Rhodiola posee múltiples bioactividades, como propiedades antiinflamatorias, antihipoxia y antioxidantes, y tiene un gran potencial de desarrollo. Con el aumento de la demanda del mercado y una rápida disminución en el contenido de recursos, una gran cantidad de productos confusos de Rhodiola crenulata han estado preocupando a las personas. Por lo tanto, este protocolo introduce un proceso estándar para la identificación de Rhodiola crenulata en el campo combinado con pruebas de laboratorio de rutina. La combinación del hábitat, las características microscópicas y la cromatografía de capa delgada sin duda identificará a Rhodiola crenulata de manera rápida, eficiente y económica, contribuyendo al desarrollo continuo de la medicina tibetana y al control de calidad de los materiales medicinales.

Herbal medicine has a long history and rich application experience in China, and it was the first systematic recording in Shennong&#39;s herbal classic1. The discovery of artemisinin applied to malaria promoted the development of herbal medicine into a new stage1. The use of modern scientific technology to uncover the exact mechanism of herbal medicine increases the utilization rate and demand for herbal medicine, opening a new international market for it2,3,4. However, this has led to a series of negative effects. Non-professionals have a vague understanding of the characteristics of herbal medicine, which makes the use of herbal medicine face a huge safety risk5.
Rhodiola crenulata, one of the plants of the Rhodiola species, is mainly distributed in Tibet, northwest Yunnan, and western Sichuan of China (Figure 1)6,7. Rhodiola crenulata comprises salidroside, tyrosol, gallic acid, and other compounds for the treatment of hypoxic-related diseases through the function of &#34;invigorating qi and promoting blood circulation, clearing pulse and calming asthma&#34;8,9,10,11. Field investigation shows that Rhodiola crenulata can be found in the alpine talus zones, gully slopes, and rock crevices at an altitude of 4,000-5,600 m. Its growing environment is cold, full of sunshine and intense radiation, and it belongs to the alpine meadow ecosystem. Rhodiola crenulata can be distributed in lamellar and point-like populations according to the growth terrain, and gene flow can be carried out through cross-pollination.
The pollen abortion of the genus Rhodiola, illegality excavation, and degenerated ecological environment make Rhodiola crenulata an endangered species6,12. In view of the high medicinal value of Rhodiola crenulata, counterfeit products are expected to flow into the market. This article presents the habitat of Rhodiola crenulata and some convenient laboratory identification methods. Firstly, we observed the growth environment of Rhodiola crenulata and its medicinal properties. Secondly, the microstructure of medicinal powder was observed by microscope. The last step is the key point. The representative components of Rhodiola crenulata were separated and identified according to the different adsorption or dissolution properties of these components in a certain substance. DNA-based authentication or metabolomics analysis methods of medicinal plants are complicated and expensive13. These basic, convenient, and economical methods can quickly identify Rhodiola crenulata.

Autor destacado: Desarrollo de métodos de identificación para Rhodiola crenulata e investigación de su distribución de recursos y efectos farmacológicos

Recolección de campo e identificación rutinaria de laboratorio de Rhodiola crenulata

The scope of our research is centered on conducting a resource survey of ethnic medicine. Specifically, this study aims to develop a rapid and convenient methods for the identification of Rhodiola crenulata. The protocol we offer presents a cost-effective, straightforward, and rapid method for identifying various medicinal herbal materials.Our laboratory will concentrate its future research endeavors on investigation two primary areas, the distribution of research associated with Rhodiola crenulata and the pharmacological impacts of this species.

Este protocolo experimental describe la identificación y recolección de crenulato de Rhodiola en el campo. La crenula de Rhodiola tiende a vivir en las zonas de talud alpino, laderas de barrancos y grietas rocosas a grandes altitudes. El hábitat, la planta entera, la flor y las hojas de Rhodiola crenulate se pueden mostrar en la Figura 2. La crenula de Rhodiola tiene un rizoma de color marrón rojizo (Figura 3A). En la <str...

Watch this Scientific Journal Video about Field Collection and Laboratory Routine Identification of Rhodiola crenulata at JoVE.com

Aquí, describimos la identificación de Rhodiola Crenulata a partir del hábitat, la morfología de la planta, las propiedades medicinales, las características microscópicas y la cromatografía en capa fina.

The identification of medicinal materials is the premise and guarantee of drug safety. The majority of scientific researchers are bound to favor the ...

El alcance de nuestra investigación se centra en la realización de un estudio de recursos de medicina étnica. Específicamente, este estudio tiene como objetivo desarrollar métodos rápidos y convenientes para la identificación de Rhodiola crenulata. El protocolo que ofrecemos presenta un método rentable, sencillo y rápido para identificar diversos materiales herbales medicinales.Nuestro laboratorio concentrará sus futuros esfuerzos de investigación en dos áreas principales, la distribución de la investigación asociada a Rhodiola crenulata y los impactos farmacológicos de esta especie.

field-collection-laboratory-routine-identification-rhodiola

Research

JoVE Journal

Medicine

Field Collection and Laboratory Routine Identification of Rhodiola crenulata

The identification of medicinal materials is the premise and guarantee of drug safety. The majority of scientific researchers are bound to favor the simple, fast, effective, and inexpensive identification process of herbals. Rhodiola crenulata is a traditional Tibetan medicine grown at high altitudes, mainly distributed in Tibet, Yunnan, and Sichuan regions of China. Rhodiola crenulate possesses multiple bioactivities, such as anti-inflammatory, anti-hypoxia, and antioxidant properties, and has great potential for development. With the increasing market demand and a rapid decrease in resource content, a large number of confused products of Rhodiola crenulata have been troubling people. Therefore, this protocol introduces a standard process for the identification of Rhodiola crenulata in the field combined with routine laboratory testing. The combination of habitat, microscopic features, and thin-layer chromatography will undoubtedly identify Rhodiola crenulata quickly, efficiently, and economically, contributing to the continuous development of Tibetan medicine and the quality control of medicinal materials.

Here, we describe the identification of Rhodiola Crenulata from habitat, plant morphology, medicinal properties, microscopic features, and thin-layer chromatography.

Investigación

Medicina

Macroscopic and Microscopic Identification of Rhodiola crenulata Traits and Constituents

To begin, observe the appearance traits of Rhodiola Crenulata with the unaided eye and record the observations. Inhale near the plant to identify the fragrance smell. Next, place a small piece of the root in the mouth, then sip and chew for taste identification.Using a brush, remove the soil on the surface of the plant. Place the plant in an oven at 40 degrees Celsius and turn it every 24 hours. Then powder the dried medicinal materials using a powder machine and filter it through a medicated number three sieve.Spread the powder evenly on a clean slide using a dissecting needle covering 1/3 of the slide within a two millimeter area. Using a glass dropper, add a drop of deionized water to the powder and quickly place a cover glass using a tweezer. Next, place the slide onto the microscope platform.Adjust the light source and the coarse focus spiral to view the powder. Switch to a 40X objective lens and observe the starch granules. Again, spread the powder evenly on a slide using a dissecting needle, then add a drop of chloral hydrate to the powder.Hold the slide with a tweezer and heat on the alcohol lamp thrice for one second each. Add a drop of glycerin to the powder and quickly place a cover glass onto the drop. After adjusting the microscope settings as demonstrated previously, select a 40X objective lens to visualize the catheter, cork cells and fibers, along with the wood parenchyma cells and pigment block respectively.Microscopic examination of our Crenulata revealed key features with cork cells appearing brownish yellow, or colorless with polygonal shapes. Starch grains were observed as single or multiples with distinctive umbilical points and closely arranged spiral vessels. Additionally, xylem parenchyma cells were visualized as achromatic and oval, containing calcium oxalate sand crystals, and irregularly shaped brown red pigment blocks were identified.

Identificación macroscópica y microscópica de rasgos y constituyentes de Rhodiola crenulata

Preparing and Analyzing Thin Layer Chromatography Samples of Rhodiola crenulata and Reference Compounds

To begin, weigh three grams of Rhodiola crenulata powder and transfer it into a 100-milliliter conical bottle. Using a large bowl pipette, add 25 milliliters of methanol into the bottle. Place the bottle in the ultrasonic instrument.Set the power to 250 watts, frequency to 40 kilohertz, time to 30 minutes, and start the ultrasonication. Next, rinse the outside of the bottle under running water until it reaches room temperature. Filter the solution through a 0.22-micron microporus membrane.Using a one-milliliter syringe, aspirate 800 microliters of liquid from the bottle and collect 400 microliters of the midstream solution into a chromatographic sample bottle. Measure and add one milligram of salidroside, 0.5 milligrams of tyrosol, and 0.5 milligrams of gallic acid into three separate one-milliliter volumetric flasks. Then, fill each flask to the scale marked with methanol.Sonicate the samples in the ultrasonic instrument using the conditions described previously. Following ultrasonication, aspirate 800 microliters of liquid from the flask. Filter the solution through a 0.22-micrometer microporous membrane and collect 400 microliters of filtrate into the bottle.Add trichloromethane, ethyl acetate, methanol, and formic acid on one side of the double tank chromatography cylinder. Shake the cylinder to mix the contents evenly and cover the upper cylinder head. Arrange the gallic acid, salidroside, tyrosol standard solutions, and our crenulata solution on the sample rack in positions A1 to A4.Place a 5x10-centimeter silicone sheet on the sampling table.Turn on the automatic sampling machine and open the air control valve. Press the Continue button for automatic sampling. After sampling, turn off the machine and air valve.Remove the silicone sheet from the machine. Put the silicone sheet into the other side of the double tank chromatography cylinder. Cover the upper cylinder head and pre-saturate for 20 minutes.Next, use a tweezer to hold the upper end of the thin layer plate and quickly put the silicone sheet into the development agent. Once the organic solvent evaporates, spray the silicone sheet with chromogenic solution. The thin layer chromatographic analysis showed that the R.crenulata sample appeared in spots of the same color, corresponding to the gallic acid, salidroside, and tyrosol standards.