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Please note that all translations are automatically generated. Click here for the English version.
1.0K Views
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01:20 min
January 26th, 2024
DOI :
10.3791/65960-v
Transcript
人脑是一个复杂的器官,跨越不同的技能。要了解其功能,必须在整个大脑中构建详细的细胞传感器。我们的常规高通量方案允许以微米级分辨率分析人脑体积区域的 3D 细胞结构,从而实现其结构表征。
人脑大面积的体积重建提出了一些实验挑战,这些挑战与大脑样本的巨大尺寸、复杂的生物成分、可变的死后固定和储存条件以及来自脂褐素型色素的自发荧光信号有关。所有这些都会影响光学清除效率和展示质量。与其他清除技术相比,短组织转化方法与光片显微镜成像相结合,可用于快速同时处理多个人脑切片。
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