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Please note that all translations are automatically generated. Click here for the English version.
1.8K Views
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01:20 min
March 15th, 2024
DOI :
10.3791/65980-v
Transcript
铁载体与铁形成复合物,并在铁限制条件下将其转运到细胞中。铁载体是有效的毒力因子,有助于在宿主中建立严重感染,并且已知在调节铜绿假单胞菌的群体感应中发挥作用。我们的研究旨在定量估计假单胞菌中铁载体的产生。
铁载体是细胞外分子,因此使用游离上清液定量总铁载体以及吡喃蕊素和脓粒素。由于不能检测到小体积的脓蘑菇素,因此从大量无细胞上清液中提取。最近,LCMS和生物传感器已被开发用于定量化脓皮脂素。
LCMS对于小型实验室来说是一种昂贵的设置,而生物传感器只能定量化pyoch
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