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01:58 min
December 22nd, 2023
DOI :
10.3791/66193-v
Transcript
常驻血管壁CD34+干细胞因其在调节损伤后血管重塑中的关键作用而日益得到认可。建立一种稳定、高效的功能性CD34+细胞培养方法对于进一步研究不同生理和病理条件下的参与机制至关重要。该技术结合了磁珠筛选和流式细胞术,以纯化原代培养的常驻 CD34+ 干细胞。
然后,可以通过免疫荧光染色和钙成像对纯化的细胞进行功能鉴定。由于留留血管神经壁CD34+干细胞的表型性和功能异质性,因此有必要鉴定留留血管CD34+细胞的特定亚细胞类型。我们提出了一种用于血管壁驻留 CD34+ 干细胞的培养
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