Name: Forfatter Spotlight: Utvikling og karakterisering av miljøvennlige ligninbaserte mikropartikler for forbedret levering av bioflavonoider
Uploaded: 2024-03-01T14:05:00
Description: Watch this Scientific Journal Video about Author Spotlight: Development and Characterization of Eco-Friendly Lignin-Based Microparticles for Enhanced Delivery of Bioflavonoids at JoVE.com

Denne studien ble støttet av Bulgarian Scientific Fund under Contract No KΠ-06 H59/3 og av Scientific Project No. 07/2023 FVM, Trakia University.

1. Syntese av ligninmikropartikler
<ol><li>Tilbered en 50 mg / ml alkali lignin vandig oppløsning ved å oppløse 2,5 g alkalilignin i 50 ml ultrarent vann på en magnetomrører.</li>
	<li>Klargjør 1 % Tween 80-oppløsning ved å løse opp 1 ml Tween 80 i 100 ml ultrarent vann.</li>
	<li>Tilbered en 2 M oppløsning av HNO3 ved å fortynne 6,65 ml 67 % HNO3 (tetthet = 1,413 g/ml) med ultrarent vann til et endelig volum på 50 ml.</li>
	<li>Tilsett langsomt 15 ml av 1 % Tween 80 o...

Lignin mikro-/submikronpartikkelsyntese for frigjøring av bioflavonoider

<ol>
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A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Opportunities+and+challenges+for+lignin+valorization+in+food+packaging,+antimicrobial,+and+agricultural+applications.">Opportunities and challenges for lignin valorization in food packaging, antimicrobial, and agricultural applications.</a> Biomacromolecules. 24 (3), 1065-1077 (2023).</li><li>Aadil, K., Barapatre, A., Jha, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Synthesis+and+characterization+of+Acacia+lignin-gelatin+film+for+its+possible+application+in+food+packaging.">Synthesis and characterization of Acacia lignin-gelatin film for its possible application in food packaging.</a> Bioresour. 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K., Wyrobnik, T. A., Lam, A. T. -. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Emerging+trends+in+biodegradable+microcarriers+for+therapeutic+applications.">Emerging trends in biodegradable microcarriers for therapeutic applications.</a> Polymers. 15 (6), 1487 (2023).</li><li>Figueiredo, P., Lintinen, K., Hirvonen, J. T., Kostiainen, M. A., Santos, H. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Properties+and+chemical+modifications+of+lignin:+Towards+lignin-based+nanomaterials+for+biomedical+applications.">Properties and chemical modifications of lignin: Towards lignin-based nanomaterials for biomedical applications.</a> Prog. Mater. Sci. 93, 233-269 (2018).</li><li>Tang, Q., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Lignin-based+nanoparticles:+a+review+on+their+preparations+and+applications.">Lignin-based nanoparticles: a review on their preparations and applications.</a> Polymers. 12 (11), 2471 (2020).</li><li>Zhao, W., Simmons, B., Singh, S., Ragauskas, A., Cheng, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=From+lignin+association+to+nano-/micro-particle+preparation:+extracting+higher+value+of+lignin.">From lignin association to nano-/micro-particle preparation: extracting higher value of lignin.</a> Green Chemistry. 18 (21), 5693-5700 (2016).</li><li>Stewart, H., Golding, M., Matia-Merino, L., Archer, R., Davies, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Manufacture+of+lignin+microparticles+by+anti-solvent+precipitation:+Effect+of+preparation+temperature+and+presence+of+sodium+dodecyl+sulfate.">Manufacture of lignin microparticles by anti-solvent precipitation: Effect of preparation temperature and presence of sodium dodecyl sulfate.</a> Food Res Int. 66, 93-99 (2014).</li><li>Beisl, S., Friedl, A., Miltner, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Lignin+from+micro-+to+nanosize:+Applications.">Lignin from micro- to nanosize: Applications.</a> Int. J. Mol. Sci. 18, 2367 (2017).</li><li>Mishra, P. K., Ekielski, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+simple+method+to+synthesize+lignin+nanoparticles.">A simple method to synthesize lignin nanoparticles.</a> Colloids Interfaces. 3, 52 (2019).</li><li>Qian, Y., Deng, Y., Qiu, X., Li, H., Yang, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Formation+of+uniform+colloidal+spheres+from+lignin,+a+renewable+resource+recovered+from+pulping+spent+liquor.">Formation of uniform colloidal spheres from lignin, a renewable resource recovered from pulping spent liquor.</a> Green Chem. 16, 2156-2163 (2014).</li><li>Tardy, B. L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Lignin+nano-+and+microparticles+as+template+for+nanostructured+materials:+formation+of+hollow+metal-phenolic+capsules.">Lignin nano- and microparticles as template for nanostructured materials: formation of hollow metal-phenolic capsules.</a> Green Chem. 20, 1335-1344 (2018).</li><li>Silva, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Paraquat-loaded+alginate/chitosan+nanoparticles:+preparation,+characterization+and+soil+sorption+studies.">Paraquat-loaded alginate/chitosan nanoparticles: preparation, characterization and soil sorption studies.</a> J Haz Mat. 190 (1-3), 366-374 (2011).</li><li>Georgieva, N., Yaneva, Z. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Comparative+evaluation+of+natural+and+acid-modified+layered+mineral+materials+as+rimifon-carriers+using+UV/VIS,+FTIR,+and+equilibrium+sorption+study.">Comparative evaluation of natural and acid-modified layered mineral materials as rimifon-carriers using UV/VIS, FTIR, and equilibrium sorption study.</a> Cogent Chem. 1 (1), 1-16 (2015).</li><li>Zhang, P., Chen, D., Li, L., Sun, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Charge+reversal+nano-systems+for+tumor+therapy.">Charge reversal nano-systems for tumor therapy.</a> J Nanobiotechnol. 20, 31 (2022).</li><li>Yaneva, Z. L., Georgieva, N. V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Removal+of+diazo+dye+from+the+aqueous+phase+by+biosorption+onto+ball-milled+maize+cob+(BMMC)+biomass+of+Zea+mays.">Removal of diazo dye from the aqueous phase by biosorption onto ball-milled maize cob (BMMC) biomass of Zea mays.</a> Maced. J. Chem. Chem. Eng. 32 (1), 133-149 (2013).</li><li>Zatorska, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Drug-loading+capacity+of+polylactide-based+micro-+and+nanoparticles+-+Experimental+and+molecular+modeling+study.">Drug-loading capacity of polylactide-based micro- and nanoparticles - Experimental and molecular modeling study.</a> Int J Pharmaceutics. 591, 120031 (2020).</li><li>Yaneva, Z., Georgieva, N., Grumezescu, A. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Chapter+5+-+Physicochemical+and+morphological+characterization+of+pharmaceutical+nanocarriers+and+mathematical+modeling+of+drug+encapsulation/release+mass+transfer+processes.">Chapter 5 - Physicochemical and morphological characterization of pharmaceutical nanocarriers and mathematical modeling of drug encapsulation/release mass transfer processes.</a> Nanoscale Fabrication, Optimization, Scale-Up and Biological Aspects of Pharmaceutical Nanotechnology. , 173-218 (2018).</li><li>Yaneva, Z., Georgieva, N., Staleva, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Development+of+d,l-&#945;-tocopherol+acetate/zeolite+carrier+system:+equilibrium+study.">Development of d,l-&#945;-tocopherol acetate/zeolite carrier system: equilibrium study.</a> Monatshefte fur Chemie Chemical Monthly. 147 (7), 1167-1175 (2016).</li><li>Yaneva, Z., Georgieva, N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Study+on+the+physical+chemistry,+equilibrium,+and+kinetic+mechanism+of+Azure+A+biosorption+by+Zea+mays+biomass.">Study on the physical chemistry, equilibrium, and kinetic mechanism of Azure A biosorption by Zea mays biomass.</a> Journal of Dispersion Science and Technology. 35 (2), 193-204 (2014).</li></ol>

Blant de viktigste kritiske problemene med moderne syntesemetoder for utforming av legemiddelbærerformuleringer basert på biopolymerer, er anvendelsen av farlige organiske reagenser - flyktige og brannfarlige løsningsmidler, som tetrahydrofuran, aceton, metanol og til og med DMSO i høye konsentrasjoner - som begrenser deres anvendelighet i biomedisin, farmasøytisk industri og matteknologi på grunn av manifestasjonen av mulige toksiske effekter20, 21,22,23,...

I dag har tilbøyelighet til biopolymerer som cellulose, kitosan, kollagen, dextran, gelatin og lignin som forløpere for utforming av mikro- / submikronbærere med tilpassbar størrelse, fysisk-kjemiske egenskaper og biofunksjonaliteter økt i biomedisinsk, farmasøytisk og næringsmiddelteknologisk industri på grunn av deres anvendelighet innen vevsteknikk, 3D-bioprinting, in vitro sykdomsmodelleringsplattformer, emballasjeindustri, emulsjonsforberedelse og næringsmiddellevering blant annet 1,2,3.
Nye studier fremhever aspektene ved ligninbaserte hydrogeler samt mikro- og nanoformuleringer4 som fordelaktige kjøretøy som brukes til matemballasje5, energilagring6, kosmetikk7, termiske / lysstabilisatorer, forsterkede materialer og stoffbærermatriser8 for levering av hydrofobe molekyler, forbedring av UV-barrierer9, som forsterkende midler i nanokompositter, og som et alternativ til uorganiske nanopartikler på grunn av noen nylige sikkerhetsproblemer 10,11,12. Årsaken bak denne tendensen er biokompatibilitet, biologisk nedbrytbarhet og ikke-toksisitet av den naturlige heterobiopolymeren, samt dens påviste bioaktiviteter av lignin-antioksidantpotensial og radikal rensing, anti-proliferativ, og antimikrobiell aktivitet 13,14,15,16,17.
Vitenskapelig litteratur rapporterer ulike metoder for syntese (selvmontering, anti-løsningsmiddelutfelling, syreutfelling og løsningsmiddelskifting)18 og karakterisering av ligninbaserte mikro-/nanoskalerte formuleringer, inkludert anvendelse av dyre eller skadelige løsningsmidler som tetrahydrofuran (THF), dimetylsulfoksid (DMSO), N,N-dimetylformamid (DMF) og aceton, og kompliserte, indirekte og kjedelige prosesser som bruker mye utstyr og giftigestoffer12,19,20.
For å overvinne de sistnevnte ulempene, presenterer følgende protokoller nye metoder for syntese av ligninbaserte mikro-/submikronpartikler ved bruk av billige og grønne løsningsmidler; enkle, enkle, raske og følsomme prosesser som krever lite utstyr, giftfrie stoffer og enkle metoder for karakterisering og bestemmelse av innkapslingskapasitet mot dårlig vannløselige bioaktive forbindelser og in vitro-frigjøringspotensialet til ligninmatrisene. De presenterte laboratorieskala produksjonsmetodene er fordelaktige for fremstilling av funksjonelle ligninbærere med justerbare størrelser, høy innkapslingskapasitet og bærekraftig in vitro-frigjøringsadferd ved hjelp av enkle karakteriseringsprosedyrer og miljøvennlige kjemikalier som kan finne anvendelse på ulike områder av biomedisinsk vitenskap og matteknologi. To flavonoider ble påført som målmolekyler innkapslet i ligninpartiklene: morin i mikropartiklene og quercetin i submikronpartiklene. Forskjellen i strukturene til begge flavonoider er bare posisjonen til den andre -OH-gruppen i den B-aromatiske ringen: -OH-gruppen er på 2'-posisjonen i morin og på 3'-posisjonen i quercetin, og dermed er begge organiske forbindelser posisjonelle isomerer. Sistnevnte faktum forutsetter lignende oppførsel av begge bioaktive naturlige forbindelser i prosessene for innkapsling og / eller frigjøring.

<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>automatic-cell counter</td>
			<td>EVE, NanoEnTek</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Citric acid</td>
			<td>Sigma</td>
			<td>251275</td>
			<td>&#160;ACS reagent, &#8805;99.5%</td>
		</tr>
		<tr>
			<td>digital water bath</td>
			<td>Memmert</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Eppendorf tubes, 1.5-2 mL</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Ethanol</td>
			<td>Sigma</td>
			<td>34852-M</td>
			<td>absolute, suitable for HPLC, &#8805;99.8%</td>
		</tr>
		<tr>
			<td>Folin&#8211;Ciocalteu&#8217;s phenol reagent</td>
			<td>Sigma</td>
			<td>F9252</td>
			<td></td>
		</tr>
		<tr>
			<td>&#160;freeze dryer</td>
			<td>Biobase</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>gallic acid</td>
			<td>Sigma-</td>
			<td>BCBW7577</td>
			<td>monohydrate</td>
		</tr>
		<tr>
			<td>HCl</td>
			<td>Sigma</td>
			<td>258148</td>
			<td>ACS reagent, 37%</td>
		</tr>
		<tr>
			<td>HNO3</td>
			<td>Sigma</td>
			<td>438073</td>
			<td>&#160;ACS reagent, 70%</td>
		</tr>
		<tr>
			<td>lignin, alkali</td>
			<td>Sigma</td>
			<td>370959</td>
			<td></td>
		</tr>
		<tr>
			<td>morin</td>
			<td>Sigma</td>
			<td>PHL82601</td>
			<td></td>
		</tr>
		<tr>
			<td>NaCl</td>
			<td>Sigma</td>
			<td>S9888</td>
			<td>ACS reagent, &#8805;99.0%</td>
		</tr>
		<tr>
			<td>Na2CO3</td>
			<td>Sigma</td>
			<td>223530</td>
			<td>powder, &#8805;99.5%, ACS reagent</td>
		</tr>
		<tr>
			<td>NaOH</td>
			<td>Sigma</td>
			<td>655104</td>
			<td>reagent grade, 97%, powder</td>
		</tr>
		<tr>
			<td>orbital shaker</td>
			<td>IKA</td>
			<td>KS 130 basic</td>
			<td></td>
		</tr>
		<tr>
			<td>pH-meter</td>
			<td>Consort</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>phosphate-buffered saline (PBS)</td>
			<td>Sigma</td>
			<td>RNBH7571</td>
			<td></td>
		</tr>
		<tr>
			<td>Quercetin hydrate</td>
			<td>Sigma</td>
			<td>STBG3815V</td>
			<td></td>
		</tr>
		<tr>
			<td>statistical software for Excel</td>
			<td>Microsoft Corporation</td>
			<td></td>
			<td>XLSTAT&#160; Version 2022.4.5.</td>
		</tr>
		<tr>
			<td>Tween 80</td>
			<td>Sigma</td>
			<td>P8074</td>
			<td>BioXtra, viscous liquid</td>
		</tr>
		<tr>
			<td>ultracentrifuge</td>
			<td>Hermle</td>
			<td>Z 326 K</td>
			<td></td>
		</tr>
		<tr>
			<td>Ultrapure water system</td>
			<td>Adrona</td>
			<td>INTEGRITY+</td>
			<td></td>
		</tr>
		<tr>
			<td>ultrasound homogenizer</td>
			<td>Bandelin Sonopuls</td>
			<td>HD 2070</td>
			<td></td>
		</tr>
		<tr>
			<td>UV/Vis spectrophotometer</td>
			<td>Hach-Lange</td>
			<td>DR 5000</td>
			<td></td>
		</tr>
	</tbody>
</table>

green synthesis, characterization, encapsulation, and measurement of the release potential of novel alkali lignin micro-/submicron particles

Anvendeligheten av biopolymer mikro-/nanoteknologi i human, veterinærmedisin, farmasøytisk og matteknologi vokser raskt på grunn av det store potensialet for biopolymerbaserte partikler som effektive bæresystemer. Bruken av lignin som en basisk heteropolymerbiomatrise for utforming av innovative mikro-/submikronformuleringer gjør det mulig å oppnå økt biokompatibilitet og tilbyr ulike aktive funksjonelle grupper som gir muligheter for tilpasning av de fysisk-kjemiske egenskapene og bioaktivitetene til formuleringene for ulike anvendelser. Målet med denne studien var å utvikle en enkel og miljøvennlig metodikk for syntese av ligninpartikler med mikro- og submikronstørrelse; å evaluere deres fysisk-kjemiske, spektrale og strukturelle egenskaper; og å undersøke deres evne til innkapsling av biologisk aktive molekyler og potensial for in vitro frigjøring av bioflavonoider i simulerte gastrointestinale medier. De presenterte metodene bruker billige og grønne løsningsmidler; enkle, enkle, raske og følsomme prosesser som krever lite utstyr, giftfrie stoffer og enkle metoder for karakterisering av dem, bestemmelse av innkapslingskapasitet mot de dårlig vannløselige bioaktive forbindelsene morin og quercetin, og in vitro-frigjøringspotensialet til ligninmatrisene.

Nowadays inclination towards biopolymers such as cellulose, chitosan, collagen, dextran, gelatin, and lignin as precursors for the design of micro-/submicron carriers with customizable size, physicochemical properties, and biofunctionalities has increased in the biomedical, pharmaceutical, and food technology industries due to their applicability in tissue engineering, 3D bioprinting, in vitro disease modeling platforms, packaging industry, emulsion preparation, and nutrient delivery among others1,2,3.
Novel studies highlight the aspects of lignin-based hydrogels as well as micro- and nano- formulations4 as advantageous vehicles used for food packaging materials5, energy storage6, cosmetics7, thermal/light stabilizers, reinforced materials, and drug-carrier matrices8 for the delivery of hydrophobic molecules, improvement of UV barriers9, as reinforcing agents in nanocomposites, and as an alternative to inorganic nanoparticles due to some recent safety issues10,11,12. The reason behind this tendency is the biocompatibility, biodegradability, and non-toxicity of the natural hetero biopolymer, as well as its proven bioactivities of lignin-antioxidant potential and radical scavenging, anti-proliferative, and antimicrobial activities13,14,15,16,17.
Scientific literature reports various methods for synthesis (self-assembly, anti-solvent precipitation, acid precipitation, and solvent shifting)18 and characterization of lignin-based micro-/nano- scaled formulations, including the application of expensive or harmful solvents such as tetrahydrofuran (THF), dimethyl sulfoxide (DMSO), N,N-dimethylformamide (DMF), and acetone, and complicated, indirect, and tedious processes that use a lot of equipment and toxic substances12,19,20.
To overcome the latter disadvantages, the following protocols present novel methodologies for the synthesis of lignin-based micro-/submicron particles using cheap and green solvents; easy, straightforward, quick, and sensitive processes requiring little equipment, non-toxic substances, and simple methods for their characterization and the determination of encapsulation capacity towards poorly water-soluble bioactive compounds and in vitro release potential of the lignin matrices. The presented lab-scale production methods are advantageous for the manufacture of functional lignin carriers with tunable sizes, high encapsulation capacity, and sustainable in vitro release behavior utilizing simple characterization procedures and eco-friendly chemicals that can find application in various areas of biomedical sciences and food technology. Two flavonoids were applied as target molecules encapsulated into the lignin particles: morin-into the microparticles, and quercetin-into the submicron particles. The difference in the structures of both flavonoids Is only the position of the second -OH group in the B-aromatic ring: the -OH group is on the 2&#39; position in morin and on the 3&#39; position in quercetin, thus both organic compounds are positional isomers. The latter fact presumes similar behavior of both bioactive natural compounds in the processes of encapsulation and/or release.

Forfatter Spotlight: Utvikling og karakterisering av miljøvennlige ligninbaserte mikropartikler for forbedret levering av bioflavonoider

Grønn syntese, karakterisering, innkapsling og måling av frigjøringspotensialet til nye alkaliske lignin mikro-/submikronpartikler

The objective of this research was to create a straightforward and environmentally friendly process to produce small lignin particles. The study aimed to analyze their physical, chemical, and structural properties, as well as investigate their ability to encapsulate and release bioflavonoids in stimulated gastrointestinal environments. Our method synthesizes lignin based particles using cheap and green solvents.The process is easy, quick, and requires minimal equipment and non-toxic substances. It also has simple methods for characterization, encapsulation, capacity determination, and in-vitro release potential of poorly water soluble bioactive compounds. The innovative biopolymer microparticles are suitable for oral administration due to the lower risk of gastric irritation.The submicron particles, due to the small size and significant release potential, could be applied as injectable formulations.

En anti-løsningsmiddel utfellingsteknikk ble utført for å produsere alkali lignin mikro-/submikronpartikler. En vandig oppløsning av fortynnet uorganisk syre-salpetersyre / organisk syre-sitronsyre ble dispergert i en alkalilignin vandig løsning, beriket med et miljøvennlig overflateaktivt middel / etanol, noe som resulterte i gradvis utfelling av biopolymeroppløsningen, og etter sonikering ble det endelig produsert en suspensjon av kompakte mikro- / submikronpartikler (figur 1).
...

Watch this Scientific Journal Video about Author Spotlight: Development and Characterization of Eco-Friendly Lignin-Based Microparticles for Enhanced Delivery of Bioflavonoids at JoVE.com

Vi beskriver nye, enkle metoder for syntese og karakterisering av biokompatible lignin mikro- og submikronpartikler. Disse formuleringene gir en lettvint tilnærming for utnyttelse av heteropolymeren, samt et alternativ for rasjonell utforming av multifunksjonelle bærermatriser med potensiell anvendelighet i biomedisin, farmasøytisk teknologi og næringsmiddelindustrien.

The applicability of biopolymer micro-/nano- technology in human, veterinary medicine, pharmaceutical, and food technology is rapidly growing due to the ...

Målet med denne forskningen var å skape en enkel og miljøvennlig prosess for å produsere små ligninpartikler. Studien hadde som mål å analysere deres fysiske, kjemiske og strukturelle egenskaper, samt undersøke deres evne til å innkapsle og frigjøre bioflavonoider i stimulerte gastrointestinale miljøer. Vår metode syntetiserer ligninbaserte partikler ved hjelp av billige og grønne løsemidler.Prosessen er enkel, rask og krever minimalt med utstyr og giftfrie stoffer. Det har også enkle metoder for karakterisering, innkapsling, kapasitetsbestemmelse og in vitro-frigjøringspotensial for dårlig vannløselige bioaktive forbindelser. De innovative biopolymermikropartiklene er egnet for oral administrering på grunn av lavere risiko for gastrisk irritasjon.Submikronpartiklene, på grunn av den lille størrelsen og det betydelige frigjøringspotensialet, kan brukes som injiserbare formuleringer.

green-synthesis-characterization-encapsulation-measurement-release

Research

JoVE Journal

Chemistry

Green Synthesis, Characterization, Encapsulation, and Measurement of the Release Potential of Novel Alkali Lignin Micro-/Submicron Particles

The applicability of biopolymer micro-/nano- technology in human, veterinary medicine, pharmaceutical, and food technology is rapidly growing due to the great potential of biopolymer-based particles as effective carrier systems. The use of lignin as a basic heteropolymer biomatrix for the design of innovative micro-/submicron formulations allows the achievement of increased biocompatibility and offers various active functional groups presenting opportunities for customization of the physicochemical properties and bioactivities of the formulations for diverse applications. The aim of the present study was to develop a simple and ecofriendly methodology for the synthesis of lignin particles with micro- and submicron size; to evaluate their physicochemical, spectral, and structural characteristics; and to examine their capacity for encapsulation of biologically active molecules and potential for in vitro release of bioflavonoids in simulated gastrointestinal media. The presented methodologies apply cheap and green solvents; easy, straightforward, quick, and sensitive processes requiring little equipment, non-toxic substances, and simple methods for their characterization, the determination of encapsulation capacity towards the poorly water-soluble bioactive compounds morin and quercetin, and the in vitro release potential of the lignin matrices.

We describe novel, simple methodologies of synthesis and characterization of biocompatible lignin micro- and submicron particles. These formulations provide a facile approach for the utilization of the heteropolymer, as well as an alternative for the rational design of multifunctional carrier matrices with potential applicability in biomedicine, pharmaceutical technology, and the food industry.