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Please note that all translations are automatically generated. Click here for the English version.
137 Views
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01:00 min
May 17th, 2024
DOI :
10.3791/66320-v
Transcript
该方案演示了冗长而复杂的基因表达载体的构建。当 cDNA 单次 PCR 无法获得全长 DNA 片段,或体外多次插入同源重组无法获得全长基因表达载体时,该方法适用。目前,使用多插入片段连接克隆来获得全长 DNA 片段,以便克隆到合适的载体中。
该方法可实现靶基因的缺失或突变,并高效地将大量 DNA 片段连接到表达载体上。
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