Name: fabrication of the thermoplastic microfluidic channels
Uploaded: 2008-02-03T23:56:00
Description: Watch this Scientific Journal Video about Fabrication of the Thermoplastic Microfluidic Channels at JoVE.com

制造微流体通道在一个循环的聚烯烃（Zeonex ® 690R，吉恩化工有限公司，路易斯维尔，肯塔基州）。 Zeonex有136℃的玻璃化转变温度（Tg）和紫外线透明的，这是光在巨石形成多孔的用于化学至关重要。微结构微热压印一个尼科电铸主模，它具有负（反）微流体平台的功能。热压印完成如下： <ol><li>模具和聚合物衬底之间放置两个平行的金属压板。 </li><li>该压板加热压花温度（166℃）， ...

<ol>
	<li>Bhattacharyya, A., Klapperich, C. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mechanical+and+chemical+analysis+of+plasma+and+ultraviolet-ozone+surface+treatments+for+thermal+bonding+of+polymeric+microfluidic+devices.">Mechanical and chemical analysis of plasma and ultraviolet-ozone surface treatments for thermal bonding of polymeric microfluidic devices.</a> Lab Chip. 7, 876-882 (2007).</li></ol>

fabrication of the thermoplastic microfluidic channels

在我们的实验室，我们已经成功地分离出核酸，直接从人体的血液，尿液和大便使用聚合物/纳米粒子复合材料的微观溶解和固相萃取柱微升和submicroliter卷。回收的样本PCRable无需任何额外的清理，集中，小体积样品。在这里，我们将演示如何制造热塑性微流体芯片采用热压成型和热封。然后，我们将演示如何使用通过密封芯片执导的原位光接枝表面和聚合，形成的复合材料的固相柱。我们展示嫁接和碳纳米管/聚合物复合列细菌细胞裂解聚合。然后，我们显示芯片采用硅/聚合物复合材料柱从样品核酸的固相萃取的裂解过程。所附的协议包含如何使裂解和固相萃取柱的详细说明。

Watch this Scientific Journal Video about Fabrication of the Thermoplastic Microfluidic Channels at JoVE.com

Fabrication of the Thermoplastic Microfluidic Channels

在这里我们将演示如何制造热塑性微流体芯片采用热压成型和热封。然后，我们将演示如何使用通过密封芯片执导的原位光接枝表面和聚合，形成的复合材料的固相柱。

制备热塑性微流体通道

In our lab, we have successfully isolated nucleic acids directly from microliter and submicroliter volumes of human blood, urine and stool using ...

Research

JoVE Journal

Biology

Fabrication of a Microfluidic Device for the Compartmentalization of Neuron Soma and Axons

条块索玛的神经元和轴突的一个微流体设备制造

In this video, we demonstrate the technique of soft lithography with polydimethyl siloxane (PDMS) which we use to fabricate a microfluidic device for ...

科研

生物学

Non-plasma Bonding of PDMS for Inexpensive Fabrication of Microfluidic Devices

PDMS微流体装置的廉价制造的非等离子键合

In this video, we demonstrate how to use the neuron microfluidic device without plasma bonding.  In some cases it may be desirable to reversibly bond ...

Microfluidic Applications for Disposable Diagnostics

微流控一次性诊断的应用

In this interview, Dr. Klapperich discusses the fabrication of thermoplastic microfluidic devices and their application for development of new ...

Patch Clamp Recording of Ion Channels Expressed in  Xenopus Oocytes

补丁钳爪蟾卵母细胞中表达的离子通道

Since its development by Sakmann and Neher 1, 2, the patch clamp has become established as an extremely useful technique for electrophysiological ...

Fabrication of Amperometric Electrodes

安培电极的制备

Carbon fiber electrodes are crucial for the detection of catecholamine release from vesicles in single cells for amperometry measurements.  Here, we ...

Fabrication of Myogenic Engineered Tissue Constructs

制作生肌工程的组织结构

Despite the fact that electronic pacemakers are life-saving medical devices, their long-term performance in pediatric patients can be problematic owing to ...

The Microfluidic Probe: Operation and Use for Localized Surface Processing

微探针：操作和使用的局部表面处理

Microfluidic devices allow assays to be performed using minute amounts of sample and have recently been used to control the microenvironment of cells. ...

Mutagenesis and Functional Analysis of Ion Channels Heterologously Expressed in Mammalian Cells

异源表达在哺乳动物细胞离子通道的突变和功能分析

We will demonstrate how to study the functional effects of introducing a point mutation in an ion channel. We study G protein-gated inwardly rectifying ...

Patch Clamp and Perfusion Techniques for Studying Ion Channels Expressed in Xenopus oocytes

Patch Clamp and Perfusion Techniques for Studying Ion Channels Expressed in Xenopus oocytes

膜片钳和灌注技术研究离子通道的表达爪蟾卵母细胞

The protocol presented here is designed to study the activation of the large conductance, voltage- and Ca2+-activated K+ (BK) channels. The protocol may ...

A Fluorescent Screening Assay for Identifying Modulators of GIRK Channels

一个荧光识别GIRK通道调制器的筛选试验

G protein-gated inward rectifier K+ (GIRK) channels function as cellular mediators of a wide range of hormones and neurotransmitters and are expressed in ...