This study provides a rapid and robust protocol for the isolation of Campylobacter from food products. It overcomes several drawbacks of existing methods and offers advantages for the subsequent detection and characterization of isolate. Rinse aids in the field are often 10, 000 times larger than the samples used in food safety detection assays, such as PCR.
There's a need to reduce the error of sub-sampling in order to ensure the results are representative of the population of interest. This protocol has been used to isolate over 50 strains of Campylobacter, which have subsequently been utilized in genomic studies. Our data and analysis contributed to understanding the molecule mechanisms of Campylobacter host agents and horizontal gene transfer.
Such in size will facilitate the development of novel therapeutic strategies for managing Campylobacterosis. This protocol offers the opportunity to reduce sub sampling by a factor of 13 and also reduces the time required to isolate Campylobacter from raw meat by 24 hours or within an eight hour workday. Future studies can apply this methodology for the isolation of Campylobacter, not only from food products, but from environmental samples as well.
This can help eliminate false negative results and provide more accurate quantification of the number of contaminated samples.
