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Please note that all translations are automatically generated. Click here for the English version.
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02:29 min
February 2nd, 2024
DOI :
10.3791/66501-v
Transcript
DNA 双链断裂代表最危险的 DNA 损伤。作为回应,细胞采用两种主要的 DNA 双链断裂修复机制,即 NHEJ 和 HR。分别量化 NHEJ 和 HR 的效率对于探索与之相关的机制和因素至关重要。NHEJ 测定和 HR 测定是用于测量 NHEJ 和 HR 效率的既定方法。这些方法依赖于精心设计的质粒,这些质粒包含被破坏的绿色荧光蛋白基因,该基因具有用于诱导DSBs的核酸内切酶I-SceI的识别位点。
NHEJ 测定和 HR 测定可以使用染色体整合或染色体外方法进行。染色体整合
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