Research
Education
Sign In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
TR - Turkish
JA - Japanese
Please note that all translations are automatically generated. Click here for the English version.
215 Views
•
01:23 min
May 17th, 2024
DOI :
10.3791/66581-v
Transcript
功能基因组筛选为探测基因功能提供了一种强大的方法,并依赖于全基因组质粒文库的构建。传统的质粒文库构建方法既费时又费力。为了解决这个问题,我们开发了一种简单有效的方法,即基于CRISPR的模块化组装,或CRISPRmass。
通过CRISPR/Cas9切割cDNA或ORF质粒的共享载体序列,然后通过相邻组装插入UAS模块,通过CRISPRmass构建全基因组质粒库的过程被标准化为细菌转化前的大规模平行两步试管反应。CRISPRmass可以简单、快速、高效且经济高效地构建各种质粒文
Sign in or start your free trial to access this content
Explore More Videos
From the series
Privacy
Terms of Use
Policies
Contact Us
Recommend to library
JoVE NEWSLETTERS
JoVE Journal
Methods Collections
JoVE Encyclopedia of Experiments
Archive
JoVE Core
JoVE Business
JoVE Science Education
JoVE Lab Manual
Faculty Resource Center
Authors
Librarians
Access
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved