<meta charset="utf8"></head><body>İnsan SATMVEC'leri: İzolasyon ve Adiposit Çapraz Konuşma Çalışmaları

<ol>
	<li>Bierhansl, L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Central+role+of+metabolism+in+endothelial+cell+function+and+vascular+disease.">Central role of metabolism in endothelial cell function and vascular disease.</a> Physiology. 32 (2), 126-140 (2017).</li><li>Aird, W. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Endothelial+cell+heterogeneity.">Endothelial cell heterogeneity.</a> Cold Spring Harb Perspect Med. 2 (1), a006429 (2012).</li><li>Rajendran, P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+vascular+endothelium+and+human+diseases.">The vascular endothelium and human diseases.</a> Int J Biol Sci. 9 (10), 1057-1069 (2013).</li><li>Brown, O. I., Bridge, K., Kearney, M. T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Nicotinamide+adenine+dinucleotide+phosphate+oxidases+in+glucose+homeostasis+and+diabetes-related+endothelial+cell+dysfunction.">Nicotinamide adenine dinucleotide phosphate oxidases in glucose homeostasis and diabetes-related endothelial cell dysfunction.</a> Cells. 10 (9), 2315 (2021).</li><li>Luk, C., Haywood, N. J., Bridge, K. I., Kearney, M. T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Paracrine+role+of+the+endothelium+in+metabolic+homeostasis+in+health+and+nutrient+excess.">Paracrine role of the endothelium in metabolic homeostasis in health and nutrient excess.</a> Front Cardiovasc Med. 26 (9), 882923 (2022).</li><li>. Click-iT EdU Imaging Kits Available from: <a target="_blank" href="https://www.thermofisher.com/document-connect/document-connect.html?url=https://assets.thermofisher.com/TFS-Assets%2FLSG%2Fmanuals%2Fmp10338.pdf">https://www.thermofisher.com/document-connect/document-connect.html?url=https://assets.thermofisher.com/TFS-Assets%2FLSG%2Fmanuals%2Fmp10338.pdf</a> (2024)</li><li>Bou&#239;s, D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Endothelium+in+vitro:+A+review+of+human+vascular+endothelial+cell+lines+for+blood+vessel-related+research.">Endothelium in vitro: A review of human vascular endothelial cell lines for blood vessel-related research.</a> Angiogenesis. 4 (2), 91-102 (2001).</li><li>Ades, E. W., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=HMEC-1:+establishment+of+an+immortalized+human+microvascular+endothelial+cell+line.">HMEC-1: establishment of an immortalized human microvascular endothelial cell line.</a> J Invest Dermatol. 99 (6), 683-690 (1992).</li><li>Chi, J. T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Endothelial+cell+diversity+revealed+by+global+expression+profiling.">Endothelial cell diversity revealed by global expression profiling.</a> Proc Natl Acad Sci U S A. 100 (19), 10623-10628 (2003).</li><li>Brignole, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=213+ESC+Guidelines+on+cardiac+pacing+and+cardiac+resynchronization+therapy.">213 ESC Guidelines on cardiac pacing and cardiac resynchronization therapy.</a> Eur Heart J. 34 (29), 2281-2329 (2013).</li><li>Greenspon, A. J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Trends+in+permanent+pacemaker+implantation+in+the+United+States+from+1993+to+2009:+Increasing+complexity+of+patients+and+procedures.">Trends in permanent pacemaker implantation in the United States from 1993 to 2009: Increasing complexity of patients and procedures.</a> J Am Coll Cardiol. 60 (16), 1540-1545 (2012).</li><li>Mendis, S. A., Alwan, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Prioritized Research Agenda for Prevention and Control of Noncommunicable Diseases. , (2011).</li><li>Brown, O. I., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Relationship+among+diabetes,+obesity,+and+cardiovascular+disease+phenotypes:+A+UK+biobank+cohort+study.">Relationship among diabetes, obesity, and cardiovascular disease phenotypes: A UK biobank cohort study.</a> Diabetes Care. 46 (8), 1531-1540 (2023).</li></ol>

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studying adipose endothelial cell/adipocyte cross-talk in human subcutaneous adipose tissue

Endothelial cells (ECs) are squamous cells that line the inner surface of the blood vessel wall as a monolayer. They have many essential roles, including control of vascular tone, regulation of thrombosis, modulating the inflammatory response, and contributing to angiogenesis1. Given the importance of endothelial cells in cardiometabolic physiology, they are frequently used experimentally to further the understanding of pathophysiology and to examine new pharmacological treatments for cardiometabolic disease.
However, there is enormous heterogeneity in endothelial cell morphology, function, gene expression, and antigen composition depending on the origin of their vascular bed2. While endothelial cells from large arteries are best suited to atherosclerosis studies, endothelial cells from small vessels, known as microvascular endothelial cells (MVECs), are more suitable for angiogenesis studies2. Understanding the molecular basis for endothelial heterogeneity may provide valuable insights into vascular bed-specific therapies. Microvascular endothelial function also significantly differs in numerous diseases, including diabetes, cardiovascular disease, and systemic infection3,4. Therefore, the ability to isolate endothelial cells from defined patient groups allows direct comparison of their endothelial cell function and cellular cross-talk5. 
In this paper, we describe a novel method of isolating human MVECs from subcutaneous adipose tissue (hSATMVEC) taken at the time of cardiac implantable electronic device (CIED) insertion. hSATMVEC isolated following enzymatic digestion of subcutaneous adipose tissue (SAT) were grown and passaged on gelatin-coated plates. We then describe a range of phenotyping assays that have been successfully applied to hSATMVECs in order to validate their phenotype and demonstrate use in routine endothelial cell assays. Finally, we describe an application of hSATMVECs in an experimental model of hSATMVECs-adipocyte cross-talk.

<meta charset="utf8"></head><body>Yazar Spotlight: Deri Altı Yağ Dokusu Mikrovasküler Yüzey Çalışmaları ile Kardiyometabolik Hastalıklara Bakış

İnsan Subkutan Yağ Dokusunda Adipoz Endotel Hücresi/Adiposit Çapraz Konuşmasının İncelenmesi

We want to understand the role of the subcutaneous adipose tissue microvasculature in people with and without cardiometabolic disease. To do this, we've been isolating subcutaneous adipose tissue microvascular endothelial cells from patients undergoing cardiac implantable electronic devices. This is to study the endothelial cell phenotype and cell-cell crosstalk with adipocytes.Historically, we have used immortalized human cell lines or animal models to study cardiometabolic disease in vitro. However, these are not representative of diseases in human subjects. Our technique overcomes this by allowing us to understand the cellular basis of cardiometabolic disease using tissue from real-world patients.Our group is currently investigating the mechanism of dysregulated subcutaneous adipose tissue endothelial cell adipocyte crosstalk in people with diabetes, in particular, the impact of diabetic endothelium on adipocyte function and how this can be targeted to improve cardiometabolic health.

Read this Scientific Article Protocol about Studying Adipose Endothelial Cell/Adipocyte Cross-Talk in Human Subcutaneous Adipose Tissue at JoVE.com

Microvascular endothelial cells (MVECs) have many critical roles, including control of vascular tone, regulation of thrombosis, and angiogenesis. ...

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Studying Adipose Endothelial Cell/Adipocyte Cross-Talk in Human Subcutaneous Adipose Tissue

463 Views.  University of Leeds. We want to understand the role of the subcutaneous adipose tissue microvasculature in people with and without cardiometabolic disease. To do this, we've been isolating subcutaneous adipose tissue microvascular endothelial cells from patients undergoing cardiac implantable electronic devices. This is to study the endothelial cell phenotype and cell-cell crosstalk with adipocytes.Historically, we have used immortalized human cell lines or animal models to study cardiometabolic disease in...