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Isolation and Cryopreservation of Highly Viable Human Peripheral Blood Mononuclear Cells From Whole Blood: A Guide for Beginners

1.4K Views

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10:48 min

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October 25th, 2024

DOI :

10.3791/66794-v

October 25th, 2024

•

1,385 Views

Brian Dinh¹, Marten A. Hoeksema², Nathanael J. Spann³, Jacob Rendler¹, Isidoro Cobo⁴^,⁵, Christopher K. Glass³^,⁶, Calvin Yeang¹

¹Division of Cardiology, Department of Medicine, University of California San Diego, ²Department of Medical Biochemistry, Amsterdam Immunity and Infection: Inflammatory diseases; Amsterdam Cardiovascular Sciences: Atherosclerosis and Ischemic Syndrome, Amsterdam UMC location University of Amsterdam, ³Department of Cellular and Molecular Medicine, University of California San Diego, ⁴Division of Clinical Immunology & Rheumatology, Department of Medicine, Heersink School of Medicine, University of Alabama at Birmingham, ⁵Comprehensive Arthritis, Musculoskeletal, Bone and Autoimmunity Center, Heersink School of Medicine, University of Alabama at Birmingham, ⁶Department of Medicine, University of California San Diego

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Summary

Explore More Videos

Cryopreservation

Human Peripheral Blood Mononuclear Cells

Density Gradient Centrifugation

Dendritic Cells

Whole Blood Collection

Phlebotomy Technique

Centrifugation Protocol

Pellet Collection

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