Name: تسليط الضوء على المؤلف: استخدام نموذج بالون رأب الوريد في القوارض لمحاكاة التدخلات الجراحية للأوردة العميقة
Uploaded: 2024-05-24T14:30:00
Description: Balloon venoplasty is a commonly used clinical technique to treat deep vein stenosis and occlusion as a consequence of trauma, congenital anatomic ...

هذه الدراسة مدعومة من قبل منحة أبحاث العلوم الأساسية للمنتدى الوريدي الأمريكي (AVF) لعام 2023 و Surmodics، Inc.

يتبع البروتوكول التالي سياسات وإرشادات اللجنة المؤسسية لرعاية واستخدام (IACUC) بجامعة ميشيغان (UMICH) ، ومبادئ الحكومة الأمريكية لاستخدام ورعاية الفقارية المستخدمة في الاختبار والبحث والتدريب ، وإرشادات REACH 2.0. تتوافق اتفاقية ضمان رعاية UMICH مع OLAW و USDA وهي معتمدة بالكامل من قبل AAALAC International. تمت ا...

نموذج بالون رأب الوريد القوارض لدراسات علاج الأوردة العميقة

<ol>
	<li>Prandoni, P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+long-term+clinical+course+of+acute+deep+venous+thrombosis.">The long-term clinical course of acute deep venous thrombosis.</a> Ann Inter Med. 125 (1), 1-7 (1996).</li><li>Prandoni, P., Kahn, S. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Post-thrombotic+syndrome:+prevalence,+prognostication+and+need+for+progress.">Post-thrombotic syndrome: prevalence, prognostication and need for progress.</a> Br J Haematol. 145 (3), 286-295 (2009).</li><li>Gloviczki, P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+2023+Society+for+Vascular+Surgery,+American+Venous+Forum,+and+American+Vein+and+Lymphatic+Society+clinical+practice+guidelines+for+the+management+of+varicose+veins+of+the+lower+extremities.+Part+II.">The 2023 Society for Vascular Surgery, American Venous Forum, and American Vein and Lymphatic Society clinical practice guidelines for the management of varicose veins of the lower extremities. Part II.</a> J Vas Surg Venous Lymph Diso. 12 (1), 101670 (2024).</li><li>Henke, P., Sharma, S., Wakefield, T., Myers, D., Obi, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Insights+from+experimental+post-thrombotic+syndrome+and+potential+for+novel+therapies.">Insights from experimental post-thrombotic syndrome and potential for novel therapies.</a> Transl Res. 225, 95-104 (2020).</li><li>Diaz, J. A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Choosing+a+mouse+model+of+venous+thrombosis:+A+consensus+assessment+of+utility+and+application.">Choosing a mouse model of venous thrombosis: A consensus assessment of utility and application.</a> Arterioscl Thrombo Vasc Biol. 39 (3), 311-318 (2019).</li><li>Lee, Y. U., Lee, A. Y., Humphrey, J. D., Rausch, M. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Histological+and+biomechanical+changes+in+a+mouse+model+of+venous+thrombus+remodeling.">Histological and biomechanical changes in a mouse model of venous thrombus remodeling.</a> Biorheology. 52 (3), 235-245 (2015).</li><li>Xie, H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Correspondence+of+ultrasound+elasticity+imaging+to+direct+mechanical+measurement+in+aging+DVT+in+rats.">Correspondence of ultrasound elasticity imaging to direct mechanical measurement in aging DVT in rats.</a> Ultrasound Med Biol. 31 (10), 1351-1359 (2005).</li><li>Moini, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Venoplasty+and+stenting+in+post-thrombotic+syndrome+and+non-thrombotic+iliac+vein+lesion.">Venoplasty and stenting in post-thrombotic syndrome and non-thrombotic iliac vein lesion.</a> Minim Invasive Ther Allied Technol. 29 (1), 35-41 (2020).</li><li>Chaitidis, N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Management+of+post-thrombotic+syndrome:+A+comprehensive+review.">Management of post-thrombotic syndrome: A comprehensive review.</a> Curr Pharma Des. 28 (7), 550-559 (2022).</li><li>Dangas, G. D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=In-stent+restenosis+in+the+drug-eluting+stent+era.">In-stent restenosis in the drug-eluting stent era.</a> J Am Coll Cardiol. 56 (23), 1897-1907 (2010).</li><li>Negl&#233;n, P., Berry, M. A., Raju, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Endovascular+surgery+in+the+treatment+of+chronic+primary+and+post-thrombotic+iliac+vein+obstruction.">Endovascular surgery in the treatment of chronic primary and post-thrombotic iliac vein obstruction.</a> Euro J Vas Endovas Surg. 20 (6), 560-571 (2000).</li><li>Sharifi, M., Mehdipour, M., Bay, C., Smith, G., Sharifi, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Endovenous+therapy+for+deep+venous+thrombosis:+The+TORPEDO+trial.">Endovenous therapy for deep venous thrombosis: The TORPEDO trial.</a> Catheter Cardiovasc Interv. 76 (3), 316-325 (2010).</li><li>Nicklas, J. M., Gordon, A. E., Henke, P. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Resolution+of+deep+venous+thrombosis:+Proposed+immune+paradigms.">Resolution of deep venous thrombosis: Proposed immune paradigms.</a> Int J Mol Sci. 21 (6), 2080 (2020).</li><li>Li, J., Kim, S. G., Blenis, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Rapamycin:+One+drug,+many+effects.">Rapamycin: One drug, many effects.</a> Cell Metabol. 19 (3), 373-379 (2014).</li><li>Chen, X., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Rapamycin+regulates+Akt+and+ERK+phosphorylation+through+mTORC1+and+mTORC2+signaling+pathways.">Rapamycin regulates Akt and ERK phosphorylation through mTORC1 and mTORC2 signaling pathways.</a> Mol Carcinogen. 49 (6), 603-610 (2010).</li><li>Diaz, J. A., Farris, D. M., Wrobleski, S. K., Myers, D. D., Wakefield, T. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Inferior+vena+cava+branch+variations+in+C57BL/6+mice+have+an+impact+on+thrombus+size+in+an+IVC+ligation+(stasis)+model.">Inferior vena cava branch variations in C57BL/6 mice have an impact on thrombus size in an IVC ligation (stasis) model.</a> J Thromb Haemo. 13 (4), 660-664 (2015).</li><li>Sch&#246;nfelder, T., J&#228;ckel, S., Wenzel, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mouse+models+of+deep+vein+thrombosis.">Mouse models of deep vein thrombosis.</a> Gef&#228;sschirurgie. 22 (1), 28-33 (2017).</li><li>de Barros, R. S. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Morphometric+analysis+of+rat+femoral+vessels+under+a+video+magnification+system.">Morphometric analysis of rat femoral vessels under a video magnification system.</a> J Vasc Bras. 16 (1), 73-76 (2017).</li></ol>

يمكن إجراء VBM على الفئران ≥ 250 جم باستخدام بالونات في نطاق 2.5-3.5 ملم. بالنسبة للفئران التي تزيد عن 750 جراما ، فإن هذه التقنية محدودة بكمية أكبر من الأنسجة الدهنية في تجويف البطن وحجم العضو بدلا من التغيرات في قطر IVC. من تجربتنا ، وجدنا أن هناك حاجة إلى 10 أو أكثر لكل مجموعة للحصول على دلالة إحصائ...

انسداد الوريد العميق من الجلطات الدموية التي لم يتم حلها هي نتيجة شائعة لتجلط الأوردة العميقة (DVT) وهي أحد أسباب متلازمة ما بعد التخثر (PTS) ، والتي تكلف مليارات الدولارات في الرعاية الصحية وتؤثر بشكل كبير على نوعية الحياة1،2،3. PTS هو مرض التهابي فريد من نوعه مع تليف جدار الوريد4 الذي لم يتم التقاطه بالكامل في نماذج DVT الحيوانية الصغيرة5،6،7 ، والأهم من ذلك ، لا يمكن للنماذج السابقة تقييم الاستجابة للتدخلات العلاجية. غالبا ما يتم علاج العوائق الوريدية الخثارية عن طريق الدعامات ورأب الوريد بالبالون 8,9 ؛ يعوق هذه التدخلات ارتفاع معدل إعادة التدخل بسبب عودة التضيق والانسداد10،11،12.
على الرغم من توفر البالونات المغلفة بالأدوية لمعالجة تضيق / انسداد الشرايين ، لا يوجد أي منها للأوردة العميقة. الآلية الجزيئية وراء عودة التضيق غير مفهومة بشكل جيد في الأوردة وليس لها علاج مباشر4. لذلك ، هدفنا هو تقديم نموذج حيواني يمكنه اختبار العلاجات المقدمة محليا عبر بالونات رأب الوريد التي تحاكي انسداد الوريد العميق بعد الخثار. يعيد هذا النموذج إنتاج حالة ما بعد التخثر ويقيم كيفية تأثير الأنواع المختلفة من بالونات رأب الوريد على جدار الوريد في الجسم الحي.

<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>3-0 DemeCRYL</td>
			<td>DemeTech</td>
			<td>G183019F4P</td>
			<td>12 per box</td>
		</tr>
		<tr>
			<td>5-0 DemeCRYL</td>
			<td>DemeTech</td>
			<td>G285017B0P</td>
			<td>12 per box</td>
		</tr>
		<tr>
			<td>7-0 DemeLENE</td>
			<td>DemeTech</td>
			<td>PM197011F13M</td>
			<td>12 per box</td>
		</tr>
		<tr>
			<td>8-0 DemeLON</td>
			<td>DemeTech</td>
			<td>NL868007C7P</td>
			<td>12 per box</td>
		</tr>
		<tr>
			<td>Angled Clip Applier</td>
			<td>SCANLAN</td>
			<td>3795-01A</td>
			<td>7-1/4&#8243; (18.5 cm)</td>
		</tr>
		<tr>
			<td>Baxter 0.9% Sodium Chloride Irrigation Bottle</td>
			<td>Baxter</td>
			<td>SKU: 195-7124</td>
			<td>1000mL Bottle</td>
		</tr>
		<tr>
			<td>Bovie Cautery Low Temperature Fine Tip</td>
			<td>Symmetry Surgical Inc</td>
			<td>AA00</td>
			<td>10/bx</td>
		</tr>
		<tr>
			<td>Buprenorphine</td>
			<td>Fidelis Animal Health</td>
			<td>SKU- 099114</td>
			<td>1.3mg/mL, 3mL</td>
		</tr>
		<tr>
			<td>CD (Sprague Dawley) Rats</td>
			<td>Charles River</td>
			<td>Strain Code 001</td>
			<td>Male, 400 gm</td>
		</tr>
		<tr>
			<td>Clip-It Traceable Timer</td>
			<td>TRACEABLE PRODUCTS</td>
			<td>Cat. No. 5046</td>
			<td></td>
		</tr>
		<tr>
			<td>Cotton-Tip Applicator 3&#34;</td>
			<td>Dukal Corporation</td>
			<td>922-00037BX100</td>
			<td>1000/BOX</td>
		</tr>
		<tr>
			<td>Enzymatic Surgical Instrument Detergent and Presoak</td>
			<td>Medline</td>
			<td>MDS88000B9</td>
			<td>1 Gal</td>
		</tr>
		<tr>
			<td>Eye Lube</td>
			<td>Optixcare</td>
			<td>SKU-065441</td>
			<td>20gm&#160;</td>
		</tr>
		<tr>
			<td>Gaymar T/Pump Stryker Localized Warming/Cooling Therapy System</td>
			<td>Stryker</td>
			<td>TP700</td>
			<td></td>
		</tr>
		<tr>
			<td>Heifetz Microclips 12mmx2.25mm</td>
			<td>SCANLAN</td>
			<td>3795-20</td>
			<td>Straight</td>
		</tr>
		<tr>
			<td>Heifetz Microclips 12mmx2.25mm</td>
			<td>SCANLAN</td>
			<td>3795-28</td>
			<td>Curved</td>
		</tr>
		<tr>
			<td>Heifetz Microclips 8mmx1.75mm&#160;</td>
			<td>SCANLAN</td>
			<td>3795-14</td>
			<td>Straight</td>
		</tr>
		<tr>
			<td>Heifetz Microclips 8mmx1.75mm&#160;</td>
			<td>SCANLAN</td>
			<td>3795-18</td>
			<td>Curved</td>
		</tr>
		<tr>
			<td>Inflation device&#160;</td>
			<td>Merit Medical</td>
			<td>IN4130</td>
			<td>BasixCOMPAK with Analog Display</td>
		</tr>
		<tr>
			<td>Isoflurane</td>
			<td>MFR VETONE</td>
			<td>SKU-501017</td>
			<td>100mL, Liquid for Inhalation</td>
		</tr>
		<tr>
			<td>Microsurgery kit containing tweezers and forceps</td>
			<td>Customized</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Oxygen, E-tank</td>
			<td>Medline</td>
			<td>MDM1630</td>
			<td></td>
		</tr>
		<tr>
			<td>Sharpened Guidewire</td>
			<td>Customized</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Sterile Glad Press-n-Seal</td>
			<td>Glad</td>
			<td>SKU # PSS-140</td>
			<td>ETO Exposed</td>
		</tr>
		<tr>
			<td>Sterile Nonwoven Gauze Sponges 2s 4ply 4x4&#160;</td>
			<td>Medline</td>
			<td>SKU PRM21444H</td>
			<td>100Ct</td>
		</tr>
		<tr>
			<td>Surgical microscope&#160;</td>
			<td>Zeiss</td>
			<td>S100 / OPMI pico</td>
			<td></td>
		</tr>
		<tr>
			<td>Surgical Scrub &#38; Handwash</td>
			<td>Vetoquinol</td>
			<td>NDC: 17030-003-16</td>
			<td>16 oz</td>
		</tr>
		<tr>
			<td>Ultrasound Gel</td>
			<td>Medline</td>
			<td>CTR001305</td>
			<td>8.5 oz, 12/CS</td>
		</tr>
		<tr>
			<td>Ultrasound system</td>
			<td>Siemens</td>
			<td>ACUSON Antares</td>
			<td>3-D, Color, Continuous wave (CW), Pulsed wave (PW), Power CW/CW Spectral</td>
		</tr>
		<tr>
			<td>Venoplasty Balloon</td>
			<td>Customized</td>
			<td></td>
			<td></td>
		</tr>
	</tbody>
</table>

rodent inferior vena cava venoplasty balloon model

رأب الوريد بالبالون هو تقنية سريرية شائعة الاستخدام لعلاج تضيق الوريد العميق وانسداده نتيجة للصدمة أو التشوهات التشريحية الخلقية أو تجلط الأوردة العميقة الحاد (DVT) أو الدعامات. يتميز الانسداد الوريدي العميق المزمن من الناحية النسيجية بالجلطة أو التليف أو كليهما. حاليا ، لا يوجد علاج مباشر متاح لاستهداف هذه العمليات المرضية. لذلك ، من الضروري وجود نموذج حيواني موثوق به في الجسم الحي لاختبار التدخلات الجديدة. يسمح نموذج بالون رأب الوريد السفلي لبقاء القوارض (IVC) (VBM) بدراسة رأب الوريد بالبالون في الحالات غير الخثارية وما بعد الجلطة عبر نقاط زمنية متعددة. يمكن قياس التأثير الموضعي والنظامي لبالونات رأب الوريد المغلفة وغير المطلية عن طريق فحوصات الأنسجة والجلطة والدم مثل تفاعل البوليميراز المتسلسل في الوقت الفعلي (RT-PCR) ، واللطخة الغربية ، ومقايسة الممتز المناعي المرتبط بالإنزيم (ELISA) ، والتصوير الزيموي ، وجدار الوريد والتحليل الخلوي للخثرة ، وفحوصات الدم الكامل والبلازما ، والتحليل النسيجي. إن VBM قابل للتكرار ، ويكرر التدخلات البشرية الجراحية ، ويمكنه تحديد تغيرات بروتين الجلطات الدموية في جدار الوريد المحلي ، ويسمح بإجراء تحليلات متعددة من نفس العينة ، مما يقلل من عدد المطلوبة لكل مجموعة.

Deep vein obstructions from unresolved thrombi are a common consequence of deep vein thrombosis (DVT) and are one of the causes of post-thrombotic syndrome (PTS), costing billions of dollars in healthcare and significantly impacting the quality of life1,2,3. PTS is a unique inflammatory pathology with consequent vein wall fibrosis4 not fully captured in small animal DVT models5,6,7, and more importantly, previous models cannot evaluate the response to therapeutic interventions. Thrombotic venous obstructions are often treated by stenting and balloon venoplasty8,9; such interventions are hampered by a high reintervention rate due to restenosis and occlusion10,11,12.
Despite the availability of drug-coated balloons to address arterial-specific stenosis/occlusions, none exist for deep veins. The molecular mechanism behind restenosis is poorly understood in veins and has no direct therapy4. Therefore, our goal is to present an animal model that can test locally delivered therapeutics via venoplasty balloons simulating deep vein post-thrombotic obstructions. This model reproduces the post-thrombotic condition and evaluates how different types of venoplasty balloons affect the vein wall in vivo.

تسليط الضوء على المؤلف: استخدام نموذج بالون رأب الوريد في القوارض لمحاكاة التدخلات الجراحية للأوردة العميقة

القوارض الوريد السفلي الأجوف بالون الوريد نموذج

In our laboratory, led by Dr.Andrea Obi, we study a type of abnormal blood clotting, venous thrombosis. In fact, we're one of the very few laboratories in the world working in this promising discovery area. Our goal is to take our findings and translate them into new, safer, more effective approaches to treat venous thrombosis.Our work has led us to look more closely at the role of the immune system, including the presence of infection in thrombus formation, as well as the pathways leading to clot resolution, venous fibrosis, including vein wall scarring and post-thrombotic syndrome. No previous animal model has been able to replicate the complex immune interactions that occur in venous thromboembolism in humans. Additionally, no model has been able to characterize the response to endovascular treatments followed by ultrasound.We are making important contributions to understand how different types of immune cells interact with environment around a blood clot, and how those interactions can lead to changes in the expression of cell's genetics code, a process now as epigenetic regulation. We believe that Inferior Vena Cava Venoplasty Balloon Model will accelerate the development of novel balloon angioplasty prototypes for the treatment of venous thrombosis, and the possibility for prevention of post-thrombotic syndrome.

VBM هو نموذج يقيم تأثير بالونات رأب الوريد في السيطرة الكاملة على الإرقاء. أولا ، قمنا بتحديد قطر IVC (القسم الأوسط تحت الأرضي) ، كما هو موضح في الشكل 1 ، لتحديد حجم البالون الصحيح. ثانيا ، يتم توضيح تقنية الجراحة المجهرية المطورة خطوة بخطوة في الشكل 2 ، ونعرض أيض...

يقدم البروتوكول نموذجا للقوارض للبقاء على قيد الحياة لاختبار تدخلات بالون رأب الوريد (VB) في الأوردة العميقة غير الخثارية والخثارية وما بعد التخثر.

Balloon venoplasty is a commonly used clinical technique to treat deep vein stenosis and occlusion as a consequence of trauma, congenital anatomic ...

rodent-inferior-vena-cava-venoplasty-balloon-model

Research

JoVE Journal

Medicine

Rodent Inferior Vena Cava Venoplasty Balloon Model

Balloon venoplasty is a commonly used clinical technique to treat deep vein stenosis and occlusion as a consequence of trauma, congenital anatomic abnormalities, acute deep vein thrombosis (DVT), or stenting. Chronic deep venous obstruction is histopathologically characterized by thrombosis, fibrosis, or both. Currently, no direct treatment is available to target these pathological processes. Therefore, a reliable in vivo animal model to test novel interventions is necessary. The rodent survival inferior vena cava (IVC) venoplasty balloon model (VBM) allows the study of balloon venoplasty in non-thrombotic and post-thrombotic conditions across multiple time points. The local and systemic effect of coated and uncoated venoplasty balloons can be quantified via tissue, thrombus, and blood assays such as real-time polymerase chain reaction (RT-PCR), western blot, enzyme-linked immunosorbent assay (ELISA), zymography, vein wall and thrombus cellular analysis, whole blood and plasma assays, and histological analysis. The VBM is reproducible, replicates surgical human interventions, can identify local vein wall-thrombi protein changes, and allows multiple analyses from the same sample, decreasing the number of animals required per group.

The protocol presents a survival rodent model to test venoplasty balloon (VB) interventions in non-thrombotic, thrombotic, and post-thrombotic deep veins.

Developing a Rodent Venoplasty Balloon Model for Researching Deep Vein Stenosis Treatments

To begin, position the anesthetized rat for the ultrasound scanning of the inferior vena cava or IVC. Apply a conducting gel to the abdomen and orient the transducer in a transverse position. Using two-dimensional imaging mode or B mode, lower the 10 megahertz linear probe on the center of the abdomen.When the abdominal vessels come into view, differentiate the IVC from the abdominal aorta with compressibility and flow assessment. Once the IVC is located, identify the midsection in the middle third portion inferior to the renal veins and superior to the IVC bifurcation. At the midsection IVC, measure the wall to wall diameter in the transverse view using the cross-sectional B mode image, recording the widest diameter of the vessel.Store the data using compact disks as DICOM files. Clean the abdomen with gauze-soaked chlorhexidine scrub and chlorhexidine solution three times. Place the sterilized cling film as a surgical wrap to cover the rat thorax and abdominal area.Using iris scissors, make a three centimeter ventral midline incision approximately two centimeters below the xiphoid process through the skin and abdominal wall. With a sterile saline-soaked gauze, reflect the intestines to the animal's right side. Place a wire speculum in the incision to allow IVC visualization.Then using a sterile cotton tipped applicator, perform a blunt dissection. Employing a low temperature fine tip cautery, cauterize all IVC lumbar branches from the renal veins to the iliac bifurcation, and ligate side branches with 7-0 non-absorbable polypropylene sutures. Place a proximal curved vascular micro clip on the IVC, which is separated from the aorta and is inferior to the renal veins.Place a straight micro clip on the distal IVC superior to the iliac bifurcation. Place an 8-0 nylon U-stitch suture caudal to the left renal vein centered on the anterior surface of the IVC. Insert a 0.014 millimeter sharpened guide wire with the venoplasty balloon backloaded retrograde to blood flow into the infrarenal IVC caudal to the curved micro clip.Advance the balloon into the mid IVC using the Seldinger technique. Remove the sharpened guidewire and using a 20 milliliter inflation syringe, inflate the venoplasty balloon for three minutes with a 10 to 15%IVC overstretch. Flush sterile saline to all the systems before IVC cannulation to avoid air embolism.Tighten the U-stitch upon venoplasty balloon deflation and removal. Then remove the micro clips and close the laparotomy site in a two-layer fashion using a 3-0 polyglactin absorbable synthetic suture. Close both the abdominal wall and skin in a continuous pattern.Recover the rat in an individual cage and observe it for 30 minutes under a heating lamp placed 24 inches away from the cage before returning it to its original housing unit. Average pulsed wave doppler flow velocity in the inferior vena cava of the rats after clip placement of the vessel was significantly reduced.