Name: testing the physiological barriers to viral transmission in aphids using microinjection
Uploaded: 2008-05-14T16:16:00
Description: Watch this Scientific Journal Video about Testing the Physiological Barriers to Viral Transmission in Aphids Using Microinjection at JoVE.com

<ol><li>为了测试，如果是蚜虫能够到一个病毒载体，我们连喂2天受感染的组织上的蚜虫。 </li><li>然后，我们允许它在一个健康的植物饲料，5天。该厂然后熏蒸和3周后，我们开始寻找症状。 </li><li>当我们发现蚜虫不能载体的病毒，我们可以评估的2壁垒是阻止病毒使用显微注射过程。 </li><li>该病毒直接注射到hemocoel，从而绕过肠道传输障碍。 </li></ol>关于本议定书和昆虫取食植物免疫力的更多信息...

testing the physiological barriers to viral transmission in aphids using microinjection

马铃薯loafroll病毒（PLRV），从家庭Luteoviridae感染茄科植物。它是由蚜虫传播，主要是桃蚜。当未受感染的蚜虫在受感染的植物饲料，合同病毒通过植物的韧皮部。一旦食入，从昆虫肠道病毒必须通过血淋巴（昆虫血），然后必须通过唾液腺，为了传回一个新的工厂。蚜虫可采取不同的病毒，一种植物大嚼时，然而，只有一小部分将通过肠道和唾液腺，传输的两个主要障碍，感染更多的工厂。在实验室中，我们用酸浆植物研究PLRV传输。在这台主机，发育迟缓和interveinal萎黄（其余的绿色静脉血管之间的叶子发黄）的特点是症状。我们目前的视频演示不向量PLVR肠道是否是防止病毒传播的病毒和测试的昆虫蚜虫显微注射的方法。我们目前的视频演示不向量PLVR肠道或唾液腺是否是防止病毒传播的病毒和测试的昆虫蚜虫显微注射的方法。

Watch this Scientific Journal Video about Testing the Physiological Barriers to Viral Transmission in Aphids Using Microinjection at JoVE.com

Testing the Physiological Barriers to Viral Transmission in Aphids Using Microinjection

蚜虫是植物病毒的有效发射器。蚜虫显微注射的病毒程序，我们会告诉你今天，是一种技术，使研究人员能够直接注入到蚜虫hemocoel病毒，绕过肠道病毒传播的主要障碍，在circulative方式之一。同样的技术也可用于注入用于RNAi的dsRNA。

测试使用微量注射蚜虫病毒传播的生理障碍

Potato loafroll virus (PLRV), from the family Luteoviridae infects solanaceous plants. It is transmitted by aphids, primarily, the green peach aphid. When ...

Research

JoVE Journal

Biology

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测试的速度和运动方向的视觉灵敏度在蜥蜴

Testing visual sensitivity in any species provides basic information regarding behaviour, evolution, and ecology. However, testing specific features of ...

科研

生物学

Testing Nicotine Tolerance in Aphids Using an Artificial Diet Experiment

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产生稳定的转基因C.使用微量注射线虫

Transgenic Caenorhabditis elegans can be readily created via microinjection of a DNA plasmid solution into the gonad 1.  The plasmid DNA rearranges to ...

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一个磁性的系绳系统，以调查在果蝇的视觉和嗅觉介导的飞行控制

It has been clear for many years that insects use visual cues to stabilize their heading in a wind stream. Many animals track odors carried in the wind. ...

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High-throughput methods to examine protein localization or organelle morphology is an effective tool for studying protein interactions and can help ...

Activation of Apoptosis by Cytoplasmic Microinjection of Cytochrome c

Activation of Apoptosis by Cytoplasmic Microinjection of Cytochrome c

激活细胞凋亡的细胞色素的细胞质显微注射 C</em

Apoptosis, or programmed cell death, is a conserved and highly regulated pathway by which cells die1.  Apoptosis can be triggered when cells encounter a ...

Micro-Mechanical Characterization of Lung Tissue Using Atomic Force Microscopy

微型机械的肺组织利用原子力显微镜表征

Matrix stiffness strongly influences growth, differentiation and function of adherent cells1-3.  On the macro scale the stiffness of tissues and organs ...

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在早期鸡胚跟踪形态组织变形

Embryonic epithelia undergo complex deformations (e.g. bending, twisting, folding, and stretching) to form the primitive organs of the early embryo. ...

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The folding and assembly of proteins is essential for protein function, the long-term health of the cell, and longevity of the organism. Historically, the ...

Nephrotoxin Microinjection in Zebrafish to Model Acute Kidney Injury

肾毒素显微注射斑马鱼到模型急性肾损伤

The kidneys are susceptible to harm from exposure to chemicals they filter from the bloodstream. This can lead to organ injury associated with a rapid ...