Name: testing nicotine tolerance in aphids using an artificial diet experiment
Uploaded: 2008-05-14T16:17:00
Description: Watch this Scientific Journal Video about Testing Nicotine Tolerance in Aphids Using an Artificial Diet Experiment at JoVE.com

In this experiment, 4 adult aphids are enclosed in a small plastic cup. They feed from liquid diet pipetted between 2 layers of parafilm stretched over the top of the cup. After 3 days feeding, toxicity of diets containing different concentrations of nicotine is compared to control diets by counting adult and larval aphids in each cup.
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<li>Make solutions containing sucrose and 20 amino acids. Aphids can subsist on this diet, reproducing at normal levels, for at least 1 week. Please see attached file for the arti...

testing nicotine tolerance in aphids using an artificial diet experiment

Plants may upregulate the production of many different seconday metabolites in response to insect feeding. One of these metabolites, nicotine, is well know to have insecticidal properties. One response of tobacco plants to herbivory, or being gnawed upon by insects, is to increase the production of this neurotoxic alkaloid. Here, we will demonstrate how to set up an experiment to address this question of whether a tobacco-adapted strain of the green peach aphid, Myzus persicae, can tolerate higher levels of nicotine than the a strain of this insect that does not infest tobacco in the field.

Watch this Scientific Journal Video about Testing Nicotine Tolerance in Aphids Using an Artificial Diet Experiment at JoVE.com

Testing Nicotine Tolerance in Aphids Using an Artificial Diet Experiment

In this video and assay is demonstrated that tests the tolerance of nicotine to two types of aphids one that infests tobacco plants in the field and one that does not.

Plants may upregulate the production of many different seconday metabolites in response to insect feeding. One of these metabolites, nicotine, is well ...

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