Name: testing nicotine tolerance in aphids using an artificial diet experiment
Uploaded: 2008-05-14T16:17:00
Description: Watch this Scientific Journal Video about Testing Nicotine Tolerance in Aphids Using an Artificial Diet Experiment at JoVE.com

在这个实验中，4个成人蚜虫被封闭在一个小塑料杯。他们喂流质饮食，从2层的封口膜拉伸过的杯子的顶部之间吸管。经过3天的喂养，饮食含有不同浓度的尼古丁的毒性比较控制饮食计数在每杯成人和幼虫蚜虫。 <ol><li>设为含有蔗糖和20个氨基酸的解决方案。蚜虫，可以存在这样的饮食，在正常水平繁殖，至少1周。请参阅附加档案的人工饲料。 </li><li>分装成6种不同的Eppendorf管含有尼古丁1毫升...

testing nicotine tolerance in aphids using an artificial diet experiment

植物可能上调昆虫喂养了许多不同的seconday代谢产物的生产。这些代谢物，尼古丁，一个是知道有杀虫特性。烟草植物的一种反应，以草食动物，或咬昆虫后，增加生产这种神经毒性生物碱。在这里，我们将演示如何建立一个实验，以解决这个问题烟草适应株的桃蚜，桃蚜，是否能容忍更高水平的尼古丁比这种昆虫的应变不侵扰烟草该领域。

Watch this Scientific Journal Video about Testing Nicotine Tolerance in Aphids Using an Artificial Diet Experiment at JoVE.com

Testing Nicotine Tolerance in Aphids Using an Artificial Diet Experiment

在这个视频和检测表明，测试两种类型的蚜虫之一，在外地和一个不infests烟草的尼古丁耐受。

使用人工饲料实验测试蚜虫的尼古丁公差

Plants may upregulate the production of many different seconday metabolites in response to insect feeding. One of these metabolites, nicotine, is well ...

Research

JoVE Journal

Biology

Testing the Physiological Barriers to Viral Transmission in Aphids Using Microinjection

测试使用微量注射蚜虫病毒传播的生理障碍

Potato loafroll virus (PLRV), from the family Luteoviridae infects solanaceous plants. It is transmitted by aphids, primarily, the green peach aphid. When ...

科研

生物学

Morris Water Maze Experiment

Morris水迷宫实验

The Morris water maze is widely used to study spatial memory and learning. Animals are placed in a pool of water that is colored opaque with powdered ...

Bioenergetic Profile Experiment using C2C12 Myoblast Cells

使用C2C12成肌细胞的生物能简介实验

The ability to measure cellular metabolism and understand mitochondrial dysfunction, has enabled scientists worldwide to advance their research in ...

Isolation of Immune Cells from Primary Tumors

从原发肿瘤的免疫细胞的分离

Tumors create a unique immunosuppressive microenvironment (tumor microenvironment, TME) whereby leukocytes are recruited into the tumor by various ...

Using Microfluidic Devices to Measure Lifespan and Cellular Phenotypes in Single Budding Yeast Cells

使用微流体装置来衡量寿命和细胞表型单芽殖酵母细胞

Budding yeast Saccharomyces cerevisiae is an important model organism in aging research. Genetic studies have revealed many genes with conserved effects ...

Laboratory Protocol for Genetic Gut Content Analyses of Aquatic Macroinvertebrates Using Group-specific rDNA Primers

用基团特异 rDNA 引物对水生无脊椎动物进行遗传肠道含量分析的实验室规程

Analyzing food webs is essential for a better understanding of ecosystems. For example, food web interactions can undergo severe changes caused by the ...

Experimental Protocol for Using Drosophila As an Invertebrate Model System for Toxicity Testing in the Laboratory

Experimental Protocol for Using Drosophila As an Invertebrate Model System for Toxicity Testing in the Laboratory

利用果蝇作为无脊椎动物毒性试验模型系统的实验协议

Emergent properties and external factors (population-level and ecosystem-level interactions, in particular) play important roles in mediating ...

Optimized Scratch Assay for In Vitro Testing of Cell Migration with an Automated Optical Camera

Optimized Scratch Assay for In Vitro Testing of Cell Migration with an Automated Optical Camera

用自动光学相机进行细胞迁移的体外试验优化划痕试验

Cell migration is an important process that influences many aspects of health, such as wound healing and cancer, and it is, therefore, crucial for ...

A Blood-Free Diet to Rear Anopheline Mosquitoes

后尾性异草蚊子的无血饮食

Malaria research requires large-scale breeding and production conditions for mosquitoes (Anopheles spp.) in captivity. The sustainable and reliable ...

Investigating Zinc Transport Mechanism in Mammalian Cells with FluoZin-3 Assay

Characterizing Mammalian Zinc Transporters Using an In Vitro Zinc Transport Assay

Exploring Cellular Zinc Regulation Through ZnT1 Functionality (Video) | JoVE

使用 体外 锌转运测定表征哺乳动物锌转运蛋白

Transition metals such as Zn2+ ions must be tightly regulated due to their cellular toxicity. Previously, the activity of Zn2+ transporters was measured ...