Name: principles of site-specific recombinase (ssr) technology
Uploaded: 2008-05-29T12:11:00
Description: Watch this Scientific Journal Video about Principles of Site-Specific Recombinase SSR Technology at JoVE.com

principles of site-specific recombinase (ssr) technology

网站具体的重组（SSR）技术，使操纵基因的结构，探索基因功能和分子生物学已成为不可或缺的工具。网站，具体的重组酶的蛋白质结合，以不同的目标DNA序列。在1980年代在噬菌体的Cre / lox系统首次描述。 Cre重组是我型拓扑异构酶催化两个loxP位（X - OVER的P1位点）的网站站点特定的DNA重组。的Cre / lox系统不需要任何辅助因子。 LoxP位序列中包含不同的结合位点的Cre重组酶，环绕一个方向性的核心序列重组和重排的发生。当细胞中含有loxP位的网站，并表达Cre重组，重组事件发生。双链DNA Cre重组两个loxP位网站被切断，重新排列，并结扎（“剪刀和胶水”）。产品的重组事件的非对称序列的相对方向取决于。 SSR技术是经常使用的一种工具，以探讨基因的功能。这里感兴趣的基因表达Cre目标网站loxP位（“floxed”）两侧。动物，然后越过一个组织特异性启动子的控制下表达Cre重组酶的动物。组织中的表达Cre重组目标序列结合，并切除floxed基因。控制基因的缺失使基因在特定的组织和功能在不同的时间点的调查。采用SSR技术---有条件的突变的基因功能分析 - 已超过传统的敲奏通常是致命的基因缺失的显着优势。

Watch this Scientific Journal Video about Principles of Site-Specific Recombinase SSR Technology at JoVE.com

Principles of Site-Specific Recombinase SSR Technology

特定网站的重组（SSR）技术的Cre / lox系统的问世使在分子生物学的许多进步，并已证明本身作为一个有价值的工具，用于评估转基因动物的基因功能。这次采访，讨论了环化重组（CRE）以及如何使用这种酶，导致有条件的突变，已超过传统的敲了战略的显着优势的发展机制网站的特定重组。

特定地点的重组酶（SSR）技术的原则

Site-specific recombinase (SSR) technology allows the manipulation of gene structure to explore gene function and has become an integral tool of molecular ...

Research

JoVE Journal

Biology

Principles of Site-Specific Recombinase (SSR) Technology

Molecular Evolution of the Tre Recombinase

TRE重组酶的分子进化

Here we report the generation of Tre recombinase through directed, molecular evolution. Tre recombinase recognizes a pre-defined target sequence within ...

科研

生物学

Interview: HIV-1 Proviral DNA Excision Using an Evolved Recombinase

专访：HIV - 1前病毒DNA切除使用一个演进的重组酶

HIV-1 integrates into the host chromosome of infected cells and persists as a provirus flanked by long terminal repeats. Current treatment strategies ...

Closed System Cell Culture Protocol Using HYPERStack Vessels with Gas Permeable Material Technology

封闭的系统使用透氧材料科技HYPERStack船只的细胞培养协议

Large volume adherent cell culture is currently standardized on stacked plate cell growth products when microcarrier beads are not an optimal choice.   ...

Principles of Rodent Surgery for the New Surgeon

鼠类外科的原则，为新的外科医生

For both scientific and animal welfare reasons, training in basic surgical concepts and techniques should be undertaken before ever seeking to perform ...

Discovering Protein Interactions and Characterizing Protein Function Using HaloTag Technology

发现蛋白质相互作用和检定蛋白质功能使用的HaloTag技术

Research in proteomics has exploded in recent years with advances in mass spectrometry capabilities that have led to the characterization of numerous ...

Reduced-gravity Environment Hardware Demonstrations of a Prototype Miniaturized Flow Cytometer and Companion Microfluidic Mixing Technology

样机小型化流式细胞仪及配套的混合微流控技术降低重力环境硬件演示

Until recently, astronaut blood samples were collected in-flight, transported to earth on the Space Shuttle, and analyzed in terrestrial laboratories. If ...

Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control

一个定量聚合酶重组酶扩增法的发展与内部阳性对照

It was recently demonstrated that recombinase polymerase amplification (RPA), an isothermal amplification platform for pathogen detection, may be used to ...

Inducing a Site Specific Replication Blockage in E. coli Using a Fluorescent Repressor Operator System

Inducing a Site Specific Replication Blockage in E. coli Using a Fluorescent Repressor Operator System

诱导位点特异性复制遇阻<i&gt;电子。大肠杆菌</i&gt;用荧光阻遏操作员系统

Obstacles present on DNA, including tightly-bound proteins and various lesions, can severely inhibit the progression of the cell&#39;s replication ...

Quantification of Site-specific Protein Lysine Acetylation and Succinylation Stoichiometry Using Data-independent Acquisition Mass Spectrometry

用数据无关采集质谱法定量测定点特异蛋白赖氨酸乙酰化和琥珀酰化化学计量学

Post-translational modification (PTM) of protein lysine residues by N&#400;-acylation induces structural changes that can dynamically regulate protein ...

Parallel High Throughput Single Molecule Kinetic Assay for Site-Specific DNA Cleavage

平行高通量单分子动力学测定，用于现场特异性DNA裂解

Site-specific DNA cleavage (SSDC) is a key step in many cellular processes, and it is crucial to gene editing. This work describes a kinetic assay capable ...