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DOI :
10.3791/759-v
•
16:01 min
June 20th, 2008
Chapters
0:00
Title/ Prologue
0:56
Introduction
1:22
Separating Protein Samples by Gel Electrophoresis
2:06
Assembling the Immunoblot Sandwich
5:43
Transferring Proteins from Gel to Membrane
7:52
Immunoprobing Membrane with Directly Conjugated Secondary Antibody
10:45
Immunoprobing with Avidin-Biotin Coupling to Secondary Antibody
14:26
Visualization of Immunoblot with Chromogenic Substrates
15:32
Conclusion
免疫印迹法(Western印迹)是一个快速,灵敏的蛋白质,利用固有的特异性抗原抗体识别检测和表征的检测。这个视频提供蛋白质分离协议,印迹到膜蛋白,immunoprobing,和可视化,使用显色或化学发光基板。
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