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DOI :
10.3791/759-v
•
16:01 min
June 20th, 2008
Chapters
0:00
Title/ Prologue
0:56
Introduction
1:22
Separating Protein Samples by Gel Electrophoresis
2:06
Assembling the Immunoblot Sandwich
5:43
Transferring Proteins from Gel to Membrane
7:52
Immunoprobing Membrane with Directly Conjugated Secondary Antibody
10:45
Immunoprobing with Avidin-Biotin Coupling to Secondary Antibody
14:26
Visualization of Immunoblot with Chromogenic Substrates
15:32
Conclusion
(ウェスタンブロット)免疫ブロッティングは、抗原抗体認識に内在する特異性を悪用することによって機能するタンパク質の検出と特性評価のための迅速かつ高感度アッセイである。このビデオでは、タンパク質分離のためのプロトコール、メンブレン上にブロッティングタンパク質、immunoprobing、および発色または化学発光基質を用いて可視化を提供します。
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