Name: phase contrast and differential interference contrast (dic) microscopy
Uploaded: 2008-08-06T17:41:00
Duration: 6 min 49 s
Description: Watch this Scientific Journal Video about Phase Contrast and Differential Interference Contrast DIC Microscopy at JoVE.com

几个视频剪辑显示相衬，DIC的显微镜，是从另一个视频文章，题为<a href="details.stp?ID=197">“共生</a>层。朱庇特殷勤地承认这方面的贡献利德贝特实验室。

这个实验方法的完整的文本协议<a href="http://www.mrw.interscience.wiley.com/emrw/9780471143031/cp/cpcb/article/cb0401/current/abstract ">是可在细胞生物学中</a>的电流协议。

The authors have nothing to disclose.

phase contrast and differential interference contrast (dic) microscopy

相衬显微镜通常是用于生产透明，非光吸收，生物标本的对比。该技术被发现泽尼克，于1942年，谁获得诺贝尔和平奖为他的成就。 DIC显微镜，在20世纪60年代末引入，已在生物医学研究中流行，因为它突出标本结构细节的边缘，提供高分辨率光学厚标本的部分，包括组织细胞，蛋，和胚胎，并且不会受到从典型的阶段晕相衬图像。该协议凸显了这些显微技术的原则和实际应用。

Watch this Scientific Journal Video about Phase Contrast and Differential Interference Contrast DIC Microscopy at JoVE.com

Phase Contrast and Differential Interference Contrast DIC Microscopy

该协议凸显的相位和微分干涉对比的原则和实际应用（DIC）显微镜

相衬，微分干涉对比（DIC）显微镜

Phase-contrast microscopy is often used to produce contrast for transparent, non light-absorbing, biological specimens. The technique was discovered by ...

Research

JoVE Journal

Biology

Phase Contrast and Differential Interference Contrast (DIC) Microscopy

Labeling hESCs and hMSCs with Iron Oxide Nanoparticles for Non-Invasive in vivo Tracking with MR Imaging

氧化铁标记的胚胎干细胞和的hMSCs纳米粒子在体内的跟踪与非侵入性磁共振成像

In recent years, stem cell research has led to a better understanding of developmental biology, various diseases and its potential impact on regenerative ...

科研

生物学

Labeling Stem Cells with Fluorescent Dyes for non-invasive Detection with Optical Imaging

与非侵入性检测荧光染料标记的干细胞与光学成像

Optical imaging (OI) is an easy, fast and inexpensive tool for in vivo monitoring of new stem cell based therapies. The technique is based on ex vivo ...

RNAi Interference by dsRNA Injection into Drosophila Embryos

RNAi Interference by dsRNA Injection into Drosophila Embryos

RNAi的干扰双链RNA注射到果蝇胚胎

Genetic screening is one of the most powerful methods available for gaining insights into complex biological process 1. Over the years many improvements ...

Preparation, Purification, and Characterization of Lanthanide Complexes for Use as Contrast Agents for Magnetic Resonance Imaging

制备，纯化和磁共振成像造影剂的使用稀土配合物的表征

Polyaminopolycarboxylate-based ligands are commonly used to chelate lanthanide ions, and the resulting complexes are 
useful as contrast agents for ...

In vivo Clonal Tracking of Hematopoietic Stem and Progenitor Cells Marked by Five Fluorescent Proteins using Confocal and Multiphoton Microscopy

In vivo Clonal Tracking of Hematopoietic Stem and Progenitor Cells Marked by Five Fluorescent Proteins using Confocal and Multiphoton Microscopy

在造血干细胞和祖细胞利用共聚焦和多光子显微镜五荧光蛋白标记的活体克隆跟踪

We developed and validated a fluorescent marking methodology for clonal tracking of hematopoietic stem and progenitor cells (HSPCs) with high spatial and ...

Larval RNA Interference in the Red Flour Beetle, Tribolium castaneum

Larval RNA Interference in the Red Flour Beetle, Tribolium castaneum

幼虫RNA干扰在赤拟谷盗，<em&gt;赤拟谷盗</em

The red flour beetle, Tribolium&#160;castaneum, offers a repertoire of experimental tools for genetic and developmental studies, including a fully ...

Sperm Collection of Differential Quality Using Density Gradient Centrifugation

密度梯度离心法测定微分品质的精子

In sexual reproduction, a male gamete or sperm cell fuses with a female gamete to bring about fertilization. However, a large number of sperm cells with ...

Biological Sample Preparation by High-pressure Freezing, Microwave-assisted Contrast Enhancement, and Minimal Resin Embedding for Volume Imaging

高压冷冻、微波辅助对比度增强和最小树脂嵌入在体积成像中的生物样品制备

The described sample preparation technique is designed to combine the best quality of ultrastructural preservation with the most suitable contrast for the ...

Graphene Enclosure of Chemically Fixed Mammalian Cells for Liquid-Phase Electron Microscopy

用于液相电子显微镜的化学固定哺乳动物细胞的石墨烯外壳

A protocol is described for investigating the human epidermal growth factor receptor 2 (HER2) in the intact plasma membrane of breast cancer cells using ...

Cryo-Electron Microscopic Grid Preparation for Time-Resolved Studies using a Novel Robotic System, Spotiton

使用新型机器人系统 Spotiton 进行时间解决研究的低温电子微观网格准备

The capture of short-lived molecular states triggered by the early encounter of two or more interacting particles continues to be an experimental ...