January 2nd, 2013
•The cell membrane modification of red blood cells (RBCs) with hyperbranched polyglycerol (HPG) is presented. Modified RBCs were characterized by aqueous two phase partitioning, osmotic fragility and complement mediated lysis. The camouflage of surface proteins and antigens was evaluated using the flow cytometry and Micro Typing System (MTS) blood phenotyping cards.
Related Videos
Depletion of Specific Cell Populations by Complement Depletion
Isolation of Mouse Peritoneal Cavity Cells
Purification of Specific Cell Population by Fluorescence Activated Cell Sorting (FACS)
Expansion, Purification, and Functional Assessment of Human Peripheral Blood NK Cells
Methods for Quantitative Detection of Antibody-induced Complement Activation on Red Blood Cells
Real-time Imaging of Myeloid Cells Dynamics in ApcMin/+ Intestinal Tumors by Spinning Disk Confocal Microscopy
Detecting Glycogen in Peripheral Blood Mononuclear Cells with Periodic Acid Schiff Staining
Development of an Antigen-driven Colitis Model to Study Presentation of Antigens by Antigen Presenting Cells to T Cells
Using X-ray Crystallography, Biophysics, and Functional Assays to Determine the Mechanisms Governing T-cell Receptor Recognition of Cancer Antigens
Dextran Enhances the Lentiviral Transduction Efficiency of Murine and Human Primary NK Cells
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved