November 3rd, 2014
•In this protocol, we describe methods to efficiently transfect murine macrophage cell lines with siRNAs using the Amaxa Nucleofector 96-well Shuttle System, stimulate the macrophages with lipopolysaccharide, and monitor the effects on inflammatory cytokine production.
Related Videos
RNA Interference in Ticks (Video) | JoVE
Seven Steps to Stellate Cells (Video) | JoVE
An Introduction to Parasitic Wasps of Drosophila and the Antiparasite Immune Response Video (Video) | JoVE
Intravital Imaging of the Mouse Thymus using 2-Photon Microscopy Video (Video) | JoVE
Quantitative Measurement of the Immune Response and Sleep in Drosophila Video (Video) | JoVE
Quantification of the Respiratory Burst Response as an Indicator of Innate Immune Health in Zebrafish Video (Video) | JoVE
Dissecting Innate Immune Signaling in Viral Evasion of Cytokine Production Video (Video) | JoVE
In Vitro Analysis of Myd88-mediated Cellular Immune Response to West Nile Virus Mutant Strain Infection Video (Video) | JoVE
Non-invasive Imaging of the Innate Immune Response in a Zebrafish Larval Model of Streptococcus iniae Infection Video (Video) | JoVE
Two-photon Intravital Imaging of Leukocytes During the Immune Response in Lipopolysaccharide-treated Mouse Liver Video (Video) | JoVE
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved