December 6th, 2014
•This protocol describes the rapid non-enzymatic dissociation of fresh human tissue fragments for qualitative and quantitative assessment of CD45+ cells (lymphocytes/leukocytes) present in various normal and malignant human tissues. Additionally, the supernatant obtained from the primary tissue homogenate can be collected and stored for further analysis or experimentation.
Related Videos
Combination of Adhesive-tape-based Sampling and Fluorescence in situ Hybridization for Rapid Detection of Salmonella on Fresh Produce (Video) | JoVE
A Simple Chelex Protocol for DNA Extraction from Anopheles spp. (Video) | JoVE
A Simple Protocol for Platelet-mediated Clumping of Plasmodium falciparum-infected Erythrocytes in a Resource Poor Setting (Video) | JoVE
Rapid Analysis of Chromosome Aberrations in Mouse B Lymphocytes by PNA-FISH Video (Video) | JoVE
In Vitro Analysis of Myd88-mediated Cellular Immune Response to West Nile Virus Mutant Strain Infection Video (Video) | JoVE
Efficient Isolation Protocol for B and T Lymphocytes from Human Palatine Tonsils Video (Video) | JoVE
A Semi-automated Approach to Preparing Antibody Cocktails for Immunophenotypic Analysis of Human Peripheral Blood Video (Video) | JoVE
A RAPID Method for Blood Processing to Increase the Yield of Plasma Peptide Levels in Human Blood Video (Video) | JoVE
A Simple and Efficient Approach to Construct Mutant Vaccinia Virus Vectors Video (Video) | JoVE
Visualization of IL-22-expressing Lymphocytes Using Reporter Mice (Video) | JoVE
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved