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In Vitro Differentiation of Human CD4+FOXP3+ Induced Regulatory T Cells ITregs from Naïve CD4+ T Cells Using a TGF-β-containing Protocol Video (Video) | JoVE

DOI :

10.3791/55015-v

December 30th, 2016

December 30th, 2016

19,380 Views

1Unit of Computational Medicine, Center for Molecular Medicine, Department of Medicine Solna, Karolinska Institutet, Karolinska University Hospital, & Science for Life Laboratory

This protocol describes the reproducible generation and phenotyping of human induced regulatory T cells (iTregs) from naïve CD4+ T cells in vitro. Different protocols for FOXP3 induction allow for the study of specific iTreg phenotypes obtained with respective protocols.

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