blue-native-polyacrylamide-gel-electrophoresis-bn-page-for-analysis

Blue Native Polyacrylamide Gel Electrophoresis (BN-PAGE) for Analysis of Multiprotein Complexes from Cellular Lysates

Multiprotein complexes (MPCs) play a crucial role in cell signalling, since most proteins can be found in functional or regulatory complexes with other ...

University of Freiburg

Max-Planck-Institute of Immunology and Epigenetics

&#x5B66;&#x4E60;&#x5316;&#x5B66;&#x8BA1;&#x91CF;&#x5B66;&#x548C; T &#x7EC6;&#x80DE;&#x6297;&#x539F;&#x53D7;&#x4F53;&#x7684;&#x80DE;&#x5185;&#x8D29;&#x5356; (TCR) &#x662F;&#x7406;&#x89E3;&#x5176;&#x6D3B;&#x5316;&#x7684;&#x673A;&#x5236;&#x7684;&#x5173;&#x952E;&#x3002;AlphabetaTCR &#x5305;&#x62EC;&#x6297;&#x539F;&#x7ED3;&#x5408; TCRalphabeta &#x9ED1;&#x8179;&#x4EE5;&#x53CA;&#x6C19;&#x6C14; CD3epsilongamma&#x3001; CD3epsilondelta &#x548C; zeta2 &#x4E9A;&#x57FA;&#x7684;&#x4FE1;&#x53F7;&#x3002;&#x867D;&#x7136; TCR &#x76F8;&#x4E92;&#x4F5C;&#x7528;&#x5206;&#x5B50; (TRIM) &#x4E5F;&#x662F;&#x590D;&#x6742;&#x7684; alphabetaTCR &#x7684;&#x4E00;&#x90E8;&#x5206;&#xFF0C;&#x5B83;&#x5DF2;&#x4E0D;&#x88AB;&#x5217;&#x5165;&#x5927;&#x591A;&#x6570;&#x62A5;&#x544A;&#x4E3A;&#x6B62;&#x3002;

&#x5316;&#x5B66;&#x8BA1;&#x91CF;&#x5B66;&#x548C;&#x4FEE;&#x526A;&#x542B; TCR &#x914D;&#x5408;&#x7269;&#x7684;&#x7EC6;&#x80DE;&#x5185;&#x547D;&#x8FD0;&#x3002;

Studying the stoichiometry and intracellular trafficking of the T cell antigen receptor (TCR) is pivotal in understanding its mechanisms of activation. The alphabetaTCR includes the antigen-binding TCRalphabeta heterodimer as well as the signal transducing CD3epsilongamma, CD3epsilondelta and zeta2 subunits. Although the TCR-interacting molecule (TRIM) is also part of the alphabetaTCR complex, it has not been included in most reports so far.

Stoichiometry and intracellular fate of TRIM-containing TCR complexes.

&Eacute;tudier la stoechiom&eacute;trie et trafic intracellulaire du r&eacute;cepteur antig&egrave;ne des lymphocytes T (TCR) est essentiel dans la compr&eacute;hension de ses m&eacute;canismes d&#39;activation. L&#39;alphabetaTCR comprend l&#39;antig&egrave;ne-liant TCRalphabeta h&eacute;t&eacute;rodim&egrave;re ainsi que le signal transduction sous-unit&eacute;s CD3epsilongamma, CD3epsilondelta et zeta2. Bien que la mol&eacute;cule d&#39;interaction TCR (TRIM) fait &eacute;galement partie de l&#39;alphabetaTCR complexe, il n&#39;a pas &eacute;t&eacute; inclus dans la plupart des rapports jusqu&#39;ici.

Stoechiom&eacute;trie et intracellulaire sort des complexes contenant du TRIM TCR.

Studium der St&ouml;chiometrie und intrazellul&auml;re Handel mit den T-Zell-Antigen-Rezeptor ist (TCR) entscheidend f&uuml;r das Verst&auml;ndnis der Mechanismen der Aktivierung. Die AlphabetaTCR umfasst die Antigen-Bindung TCRalphabeta Heterodimer sowie das Signal transducing CD3epsilongamma, CD3epsilondelta und zeta2-Untereinheiten. Obwohl das TCR-Interaktion-Molek&uuml;l (TRIM) auch Bestandteil der komplexen AlphabetaTCR ist, ist es in den meisten Berichten nicht so weit enthalten.

St&ouml;chiometrie und intrazellul&auml;re Schicksal der TRIM-haltigen TCR-komplexe.

Studiare la stechiometria e traffico intracellulare del recettore dell&#39;antigene delle cellule T (TCR) &egrave; cruciale nella comprensione dei suoi meccanismi di attivazione. La alphabetaTCR include l&#39;eterodimero TCRalphabeta antigene-legante, come pure il segnale transducing subunit&agrave; CD3epsilongamma, CD3epsilondelta e zeta2. Anche se la molecola TCR-interacting (TRIM) &egrave; anche parte del complesso alphabetaTCR, esso non sia stato incluso nella maggior parte dei rapporti finora.

Stechiometria e destino intracellulare dei complessi contenenti TRIM TCR.

&#x5316;&#x5B66;&#x91CF;&#x8AD6;&#x3068; T &#x7D30;&#x80DE;&#x6297;&#x539F;&#x53D7;&#x5BB9;&#x4F53;&#x306E;&#x7D30;&#x80DE;&#x5185;&#x8F38;&#x9001;&#x3092;&#x52C9;&#x5F37; &#xFF08;TCR&#xFF09; &#x306F;&#x305D;&#x306E;&#x6D3B;&#x6027;&#x5316;&#x306E;&#x30E1;&#x30AB;&#x30CB;&#x30BA;&#x30E0;&#x3092;&#x7406;&#x89E3;&#x3059;&#x308B;&#x4E0A;&#x3067;&#x6975;&#x3081;&#x3066;&#x91CD;&#x8981;&#x3067;&#x3059;&#x3002;AlphabetaTCR &#x306B;&#x306F;&#x3001;&#x6297;&#x539F;&#x7D50;&#x5408; TCRalphabeta &#x30D8;&#x30C6;&#x30ED;&#x30C0;&#x30A4;&#x30DE;&#x30FC;&#x306B;&#x306F; CD3epsilongamma&#x3001;CD3epsilondelta&#x3001;zeta2 &#x306E;&#x30B5;&#x30D6;&#x30E6;&#x30CB;&#x30C3;&#x30C8;&#x3092;&#x4F1D;&#x9054;&#x4FE1;&#x53F7;&#x304C;&#x542B;&#x307E;&#x308C;&#x307E;&#x3059;&#x3002;TCR &#x306E;&#x76F8;&#x4E92;&#x4F5C;&#x7528;&#x306E;&#x5206;&#x5B50; &#xFF08;&#x30C8;&#x30EA;&#x30E0;&#xFF09; &#x3082;&#x8907;&#x96D1;&#x306A; alphabetaTCR &#x306E;&#x4E00;&#x90E8;&#x3067;&#x3059;&#x304C;&#x3001;&#x305D;&#x308C;&#x307B;&#x3068;&#x3093;&#x3069;&#x306E;&#x30EC;&#x30DD;&#x30FC;&#x30C8;&#x3067;&#x3053;&#x308C;&#x307E;&#x3067;&#x542B;&#x307E;&#x308C;&#x3066;&#x3044;&#x307E;&#x305B;&#x3093;&#x3002;

&#x5316;&#x5B66;&#x91CF;&#x8AD6;&#x3068;&#x30C8;&#x30EA;&#x30E0;&#x3092;&#x542B;&#x3080; TCR &#x8907;&#x5408;&#x4F53;&#x306E;&#x7D30;&#x80DE;&#x306E;&#x904B;&#x547D;&#x3002;

Stoichiometry &#xBC0F; T &#xC138;&#xD3EC; &#xD56D; &#xC6D0; &#xC218;&#xC6A9; &#xCCB4;&#xC758; &#xC138;&#xD3EC;&#xB0B4; &#xBC00;&#xB9E4; &#xACF5;&#xBD80; (TCR) &#xD65C;&#xC131;&#xD654;&#xC758; &#xADF8;&#xAC83;&#xC758; &#xBA54;&#xCEE4;&#xB2C8;&#xC998;&#xC744; &#xC774;&#xD574;&#xC5D0; &#xD575;&#xC2EC; &#xC774;&#xB2E4;. Alphabetatcr&#xC5D0; antigen &#xBC14;&#xC778;&#xB529; TCRalphabeta heterodimer transducing CD3epsilongamma, CD3epsilondelta &#xBC0F; zeta2 &#xD558;&#xC704; &#xB2E8;&#xC704; &#xC2E0;&#xD638; &#xD3EC;&#xD568; &#xB429;&#xB2C8;&#xB2E4;. TCR &#xC791;&#xC6A9; &#xBD84;&#xC790; (&#xD2B8;&#xB9BC;) &#xB610;&#xD55C; &#xBCF5;&#xC7A1; &#xD55C; Alphabetatcr&#xC758; &#xC77C;&#xBD80; &#xC774;&#xC9C0;&#xB9CC;, &#xADF8;&#xAC83;&#xC740; &#xD3EC;&#xD568; &#xB418;&#xC9C0; &#xB300;&#xBD80;&#xBD84;&#xC758; &#xBCF4;&#xACE0;&#xC11C;&#xC5D0;&#xC11C; &#xC9C0;&#xAE08;&#xAE4C;&#xC9C0;.

Stoichiometry &#xBC0F; &#xD2B8;&#xB9BC; &#xD3EC;&#xD568; &#xB41C; TCR &#xB2E8;&#xC9C0;&#xC758; &#xC138;&#xD3EC;&#xB0B4; &#xC6B4;&#xBA85;.

Estudio de la estequiometr&iacute;a y tr&aacute;fico intracelular del receptor del ant&iacute;geno de c&eacute;lula T (TCR) es fundamental en la comprensi&oacute;n de sus mecanismos de activaci&oacute;n. El alphabetaTCR incluye el heterod&iacute;mero de TCRalphabeta uni&oacute;n ant&iacute;geno as&iacute; como la se&ntilde;al de transducci&oacute;n de subunidades CD3epsilongamma, CD3epsilondelta y zeta2. Aunque la mol&eacute;cula de interacci&oacute;n TCR (TRIM) tambi&eacute;n forma parte de la alphabetaTCR complejo, ha no ha incluido en la mayor&iacute;a de los informes hasta ahora.

Gina J. Fiala

Spemann Graduate School of Biology and Medicine (SGBM),
Centre for Biological Signalling Studies (bioss) and Biology III,
Faculty of Biology,
Centre for Biological Signalling Studies (bioss) and Biology III, Faculty of Biology

University of Freiburg

Blue Native Polyacrylamide Gel Electrophoresis (BN-PAGE) for Analysis of Multiprotein Complexes from Cellular Lysates

Gina J. Fiala

Spemann Graduate School of Biology and Medicine (SGBM),Centre for Biological Signalling Studies (bioss) and Biology III,Faculty of Biology,Centre for Biological Signalling Studies (bioss) and Biology III, Faculty of Biology

University of Freiburg

Blue Native Polyacrylamide Gel Electrophoresis (BN-PAGE) for Analysis of Multiprotein Complexes from Cellular Lysates

Spemann Graduate School of Biology and Medicine (SGBM),
Centre for Biological Signalling Studies (bioss) and Biology III,
Faculty of Biology,
Centre for Biological Signalling Studies (bioss) and Biology III, Faculty of Biology