automated-analysis-nematode-population-based-chemosensory-preference

Automated Analysis of a Nematode Population-based Chemosensory Preference Assay

The nematode, Caenorhabditis elegans' compact nervous system of only 302 neurons underlies a diverse repertoire of behaviors. To facilitate the dissection ...

California Institute of Technology

The GAL4-UAS system is a powerful tool for manipulating gene expression, but its application in Caenorhabditis elegans has not been described. Here we systematically optimize the system's three main components to develop a temperature-optimized GAL4-UAS system (cGAL) that robustly controls gene expression in C. elegans from 15 to 25 °C. We demonstrate this system's utility in transcriptional reporter analysis, site-of-action experiments and exogenous transgene expression; and we provide a basic driver and effector toolkit.

Cynthia M. Chai

Division of Biology and Bioengineering,
Howard Hughes Medical Institute

California Institute of Technology

Automated Analysis of a Nematode Population-based Chemosensory Preference Assay

Cynthia M. Chai

.css-o250i3{font-size:18px;font-family:Open Sans,sans-serif;line-height:21.6px;}Division of Biology and Bioengineering,Howard Hughes Medical Institute

California Institute of Technology

Automated Analysis of a Nematode Population-based Chemosensory Preference Assay

Division of Biology and Bioengineering,
Howard Hughes Medical Institute