application-intelligent-high-throughput-antimicrobial-sensitivity

Application of the Intelligent High-Throughput Antimicrobial Sensitivity Testing/Phage Screening System and Lar Index of Antimicrobial Resistance

To improve the efficiency of antimicrobial susceptibility testing (AST) and phage high-throughput screening for resistant bacteria and to reduce the ...

Key Laboratory of Livestock and Poultry Multi-omics of MARA

 is an important zoonotic pathogen that poses a serious threat to the pig industry and human health. The massive use of macrolides has led to the emergence of resistance in , and  is suspected to be a reservoir of antimicrobial resistance genes. The mechanism to macrolide resistance in  is mainly due to  and . In this study, loop-mediated isothermal amplification (LAMP) methods were developed to detect  and  genes in  through turbidimetry detection. The sensitivity and specificity of the LAMP reactions were determined. All results of LAMP and polymerase chain reaction (PCR) assay were compared to determine whether LAMP method was accurate and reliable. The results showed that all 100 nonstreptococcus clinical isolates tested negative, indicating the high specificity of LAMP assays. The detection limit of LAMP assay was 1 fg per reaction, and 10-10-fold lower than those of conventional PCR methods. Evaluation of the performance of the LAMP assay in  clinical strains revealed a good consistency between LAMP and PCR assays. In conclusion, LAMP assays are specific, sensitive, and rapid methods to detect  and  in .

Xiaonan Zhao

Key Laboratory of Livestock and Poultry Multi-omics of MARA

Application of the Intelligent High-Throughput Antimicrobial Sensitivity Testing/Phage Screening System and Lar Index of Antimicrobial Resistance