Renji Hospital, Shanghai Jiaotong University, School of Medicine

1 ARTICLES PUBLISHED IN JoVE

Neuroscience

Extraction and Tissue Clearing Preparation of Mouse Brain-Spinal Cord Samples

Xiaoxin Zhou^*1,2,3, Zhixing Lu^*1,2, Wanbing Dai^*1,2, Xiaoyu Sun^*1,2

¹Department of Anesthesiology, Renji Hospital, Shanghai Jiaotong University, School of Medicine, ²Key Laboratory of Anesthesiology, (Shanghai Jiaotong University), Ministry of Education, ³Department of Anesthesiology, Ruijin Hospital, Shanghai Jiaotong University, School of Medicine

This study presents a protocol for extracting and preparing cleared whole-brain and spinal cord samples with preserved fluorescent signals, enhancing experimental efficiency and data integrity to advance neuroscience research.