Live-Cell Förster Resonance Energy Transfer Imaging of Metabolically Regulated Akt Activation Dynamics in HepG2 CellsJaved Akhtar 1,2,3,4,5, Muhammad Imran 6, Jiahe Wang 1,2,3, Gang Ma 5, Guanyu Wang 1,2,3
1Laboratory of Biocomplexity and Engineering Biology, School of Medicine, The Chinese University of Hong Kong, 2Futian Biomedical Innovation R&D Center, The Chinese University of Hong Kong, 3Ciechanover Institute of Precision and Regenerative Medicine, School of Medicine, The Chinese University of Hong Kong, 4Department of Immunology and Microbiology, School of Life Sciences, Southern University of Science and Technology, 5Department of Biology, School of Life Sciences, Southern University of Science and Technology, 6Department of Computer Science and IT, University of Southern Punjab
Here, we present a protocol to quantify the spatiotemporal dynamics of Akt activation and phosphorylation in live HepG2 cells. Förster resonance energy transfer (FRET) imaging is a powerful tool that provides valuable insights into insulin signaling pathways and metabolic regulation in cancer cells.
