India Ink Inflation: A Staining Method to Visualize Tumor Nodules

Protocol

1. Validation of Lung Metastases Using India Ink Inflation of Tumor-bearing Lungs

NOTE: To validate the luciferase live tumor imaging results, perform India ink inflation of tumor-bearing mouse lungs to quantify tumor nodules. 4T1 cells also metastasize to other organs and tissues (Figure 1) and therefore, histological examination of these organs to validate tumor metastasis should be performed if needed. This protocol use lung metastasis as a example.

1. Keep mice in their existing home cage for euthanasia. When housed with other animals, relocate those mice not being euthanized t....

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النتائج

Figure 1. Visualization of Lung Tumor Nodules by India Ink Inflation. The lung of the tumor-bearing mouse was inflated with India ink and fixed in Fekete's solution. The white dots are lung metastases.

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Materials

Name	Company	Catalog Number	Comments
9 mm AutoClip Applier	Becton Dickinson Primary Care Diagnostics. Sparks, MD	427630
Sharp-Pointed Dissecting Scissors	Fisher	8940
Dissecting Fine-Pointed Forceps	Fisher	8875
70% Ethanol	Ultrapure-usa.com
1/2 CC 27 G 1/2 tuberculin syringe	Becton Dickinson and Co. NJ	305620