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This video describes an in vivo method to screen the protective potential of neuroprotective test compounds in reducing polyQ aggregation in body wall muscle cells of nematodes and ameliorating aggregation-associated proteotoxicity.
1. PolyQ aggregation assay
Name | Company | Catalog Number | Comments |
C. elegans strain | |||
AM141 rmIs133 [unc-54p::Q40::YFP] | Caenorhabditis Genetics Center (CGC) | https://cgc.umn.edu/strain/AM141 | |
E. coli strain | |||
OP50 | Caenorhabditis Genetics Center (CGC) | https://cgc.umn.edu/strain/OP50 | |
Reagent | |||
Sodium azide | Sinopharm Chemical Reagent Co., Ltd. | 80115560 | https://www.reagent.com.cn/goodsDetail/5e981aa807664e26af 551e96ff5f07cd |
Equipment | |||
384-well cell culture plate | Nest Biotechnology Co., Ltd. | 761001 | https://www.cell-nest.com/page94? _l=en&product_id=85 |
48-well cell culture plate | Nest Biotechnology Co., Ltd. | 748001 | https://www.cell-nest.com/page94? _l=en&product_id=85 |
Fluorescence microscope | Guangzhou Micro-shot Optical Technology Co., Ltd. | Mshot MF31-LED | https://www.mshot.com/ article/442.html |
High-content imaging system | Molecular Devices | ImageXpress | https://www.moleculardevices.com/ products/cellular-imagingsystems#High-Content-Imaging |
Microcentrifuge | GeneCompany | GENESPEED X1 | https://www.genecompany.com/ index.php/Home/Goods/ goodsdetails/gid/189.html |
Parafilm M | Sigma-Aldrich | P7793-1EA | https://www.sigmaaldrich.cn/CN/en/product/sigma/p7793?context=product |
Microscope digital camera | Guangzhou Micro-shot Optical Technology Co., Ltd. | MS60 | https://www.mshot.com/article/677.html |
Software | |||
Image acquisition and analysis software | Molecular Devices | MetaXpress | https://www.moleculardevices.com/ products/cellular-imaging-systems/ acquisition-and-analysis-software/ metaxpress |
This article has been published
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Source: Wang, Q. et al. Caenorhabditis elegans as a Model System for Discovering Bioactive Compounds Against Polyglutamine-Mediated Neurotoxicity. J. Vis. Exp. (2021)
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