وأيد هذا العمل عن طريق المنح من قبل مؤسسة ماذرز والمعاهد الوطنية للصحة (منح عدد 5P50NS019632 - 27) وانطونيو لDamasio حنا.

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T., Essex, R., Webber, C., Damasio, H., Damasio, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Predicting+visual+stimuli+on+the+basis+of+activity+in+auditory+cortices.">Predicting visual stimuli on the basis of activity in auditory cortices.</a> Nat. Neurosci. 13, 667-668 (2010).</li><li>Meyer, K., Kaplan, J. T., Essex, R., Damasio, H., Damasio, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Seeing+touch+is+correlated+with+content-specific+activity+in+primary+somatosensory+cortex.">Seeing touch is correlated with content-specific activity in primary somatosensory cortex.</a> Cereb. Cortex. 21, 2113-2121 (2011).</li><li>Damasio, A. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Time-locked+multiregional+retroactivation:+a+systems-level+proposal+for+the+neural+substrates+of+recall+and+recognition.">Time-locked multiregional retroactivation: a systems-level proposal for the neural substrates of recall and recognition.</a> Cognition. 33, 25-62 (1989).</li><li>Meyer, K., Damasio, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Convergence+and+divergence+in+a+neural+architecture+for+recognition+and+memory.">Convergence and divergence in a neural architecture for recognition and memory.</a> Trends. Neurosci. 32, 376-382 (2009).</li><li>Friston, K. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Modalities,+modes,+and+models+in+functional+neuroimaging.">Modalities, modes, and models in functional neuroimaging.</a> Science. 326, 399-403 (2009).</li><li>Hanke, M., Halchenko, Y. O., Sederberg, P. B., Hanson, S. J., Haxby, J. V., Pollmann, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=PyMVPA:+a+Python+toolbox+for+multivariate+pattern+analysis+of+fMRI+data.">PyMVPA: a Python toolbox for multivariate pattern analysis of fMRI data.</a> Neuroinformatics. 7, 37-53 (2009).</li><li>Harrison, S. A., Tong, F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Decoding+reveals+the+contents+of+visual+working+memory+in+early+visual+areas.">Decoding reveals the contents of visual working memory in early visual areas.</a> Nature. 458, 632-635 (2009).</li><li>Serences, J. T., Ester, E. F., Vogel, E. K., Awh, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Stimulus-specific+delay+activity+in+human+primary+visual+cortex.">Stimulus-specific delay activity in human primary visual cortex.</a> Psych Sci. 20, 207-214 (2009).</li></ol>

الإعلان عن أي تضارب في المصالح.

نتائج دراساتنا السابقة تثبت أن نشاطهم البدني عنهن بنحو مشروط عبر أداة مفيدة لدراسة يرتبط العصبية من الصور العقلية التي تعرضت في &quot;أذن العقل&quot; و &quot;العقل اللمس&quot;. على وجه التحديد ، فقد بينت النتائج أن يرتبط مضمون مثل هذه الصور مع النشاط العصبي في السمع في وقت مبكر والقشور الحسية الجسدية ، على التوالي ، وتقديم الدعم المباشر للإطار التجريبية Damasio من التقارب اختلاف المناطق. ويمكن تمديد النموذج الأساسي وصفنا في عدد من الطرق. الأكثر وضوحا ، ويمكن إجراء دراسات مماثلة باستخدام تركيبات مختلفة من طرائق الحسية. في هذا الصدد ، فمن الممكن أن إنشاء بعض الجمعيات عبر مشروط قبل التجربة قد يزيد من فرص النجاح. على سبيل المثال ، لدراسة التمثيل مشروط عبر حاسة الشم في القشور ، يمكن للمرء أن الموضوعات التي يعرضها رئيس الوزراء في الوقت نفسه إلى البصر والشم لعدد من المواد الغذائية. Shortly بعد ، داخل الماسح الرنين المغناطيسي الوظيفي ، سيكون أثار ذكريات شمية من الروائح التي منبهات البصرية ، ويمكن استخدامها في نشاطهم البدني عنهن بنحو محاولة لتعيين المحاكمات المواد الغذائية الصحيحة التي تستند فقط على النشاط في قشرات الشمية. سؤال آخر للاهتمام هو ما إذا كانت أنماط عبر modally بفعل النشاط في القشور الحسية في وقت مبكر تتحمل تشابه مع الأنماط التي يسببها عندما تتوافر لديها الخبرة في الواقع الصوت أو اللمس ، وبعبارة أخرى ، لا نرى إناء من الزجاج استدعاء تتحطم نمطا مماثلا لل النشاط العصبي في وقت مبكر قشرات السمعية فعلا الاستماع الى الحدث نفسه؟ هذا السؤال ، مرة أخرى ، يمكن معالجتها من خلال نشاطهم البدني عنهن بنحو : هو المصنف الذي تم تدريبهم على البيانات المسجلة في حين سمع دوي بعض الموضوعات قادرة على التمييز بشكل صحيح البيانات المسجلة في حين أن الموضوعات شاهدت لقطات فيديو المقابلة؟ في دراسة السمع لدينا ، ولم نحاول مثل هذا التصنيف ، ولكن النتائج كانت هامشية (انظر الشكل 3 في المرجع 7). في تلك الدراسة ، ومع ذلك ، لم تكن تسيطر على الجمعيات السمعية من المشاركين بأي شكل من الأشكال ، وبعبارة أخرى ، نحن لا نعرف كيف مماثلة في كل مادة سمعية الصور المرتبطة أشرطة الفيديو وعلى المسارات الصوتية المستخدمة لتدريب المصنف. مرة أخرى ، قد يكون من المثير للاهتمام أن معالجة مسألة واحدة بعد أن تم تجهيزهم محددة عبر مشروط الجمعيات في الموضوعات ، كما هو موضح أعلاه للجمعية visuo - الشمية. هذا من شأنه أن يسمح للسيطرة على مزيد من الخبرة العقلية موثوق من الموضوعات خلال المحاكمات ، وبالتالي قد الفيديو زيادة أداء التنبؤ مصنف. على أن تبرم ، أدخلنا امتدادا لنشاطهم البدني عنهن بنحو النموذج الكلاسيكي من خلال إظهار أنه يمكن التنبؤ ليس فقط داخل المحفزات ، ولكن أيضا عبر طرائق الحسية. وهكذا ، وتبين لنا أن لا يقتصر على استخدام لنشاطهم البدني عنهن بنحو يرتبط بالتحقيق في التمثيل الإدراكي الناجم مباشرة عن طريق الحواس الخارجية. بدلا من ذلك ، يمكن أيضا نشاطهم البدني عنهن بنحوتقييم الركيزة العصبية من الصور الذهنية التي يتم تشغيلها داخليا : وفقا للإطار Damasio في منطقة التقارب الاختلاف ، النتائج التي توصلنا إليها تشير إلى أن يرتبط تجربة واعية من الصور الذهنية التي يتم بناؤها على أساس ذكريات مع محتوى محددة تمثيلات العصبية في الحسية في وقت مبكر قشرات.

cross-modal multivariate pattern analysis

التحليل متعدد المتغيرات نمط (نشاطهم البدني عنهن بنحو) هو الأسلوب شعبية متزايدة لتحليل التصوير بالرنين المغناطيسي الوظيفي (fMRI) البيانات 1-4. عادة ، يتم استخدام طريقة للتعرف على التجربة الحسية للموضوع من النشاط العصبي في مناطق معينة من المخ. على سبيل المثال ، استخدمت لتوقع اتجاه حواجز شبكية بصرية موضوعا ترى من النشاط في وقت مبكر 5 قشرات البصرية ، أو بالقياس ، ومضمون خطاب من النشاط في وقت مبكر 6 قشرات السمعية. هنا ، نقدم امتدادا لنشاطهم البدني عنهن بنحو النموذج الكلاسيكي ، وفقا لوالتي لا يتوقع المحفزات الحسية داخل ، ولكن عبر الأنظمة الحسية. على وجه التحديد ، الأسلوب وصفنا يتناول مسألة ما إذا كانت المحفزات التي تثير جمعيات الذاكرة في طرائق أخرى غير تلك التي يتم من خلالها تقديم هذه لحث أنماط النشاط محتوى معين في القشور الحسية تلك الطرائق الأخرى.على سبيل المثال ، رؤية مقطع فيديو صامت من إناء الزجاج تحطم على أرض الواقع ، بصورة تلقائية ، في معظم المراقبين صورة السمعي للصوت المرتبطة بها ؛ هي تجربة هذه الصورة في &quot;أذن العقل&quot; ويرتبط مع نمط النشاط العصبي محددة في وقت مبكر قشرات السمعي؟ وعلاوة على ذلك ، وهذا هو نمط النشاط متميزة من النمط الذي يمكن ملاحظة إذا كان هذا الموضوع ، بدلا من ذلك ، ومشاهدة شريط فيديو لكلب يعوي؟ في دراستين 7،8 السابقة ، كنا قادرين على التنبؤ الصوت واللمس مما يعني مقاطع الفيديو التي تعتمد على النشاط العصبي في السمع في وقت مبكر والقشور الحسية الجسدية ، على التوالي. نتائجنا تتفق مع الإطار الذي اقترحه neuroarchitectural Damasio 9،10 ، وفقا للتجربة التي الصور الذهنية التي تستند إلى ذكريات -- مثل سماع صوت تحطيم مزهرية في &quot;أذن العقل&quot; وعند رؤية شريط الفيديو المقابلة معتمد من قبل إعادة بناء المحتوى -- مقطعأنماط محددة النشاط العصبي في القشور الحسية في وقت مبكر.

Watch this Scientific Journal Video about Cross-Modal Multivariate Pattern Analysis at JoVE.com

Cross-Modal Multivariate Pattern Analysis

تحليل نمط الكلاسيكي المتعدد يتوقع المحفزات الحسية موضوعا ترى من النشاط العصبي في قشرات المقابلة (على سبيل المثال من المثيرات البصرية النشاط في القشرة البصرية). هنا ، ونحن نطبق تحليل نمط عبر modally وتبين أن الصوت واللمس مما يعني المثيرات البصرية ويمكن التنبؤ به من نشاط في السمعية والحسية الجسدية القشور ، على التوالي.

عبر تحليل نمط مشروط متعدد المتغيرات

Multivariate pattern analysis (MVPA) is an increasingly popular method of analyzing functional magnetic resonance imaging (fMRI) data1-4. Typically, the ...

cross-modal-multivariate-pattern-analysis

Research

JoVE Journal

Neuroscience

19.8K Views.  University of Southern California. تحليل نمط الكلاسيكي المتعدد يتوقع المحفزات الحسية موضوعا ترى من النشاط العصبي في قشرات المقابلة (على سبيل المثال من المثيرات البصرية النشاط في القشرة البصرية). هنا ، ونحن نطبق تحليل نمط عبر modally وتبين أن الصوت واللمس مما يعني المثيرات البصرية ويمكن التنبؤ به من ?...

Video: Cross-Modal Multivariate Pattern Analysis

Basics of Multivariate Analysis in Neuroimaging Data

Multivariate analysis techniques for neuroimaging data have recently received increasing attention as they have many attractive features that cannot be easily realized by the more commonly used univariate, voxel-wise, techniques1,5,6,7,8,9. Multivariate approaches evaluate correlation/covariance of activation across brain regions, rather than proceeding on a voxel-by-voxel basis. Thus, their results can be more easily interpreted as a signature of neural networks. Univariate approaches, on the other hand, cannot directly address interregional correlation in the brain. Multivariate approaches can also result in greater statistical power when compared with univariate techniques, which are forced to employ very stringent corrections for voxel-wise multiple comparisons. Further, multivariate techniques also lend themselves much better to prospective application of results from the analysis of one dataset to entirely new datasets. Multivariate techniques are thus well placed to provide information about mean differences and correlations with behavior, similarly to univariate approaches, with potentially greater statistical power and better reproducibility checks. In contrast to these advantages is the high barrier of entry to the use of multivariate approaches, preventing more widespread application in the community. To the neuroscientist becoming familiar with multivariate analysis techniques, an initial survey of the field might present a bewildering variety of approaches that, although algorithmically similar, are presented with different emphases, typically by people with mathematics backgrounds. We believe that multivariate analysis techniques have sufficient potential to warrant better dissemination. Researchers should be able to employ them in an informed and accessible manner. The current article is an attempt at a didactic introduction of multivariate techniques for the novice. A conceptual introduction is followed with a very simple application to a diagnostic data set from the Alzheimer s Disease Neuroimaging Initiative (ADNI), clearly demonstrating the superior performance of the multivariate approach.

The current article describes the basics of multivariate analysis and contrasts it to the more commonly used voxel-wise univariate analysis. Both types of analysis are applied to a clinical-neuroscience data set. Supplementary split-half simulations show better replication of the multivariate results in independent data sets.

أساسيات التحليل متعدد المتغيرات في البيانات تصوير الأعصاب

Multivariate analysis techniques for neuroimaging data have recently received increasing attention as they have many attractive features that cannot be ...

T-maze Forced Alternation and Left-right Discrimination Tasks for Assessing Working and Reference Memory in Mice

Forced alternation and left-right discrimination tasks using the T-maze have been widely used to assess working and reference memory, respectively, in rodents. In our laboratory, we evaluated the two types of memory in more than 30 strains of genetically engineered mice using the automated version of this apparatus. Here, we present the modified T-maze apparatus operated by a computer with a video-tracking system and our protocols in a movie format. The T-maze apparatus consists of runways partitioned off by sliding doors that can automatically open downward, each with a start box, a T-shaped alley, two boxes with automatic pellet dispensers at one side of the box, and two L-shaped alleys. Each L-shaped alley is connected to the start box so that mice can return to the start box, which excludes the effects of experimenter handling on mouse behavior. This apparatus also has an advantage that in vivo microdialysis, in vivo electrophysiology, and optogenetics techniques can be performed during T-maze performance because the doors are designed to go down into the floor. In this movie article, we describe T-maze tasks using the automated apparatus and the T-maze performance of &alpha;-CaMKII+/- mice, which are reported to show working memory deficits in the eight-arm radial maze task. Our data indicated that &alpha;-CaMKII+/- mice showed a working memory deficit, but no impairment of reference memory, and are consistent with previous findings using the eight-arm radial maze task, which supports the validity of our protocol. In addition, our data indicate that mutants tended to exhibit reversal learning deficits, suggesting that &alpha;-CaMKII deficiency causes reduced behavioral flexibility. Thus, the T-maze test using the modified automatic apparatus is useful for assessing working and reference memory and behavioral flexibility in mice.

This article presents the protocol of T-maze tests using a modified automated apparatus for assessing the learning and memory functions in mice.

T-المتاهة التناوب القسري والمهام التمييز بين اليسار واليمين لتقييم العمل ومرجع الذاكرة في الفئران

Forced alternation and left-right discrimination tasks using the T-maze have been widely used to assess working and reference memory, respectively, in ...

Creating Objects and Object Categories for Studying Perception and Perceptual Learning

In order to quantitatively study object perception, be it perception by biological systems or by machines, one needs to create objects and object categories with precisely definable, preferably naturalistic, properties1. Furthermore, for studies on perceptual learning, it is useful to create novel objects and object categories (or object classes) with such properties2.
Many innovative and useful methods currently exist for creating novel objects and object categories3-6 (also see refs. 7,8). However, generally speaking, the existing methods have three broad types of shortcomings.
First, shape variations are generally imposed by the experimenter5,9,10, and may therefore be different from the variability in natural categories, and optimized for a particular recognition algorithm. It would be desirable to have the variations arise independently of the externally imposed constraints.
Second, the existing methods have difficulty capturing the shape complexity of natural objects11-13. If the goal is to study natural object perception, it is desirable for objects and object categories to be naturalistic, so as to avoid possible confounds and special cases.
Third, it is generally hard to quantitatively measure the available information in the stimuli created by conventional methods. It would be desirable to create objects and object categories where the available information can be precisely measured and, where necessary, systematically manipulated (or 'tuned'). This allows one to formulate the underlying object recognition tasks in quantitative terms.
Here we describe a set of algorithms, or methods, that meet all three of the above criteria. Virtual morphogenesis (VM) creates novel, naturalistic virtual 3-D objects called 'digital embryos' by simulating the biological process of embryogenesis14. Virtual phylogenesis (VP) creates novel, naturalistic object categories by simulating the evolutionary process of natural selection9,12,13. Objects and object categories created by these simulations can be further manipulated by various morphing methods to generate systematic variations of shape characteristics15,16. The VP and morphing methods can also be applied, in principle, to novel virtual objects other than digital embryos, or to virtual versions of real-world objects9,13. Virtual objects created in this fashion can be rendered as visual images using a conventional graphical toolkit, with desired manipulations of surface texture, illumination, size, viewpoint and background. The virtual objects can also be 'printed' as haptic objects using a conventional 3-D prototyper.
We also describe some implementations of these computational algorithms to help illustrate the potential utility of the algorithms. It is important to distinguish the algorithms from their implementations. The implementations are demonstrations offered solely as a 'proof of principle' of the underlying algorithms. It is important to note that, in general, an implementation of a computational algorithm often has limitations that the algorithm itself does not have.
Together, these methods represent a set of powerful and flexible tools for studying object recognition and perceptual learning by biological and computational systems alike. With appropriate extensions, these methods may also prove useful in the study of morphogenesis and phylogenesis.

We describe a novel methodology for creating naturalistic 3-D objects and object categories with precisely defined feature variations. We use simulations of the biological processes of morphogenesis and phylogenesis to create novel, naturalistic virtual 3-D objects and object categories that can then be rendered as visual images or haptic objects.

إنشاء كائنات وفئات كائن لدراسة الإدراك الحسي والتعلم

In order to quantitatively study object perception, be it perception by biological systems or by machines, one needs to create objects and object ...

Visualization and Genetic Manipulation of Dendrites and Spines in the Mouse Cerebral Cortex and Hippocampus using In utero Electroporation

In utero electroporation (IUE) has become a powerful technique to study the development of different regions of the embryonic nervous system 1-5. To date this tool has been widely used to study the regulation of cellular proliferation, differentiation and neuronal migration especially in the developing cerebral cortex 6-8. Here we detail our protocol to electroporate in utero the cerebral cortex and the hippocampus and provide evidence that this approach can be used to study dendrites and spines in these two cerebral regions.
Visualization and manipulation of neurons in primary cultures have contributed to a better understanding of the processes involved in dendrite, spine and synapse development. However neurons growing in vitro are not exposed to all the physiological cues that can affect dendrite and/or spine formation and maintenance during normal development. Our knowledge of dendrite and spine structures in vivo in wild-type or mutant mice comes mostly from observations using the Golgi-Cox method 9. However, Golgi staining is considered to be unpredictable. Indeed, groups of nerve cells and fiber tracts are labeled randomly, with particular areas often appearing completely stained while adjacent areas are devoid of staining. Recent studies have shown that IUE of fluorescent constructs represents an attractive alternative method to study dendrites, spines as well as synapses in mutant / wild-type mice 10-11 (Figure 1A). Moreover in comparison to the generation of mouse knockouts, IUE represents a rapid approach to perform gain and loss of function studies in specific population of cells during a specific time window. In addition, IUE has been successfully used with inducible gene expression or inducible RNAi approaches to refine the temporal control over the expression of a gene or shRNA 12. These advantages of IUE have thus opened new dimensions to study the effect of gene expression/suppression on dendrites and spines not only in specific cerebral structures (Figure 1B) but also at a specific time point of development (Figure 1C).
Finally, IUE provides a useful tool to identify functional interactions between genes involved in dendrite, spine and/or synapse development. Indeed, in contrast to other gene transfer methods such as virus, it is straightforward to combine multiple RNAi or transgenes in the same population of cells. 
In summary, IUE is a powerful method that has already contributed to the characterization of molecular mechanisms underlying brain function and disease and it should also be useful in the study of dendrites and spines.

Visualization and Genetic Manipulation of Dendrites and Spines in the Mouse Cerebral Cortex and Hippocampus using In utero Electroporation

This article describes in detail a protocol to electroporate in utero the cerebral cortex and the hippocampus at E14.5 in mice. We also show that this is a valuable method to study dendrites and spines in these two cerebral regions.

التصور والتلاعب الجيني من التشعبات والاعمده الفقريه في قشرة الدماغ والحصين ماوس باستخدام<em&gt; في الرحم</em&gt; Electroporation

In utero electroporation (IUE) has become a powerful technique to study the development of different regions of the embryonic nervous system 1-5. To date ...

The Swimmeret System of Crayfish: A Practical Guide for the Dissection of the Nerve Cord and Extracellular Recordings of the Motor Pattern

Here we demonstrate the dissection of the crayfish abdominal nerve cord. The preparation comprises the last two thoracic ganglia (T4, T5) and the chain of abdominal ganglia (A1 to A6). This chain of ganglia includes the part of the central nervous system (CNS) that drives coordinated locomotion of the pleopods (swimmerets): the swimmeret system. It is known for over five decades that in crayfish each swimmeret is driven by its own independent pattern generating kernel that generates rhythmic alternating activity 1-3. The motor neurons innervating the musculature of each swimmeret comprise two anatomically and functionally distinct populations 4. One is responsible for the retraction (power stroke, PS) of the swimmeret. The other drives the protraction (return stroke, RS) of the swimmeret. Motor neurons of the swimmeret system are able to produce spontaneously a fictive motor pattern, which is identical to the pattern recorded in vivo 1.
The aim of this report is to introduce an interesting and convenient model system for studying rhythm generating networks and coordination of independent microcircuits for students&#8217; practical laboratory courses. The protocol provided includes step-by-step instructions for the dissection of the crayfish&#8217;s abdominal nerve cord, pinning of the isolated chain of ganglia, desheathing the ganglia and recording the swimmerets fictive motor pattern extracellularly from the isolated nervous system.
Additionally, we can monitor the activity of swimmeret neurons recorded intracellularly from dendrites. Here we also describe briefly these techniques and provide some examples. Furthermore, the morphology of swimmeret neurons can be assessed using various staining techniques. Here we provide examples of intracellular (by iontophoresis) dye filled neurons and backfills of pools of swimmeret motor neurons. In our lab we use this preparation to study basic functions of fictive locomotion, the effect of sensory feedback on the activity of the CNS, and coordination between microcircuits on a cellular level.

Here we describe the dissection of the crayfish abdominal nerve cord. We also demonstrate an electrophysiological technique to record fictive locomotion from swimmeret motor neurons.

نظام Swimmeret من جراد البحر: دليل عملي للتشريح من الحبل العصب وخارج الخلية تسجيلات من نمط للسيارات

Here we demonstrate the dissection of the crayfish abdominal nerve cord. The preparation comprises the last two thoracic ganglia (T4, T5) and the chain of ...

Peering at Brain Polysomes with Atomic Force Microscopy

The translational machinery, i.e., the polysome or polyribosome, is one of the biggest and most complex cytoplasmic machineries in cells. Polysomes, formed by ribosomes, mRNAs, several proteins and non-coding RNAs, represent integrated platforms where translational controls take place. However, while the ribosome has been widely studied, the organization of polysomes is still lacking comprehensive understanding. Thus much effort is required in order to elucidate polysome organization and any novel mechanism of translational control that may be embedded. Atomic force microscopy (AFM) is a type of scanning probe microscopy that allows the acquisition of 3D images at nanoscale resolution. Compared to electron microscopy (EM) techniques, one of the main advantages of AFM is that it can acquire thousands of images both in air and in solution, enabling the sample to be maintained under near physiological conditions without any need for staining and fixing procedures. Here, a detailed protocol for the accurate purification of polysomes from mouse brain and their deposition on mica substrates is described. This protocol enables polysome imaging in air and liquid with AFM and their reconstruction as three-dimensional objects. Complementary to cryo-electron microscopy (cryo-EM), the proposed method can be conveniently used for systematically analyzing polysomes and studying their organization.

While ribosome structure has been extensively characterized, the organization of polysomes is still understudied. To overcome this lack of knowledge, we present here a detailed preparation protocol for accurate imaging of mammalian polysomes by atomic force microscopy (AFM) in air and liquid.

يطل في الدماغ Polysomes مع مجهر القوة الذرية

The translational machinery, i.e., the polysome or polyribosome, is one of the biggest and most complex cytoplasmic machineries in cells. Polysomes, ...

Analyzing Synaptic Modulation of Drosophila melanogaster Photoreceptors after Exposure to Prolonged Light

The nervous system has the remarkable ability to adapt and respond to various stimuli. This neural adjustment is largely achieved through plasticity at the synaptic level. The Active Zone (AZ) is the region at the presynaptic membrane that mediates neurotransmitter release and is composed of a dense collection of scaffold proteins. AZs of Drosophila melanogaster (Drosophila) photoreceptors undergo molecular remodeling after prolonged exposure to natural ambient light. Thus the level of neuronal activity can rearrange the molecular composition of the AZ and contribute to the regulation of the functional output.
Starting from the light exposure set-up preparation to the immunohistochemistry, this protocol details how to quantify the number, the spatial distribution, and the delocalization level of synaptic molecules at AZs in Drosophila photoreceptors. Using image analysis software, clusters of the GFP-fused AZ component Bruchpilot were identified for each R8 photoreceptor (R8) axon terminal. Detected Bruchpilot spots were automatically assigned to individual R8 axons. To calculate the distribution of spot frequency along the axon, we implemented a customized software plugin. Each axon&#39;s start-point and end-point were manually defined and the position of each Bruchpilot spot was projected onto the connecting line between start and end-point. Besides the number of Bruchpilot clusters, we also quantified the delocalization level of Bruchpilot-GFP within the clusters. These measurements reflect in detail the spatially resolved synaptic dynamics in a single neuron under different environmental conditions to stimuli.

Analyzing Synaptic Modulation of Drosophila melanogaster Photoreceptors after Exposure to Prolonged Light

Here we show how to quantify the number and spatial distribution of synaptic active zones in Drosophila melanogaster&#160;photoreceptors, highlighted with a genetically encoded molecular marker, and their modulation after prolonged exposure to light.

تحليل متشابك التحوير من<em&gt; ذبابة الفاكهة</em&gt; خلايا مستقبلة للضوء بعد التعرض للضوء لفترات طويلة

The nervous system has the remarkable ability to adapt and respond to various stimuli. This neural adjustment is largely achieved through plasticity at ...

Visualization of the Axonal Projection Pattern of Embryonic Motor Neurons in Drosophila

The establishment of functional neuromuscular circuits relies on precise connections between developing motor axons and target muscles. Motor neurons extend growth cones to navigate along specific pathways by responding to a large number of axon guidance cues that emanate from the surrounding extracellular environment. Growth cone target recognition also plays a critical role in neuromuscular specificity. This work presents a standard immunohistochemistry protocol to visualize motor neuron projections of late stage-16 Drosophila melanogaster embryos. This protocol includes a few key steps, including a genotyping procedure, to sort the desired mutant embryos; an immunostaining procedure, to tag embryos with fasciclin II (FasII) antibody; and a dissection procedure, to generate filleted preparations from fixed embryos. Motor axon projections and muscle patterns in the periphery are much better visualized in flat preparations of filleted embryos than in whole-mount embryos. Therefore, the filleted preparation of fixed embryos stained with FasII antibody provides a powerful tool to characterize the genes required for motor axon pathfinding and target recognition, and it can also be applied to both loss-of-function and gain-of-function genetic screens.

Visualization of the Axonal Projection Pattern of Embryonic Motor Neurons in Drosophila

This work details a standard immunohistochemistry method to visualize motor neuron projections of late stage-16 Drosophila melanogaster embryos. The filleted preparation of fixed embryos stained with FasII antibody provides a powerful tool to characterize the genes required for motor axon pathfinding and target recognition during neural development.

التصور من محور الإسقاط نمط من الخلايا العصبية المحرك الجنينية في<em&gt; ذبابة الفاكهة</em

The establishment of functional neuromuscular circuits relies on precise connections between developing motor axons and target muscles. Motor neurons ...

Isolation of Adult Spinal Cord Nuclei for Massively Parallel Single-nucleus RNA Sequencing

Probing an individual cell&#39;s gene expression enables the identification of cell type and cell state. Single-cell RNA sequencing has emerged as a powerful tool for studying transcriptional profiles of cells, particularly in heterogeneous tissues such as the central nervous system. However, dissociation methods required for single cell sequencing can lead to experimental changes in the gene expression and cell death. Furthermore, these methods are generally restricted to fresh tissue, thus limiting studies on archival and bio-bank material. Single nucleus RNA sequencing (snRNA-Seq) is an appealing alternative for transcriptional studies, given that it accurately identifies cell types, permits the study of tissue that is frozen or difficult to dissociate, and reduces dissociation-induced transcription. Here, we present a high-throughput protocol for rapid isolation of nuclei for downstream snRNA-Seq. This method enables isolation of nuclei from fresh or frozen spinal cord samples and can be combined with two massively parallel droplet encapsulation platforms.

Here, we present a protocol to rapidly isolate high-quality nuclei from the fresh or frozen tissue for downstream massively parallel RNA sequencing. We include detergent-mechanical and hypotonic-mechanical tissue disruption and cell lysis options, both of which can be used for isolation of nuclei.

عزل نويات الحبل الشوكي الكبار لتسلسل الحمض النووي الريبي المفرد-نواة موازية على نطاق واسع

Probing an individual cell&#39;s gene expression enables the identification of cell type and cell state. Single-cell RNA sequencing has emerged as a ...

Chronic Implantation of Whole-cortical Electrocorticographic Array in the Common Marmoset

Electrocorticography (ECoG) allows the monitoring of electrical field potentials from the cerebral cortex with high spatiotemporal resolution. Recent development of thin, flexible ECoG electrodes has enabled conduction of stable recordings of large-scale cortical activity. We have developed a whole-cortical ECoG array for the common marmoset. The array continuously covers almost the entire lateral surface of cortical hemisphere, from the occipital pole to the temporal and frontal poles, and it captures whole-cortical neural activity in one shot. This protocol describes a chronic implantation procedure of the array in the epidural space of the marmoset brain. Marmosets have two advantages regarding ECoG recordings, one being the homologous organization of anatomical structures in humans and macaques, including frontal, parietal, and temporal complexes. The other advantage is that the marmoset brain is lissencephalic and contains a large number of complexes, which are more difficult to access in macaques with ECoG, that are exposed to the brain surface.These features allow direct access to most cortical areas beneath the surface of the brain. This system provides an opportunity to investigate global cortical information processing with high resolutions at a sub-millisecond order in time and millimeter order in space.

We have developed a whole-cortical electrocorticographic array for the common marmoset that continuously covers almost the entire lateral surface of cortex, from the occipital pole to the temporal and frontal poles. This protocol describes a chronic implantation procedure of the array in the epidural space of the marmoset brain.

غرس المزمن من الصفيف اليكتروكورتيكوجرافيك الجامعة القشرية في مرموز المشتركة

Electrocorticography (ECoG) allows the monitoring of electrical field potentials from the cerebral cortex with high spatiotemporal resolution. Recent ...