فإن الكتاب أود أن أشكر ماونا كيا تقنيات للحصول على الدعم التقني. ونشكر أيضا من الكتاب شيلي هسياو للمساعدة التقنية وكاثلين ماك E. لمراجعة نقدية. وأيد هذا العمل من قبل المعاهد الوطنية للصحة في جزء R01CA160986 إلى JCL

1. تحضير المريض <ol style=";text-align:right;direction:rtl"><li style=";text-align:right;direction:rtl"> موافقة المريض لفحص مثانة المقرر التشخيص والإجراءات endourological أخرى مثل استئصال ورم المثانة عبر الاحليل من (TURBT) لCLE. تدرج في الموافقة وصفا للاستخدام فلوريسئين إينترفسكل و / أو عن طريق الوريد وكيلا التباين. استفسار تاريخ رد فعل فرط الحساسية للفلوريسئين. </li><li style=";text-align:right;direction:rtl"> يتم وضع المريض لفحص مثانة (عادة في استخراج الحصاة موقف) وأعدت بطريقة عقيمة. </li><li style=";text-align:right;direction:rtl"> المضي قدما في معيار فحص مثانة الضوء الأبيض (WLC) مع منظار المثانة المرن أو جامدة عن طريق مجرى البول. </li><li style=";text-align:right;direction:rtl"> مسح جميع مناطق المثانة تحت الضوء الأبيض (الشكل 1) ونلاحظ المناطق ذات الأهمية للتحقيق CLE. </li><li style=";text-align:right;direction:rtl"> بالإضافة إلى WLC، قد يتم تصويرها في المثانة مع غيرها من طرائق التصوير الضوئي العيانية بما في ذلك فحص مثانة مضان (الشكل 2A) أو ضيقة النطاق التصوير (الشكل 2B) لمعرفentify مجالات إضافية للاشتباه تليها توصيف مع CLE. </li><li style=";text-align:right;direction:rtl"> استخدام الكي الكهربائي أو استئصال حلقة لوضع علامة بالكي الصغيرة المجاورة للمناطق التي من شأنها أن مصلحة كل من يمكن تصوير وتحتاج فحص لتأكيد المرضية. علامة بالكي يسهل إعادة التوطين للتصوير CLE اللاحقة والخزعة. </li></ol> 2. على النقيض عامل <ol style=";text-align:right;direction:rtl"><li style=";text-align:right;direction:rtl"> إينترفسكل تقطير من عامل تباين <ol style=";text-align:right;direction:rtl"><li style=";text-align:right;direction:rtl"> يعد بحلول تمييع السريرية الصف الصوديوم فلوريسئين 10٪ في المياه المالحة العادية العقيمة (0.9٪ كلوريد الصوديوم) لتركيز المطلوب. على سبيل المثال، وإعداد 400 مل من 0.1٪ من فلوريسئين تمييع 4 مل من فلوريسئين 10٪ في 396 مل من محلول ملحي. </li><li style=";text-align:right;direction:rtl"> إدراج القسطرة البولية المعقمة في المثانة وغرس المخفف عامل تباين بفعل الجاذبية باستخدام القسطرة 60 مل حقنة نصيحه. </li><li style=";text-align:right;direction:rtl"> المشبك القسطرة البولية لعقد المقابل سكنى وكيل لمدة 5 دقائق. </li><li style=";text-align:right;direction:rtl"> استنزاف عامل تباين من المثانة. استخدام 60 مل كاليفورنياtheter معلومات سرية حقنة لتغسل النقيض المتبقية من المثانة باستخدام محلول ملحي، حول 240-300 مل. </li><li style=";text-align:right;direction:rtl"> انتقل إلى التصوير CLE. </li></ol></li><li style=";text-align:right;direction:rtl"> حقنة وريدية من فلوريسئين <ol style=";text-align:right;direction:rtl"><li style=";text-align:right;direction:rtl"> رسم 1.0 مل من فلوريسئين في حقنة. نعلق حقنة لحقن في الوريد وصول 0،5 حتي 1،0 مل من فلوريسئين عن طريق الوريد كما البلعة. تمشيا مع تدفق المياه المالحة العادية. هذا ما يحدث عادة من قبل فريق التخدير في غرفة العمليات. </li><li style=";text-align:right;direction:rtl"> انتقل إلى التصوير CLE. </li></ol></li></ol> 3. CLE التصوير <ol style=";text-align:right;direction:rtl"><li style=";text-align:right;direction:rtl"> إزالة تعقيم CLE التصوير التحقيق (Cellvizio) من عبوته وتوصيله إلى وحدة المسح بالليزر (ماونا كيا تكنولوجيز، باريس، فرنسا) </li><li style=";text-align:right;direction:rtl"> الاحماء ومعايرة مسبار التصوير باتباع إرشادات الشركة المصنعة. </li><li style=";text-align:right;direction:rtl"> إعادة إدراج منظار المثانة أو منظار القطع في المثانة. استخدام عدسة درجة 0 مع منظار المثانة جامدة. </li><li style=";text-align:right;direction:rtl"> دفع التصوير CLE التحقيق على طول التعليم الجامعيقناة rking من منظار المثانة مع تلميح فقط ما وراء منظار المثانة. </li><li style=";text-align:right;direction:rtl"> تحت الضوء الأبيض، حدد موقع المناطق ملحوظ في السابق من الاهتمام. استخدام الري المالحة العادي إضافية حسب الحاجة لرؤية الغشاء المخاطي والحفاظ على انتفاخ معتدل في المثانة. </li><li style=";text-align:right;direction:rtl"> التلاعب CLE التصوير التحقيق مع جهة المشغل لوجه الاتصال المباشر EN من المناطق المثيرة للاهتمام. لباجتزاء البصري للمناطق ذات الاهتمام، وزيادة وانخفاض الضغط بلطف على التحقيق التصوير مع الحفاظ على الاتصال المباشر. <ol style=";text-align:right;direction:rtl"><li style=";text-align:right;direction:rtl"> لتصل إلى الأورام الموجودة في المثانة الأمامي، قد معيار ألباران جسر مساعدة في تسهيل انحراف لجنة التحقيق التصوير للاتصال المباشر مع المنطقة ذات الاهتمام (الشكل 2C). </li></ol></li><li style=";text-align:right;direction:rtl"> المناطق ذات الاهتمام المشترك وتشمل الورم الحليمي، ورم حليمي غير، والانتقال بين العادية التي تظهر الغشاء المخاطي والأورام، وبقع حمامية، والتي يمكن لجميع يمكن تصوير باستخدام إما intraveSICAL أو في الوريد الإدارة فلوريسئين. مطلوب فلوريسئين الوريد للتصوير من السرير بتر ومجرى البول البروستاتا، مجرى البول والقضيب. </li><li style=";text-align:right;direction:rtl"> كما يقوم بمسح التصوير التحقيق المنطقة من الصور سجل الفائدة، لتحليل المستقبل. ملاحظة مورفولوجيا الخلوية الشاملة، والحدود، والتنظيم، وجود الأوعية الدموية الجديدة. </li><li style=";text-align:right;direction:rtl"> عند الانتهاء من التصوير، والتصوير إزالة CLE التحقيق من العمل القناة. </li><li style=";text-align:right;direction:rtl"> إذا رغبت، الحصول على الباردة كأس الخزعات من مناطق ذات أهمية المقابلة التي تم مسحها ضوئيا من قبل CLE. استخدام علامات الكي ومعالم للتسجيل المشاركين للمناطق التي تحتاج فحص المصورة. </li><li style=";text-align:right;direction:rtl"> استكمال TURBT في الروتين. في المرضى الذين تلقوا فلوريسئين الوريد، النظر في استخدام resectoscopes التدفق المستمر لتحسين التصور خلال استئصال، كما هو تفرز عن طريق الكلى فلوريسئين في المثانة حوالي 5 دقائق بعد تناوله عن طريق الحقن. </li><li style=";text-align:right;direction:rtl"> استرجاع التسجيلات الصورة المحفوظة من معالج مبائر وconduط م تحليل الصور. </li></ol>

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الإعلان عن أي تضارب في المصالح.

تحقيق والحفاظ على الاتصال الصلبة وجه EN بين التحقيق والتصوير الغشاء المخاطي المثانة هي الخطوة الأكثر أهمية في الحصول على أفضل جودة الصورة. هناك ما يقرب من 3-5 منحنى التعلم المريض لتطوير مهارة في التلاعب التحقيق التصوير واجراء التحقيق المطرد خلال الحصول على الصور....

<table border="1" style=";text-align:right;direction:rtl"><tbody><tr><td> اسم كاشف </td><td> شركة </td><td> كتالوج رقم </td><td> التعليقات (اختياري) </td></tr><tr><td> Cellvizio المجموعة 100 </td><td> ماونا كيا تكنولوجيا </td><td> المجموعة 100 </td><td> يتضمن معالج مبائر وLSU: F400-V2 في 488 نانومتر </td></tr><tr><td> Cellvizio متحد البؤر Miniprobe </td><td> ماونا كيا تكنولوجيا </td><td> Gastroflex UHD </td><td></td></tr><tr><td> AK-FLUOR 10٪ </td><td> Akorn، وشركة </td><td> NDC 17478-253-10 </td><td></td></tr></tbody></table>

probe-based confocal laser endomicroscopy of the urinary tract: the technique

التحقيق القائم على الليزر مبائر endomicroscopy (CLE) هو الناشئة تكنولوجيا التصوير الضوئي التي تمكن في الوقت الحقيقي في الجسم الحي المجهري من خلال التنظير الأسطح المخاطية القياسية. مع التطبيقات حاليا في الجهاز التنفسي 1 و الجهاز الهضمي، كما تم استكشاف 2-6 CLE في المسالك البولية لتشخيص سرطان المثانة. مورفولوجيا الخلوية 7-10 و المعمارية المصغرة الأنسجة لا يمكن حلها لقرار مقياس ميكرون في الوقت الحقيقي، بالإضافة إلى دينامية تصوير الأوعية الدموية الطبيعية والمرضية .. 7 التحقيق المستندة إلى نظام CLE (Cellvizio، ماونا كيا تكنولوجيز، فرنسا) ويتكون من مسبار التصوير الالياف البصرية القابلة لإعادة الاستخدام بالإضافة إلى وحدة المسح بالليزر نانومتر 488. يتم إدراج التحقيق التصوير في القنوات العاملة على مستوى المناظير المرنة والصلبة. المنظار القائم على نظام CLE (Optiscan، استراليا)، الذي الوظيفيه endomicroscopy مبائرتم دمج Y على المنظار، كما يستخدم في القناة الهضمية. نظرا لقطرها أكبر نطاق، ومع ذلك، يقتصر حاليا تطبيق في المسالك البولية لاستخدام الجسم الحي السابقين. يتم الحصول على الصور 11 متحد البؤر من خلال الاتصال المباشر من التحقيق مع التصوير النسيج المستهدف وكما هو مسجل الفيديو متواليات. كما في القناة الهضمية، endomicroscopy في المسالك البولية يتطلب exogenenous النقيض عوامل الأكثر شيوعا فلوريسئين، والتي يمكن أن تدار عن طريق الوريد أو intravesically. إدارة إينترفسكل هو وسيلة راسخة لتقديم وكلاء الدوائية محليا مع الحد الأدنى من سمية جهازية التي هي فريدة من نوعها في المسالك البولية. البقع بسرعة فلوريسئين المصفوفة خارج الخلية ويحتوي على الملف الشخصي السلامة المعمول. تحقيقات التصوير بأقطار مختلفة 12 من تمكين التوافق مع المناظير عيارات مختلفة. حتى الآن، تم تقييم 1.4 و 2.6 ملم مع تحقيقات مرنة وريتنظير المثانة دائرة المخابرات العامة 10 توافر الأخيرة من &lt;1 مسبار التصوير 13 مم يفتح إمكانية CLE في المسالك البولية العلوي خلال ureteroscopy. مضان تنظير المثانة (التشخيص الضوئي أي) وضيقة النطاق التصوير تضاف المنظار القائم على طرائق التصوير الضوئي 14 التي يمكن دمجها مع CLE لتحقيق التصوير المتعدد الوسائط في المسالك البولية. في المستقبل، قد يكون جانب CLE مع عوامل التباين الجزيئي مثل الببتيدات fluorescently المسمى 15 و الأجسام المضادة للتصوير بالمنظار من العمليات المرض مع خصوصية الجزيئية.

يتم حفظ الصور CLE والرمادي ملفات الفيديو تسلسل في 12 لقطة في الثانية. ويتم تفسير الصور في الوقت الحقيقي، ويمكن استخدامها لصنع القرار السريري تؤثر بموجب بروتوكول الفحص. تحليل حاليا، والذي يتضمن مراجعة للتسلسل الفيديو، ومعالجة الصور إضافية مثل mosaicing، 7 والمقارنة م?...

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Probe-based Confocal Laser Endomicroscopy of the Urinary Tract: The Technique

التحقيق القائم على الليزر endomicroscopy مبائر تمكن في الوقت الحقيقي المجهري في المسالك البولية أثناء تنظير المثانة الإنسان، وتوفير دينامية، والتصوير intravital من الحالات المرضية مثل سرطان المثانة لقرار الخلوية. قد Endomicroscopy زيادة دقة التشخيص على مستوى التنظير الضوء الأبيض أثناء العملية وتوفير التوجيه لتحسين صورة استئصال الجراحي.

التحقيق المستندة إلى Endomicroscopy الليزر متحد البؤر في المسالك البولية: تقنية

Probe-based  confocal laser endomicroscopy (CLE) is an emerging optical imaging technology  that enables real-time in vivo microscopy of mucosal surfaces ...

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20.7K Views.  Stanford University School of Medicine. التحقيق القائم على الليزر endomicroscopy مبائر تمكن في الوقت الحقيقي المجهري في المسالك البولية أثناء تنظير المثانة الإنسان، وتوفير دينامية، والتصوير intravital من الحالات المرضية مثل سرطان المثانة لقرار الخلوية. قد Endomicroscopy زيادة دقة التشخيص على مستوى التنظير الضوء الأب...

Video: Probe-based Confocal Laser Endomicroscopy of the Urinary Tract: The Technique

Oral Biofilm Analysis of Palatal Expanders by Fluorescence In-Situ Hybridization and Confocal Laser Scanning Microscopy

Confocal laser scanning microscopy (CLSM) of natural heterogeneous biofilm is today facilitated by a comprehensive range of staining techniques, one of them being fluorescence in situ hybridization (FISH).1,2 We performed a pilot study in which oral biofilm samples collected from fixed orthodontic appliances (palatal expanders) were stained by FISH, the objective being to assess the three-dimensional organization of natural biofilm and plaque accumulation.3,4 FISH creates an opportunity to stain cells in their native biofilm environment by the use of fluorescently labeled 16S rRNA-targeting probes.4-7,19 Compared to alternative techniques like immunofluorescent labeling, this is an inexpensive, precise and straightforward labeling technique to investigate different bacterial groups in mixed biofilm consortia.18,20 General probes were used that bind to Eubacteria (EUB338 + EUB338II + EUB338III; hereafter EUBmix),8-10 Firmicutes (LGC354 A-C; hereafter LGCmix),9,10 and Bacteroidetes (Bac303).11 In addition, specific probes binding to Streptococcus mutans (MUT590)12,13 and Porphyromonas gingivalis (POGI)13,14 were used. The extreme hardness of the surface materials involved (stainless steel and acrylic resin) compelled us to find new ways of preparing the biofilm. As these surface materials could not be readily cut with a cryotome, various sampling methods were explored to obtain intact oral biofilm. The most workable of these approaches is presented in this communication. Small flakes of the biofilm-carrying acrylic resin were scraped off with a sterile scalpel, taking care not to damage the biofilm structure. Forceps were used to collect biofilm from the steel surfaces. Once collected, the samples were fixed and placed directly on polysine coated glass slides. FISH was performed directly on these slides with the probes mentioned above. Various FISH protocols were combined and modified to create a new protocol that was easy to handle.5,10,14,15 Subsequently the samples were analyzed by confocal laser scanning microscopy. Well-known configurations3,4,16,17 could be visualized, including mushroom-style formations and clusters of coccoid bacteria pervaded by channels. In addition, the bacterial composition of these typical biofilm structures were analyzed and 2D and 3D images created.

We present a protocol for structural and compositional analysis of natural oral biofilm from orthodontic appliances with in situ hybridization (FISH) and confocal laser scanning microscopy (CLSM). Oral biofilm samples were collected from palatal expanders, scraping acrylic-resin flakes off their surface and referring them for molecular processing.

عن طريق الفم من تحليل بيوفيلم المتوسعون حلقي بواسطة التهجين في الموضع الإسفار ومتحد البؤر المسح الضوئي ليزر مجهرية

Confocal laser scanning microscopy (CLSM) of natural heterogeneous biofilm is today facilitated by a comprehensive range of staining techniques, one of ...

Bladder Smooth Muscle Strip Contractility as a Method to Evaluate Lower Urinary Tract Pharmacology

We describe an in vitro method to measure bladder smooth muscle contractility, and its use for investigating physiological and pharmacological properties of the smooth muscle as well as changes induced by pathology. This method provides critical information for understanding bladder function while overcoming major methodological difficulties encountered in in vivo experiments, such as surgical and pharmacological manipulations that affect stability and survival of the preparations, the use of human tissue, and/or the use of expensive chemicals. It also provides a way to investigate the properties of each bladder component (i.e. smooth muscle, mucosa, nerves) in healthy and pathological conditions.
The urinary bladder is removed from an anesthetized animal, placed in Krebs solution and cut into strips. Strips are placed into a chamber filled with warm Krebs solution. One end is attached to an isometric tension transducer to measure contraction force, the other end is attached to a fixed rod. Tissue is stimulated by directly adding compounds to the bath or by electric field stimulation electrodes that activate nerves, similar to triggering bladder contractions in vivo. We demonstrate the use of this method to evaluate spontaneous smooth muscle contractility during development and after an experimental spinal cord injury, the nature of neurotransmission (transmitters and receptors involved), factors involved in modulation of smooth muscle activity, the role of individual bladder components, and species and organ differences in response to pharmacological agents. Additionally, it could be used for investigating intracellular pathways involved in contraction and/or relaxation of the smooth muscle, drug structure-activity relationships and evaluation of transmitter release.
The in vitro smooth muscle contractility method has been used extensively for over 50 years, and has provided data that significantly contributed to our understanding of bladder function as well as to pharmaceutical development of compounds currently used clinically for bladder management.

This manuscript presents a simple, yet powerful, in vitro method for evaluating smooth muscle contractility in response to pharmacological agents or nerve stimulation. Main applications are drug screening and understanding tissue physiology, pharmacology,&#160;and pathology.

المثانة العضلات الملساء قطاع انقباض كوسيلة لتقييم المسالك البولية السفلى الصيدلة

We describe an in vitro method to measure bladder smooth muscle contractility, and its use for investigating physiological and pharmacological properties ...

Rose Bengal Photothrombosis by Confocal Optical Imaging In Vivo: A Model of Single Vessel Stroke

In vivo imaging techniques have increased in utilization due to recent advances in imaging dyes and optical technologies, allowing for the ability to image cellular events in an intact animal. Additionally, the ability to induce physiological disease states such as stroke in vivo increases its utility. The technique described herein allows for physiological assessment of cellular responses within the CNS following a stroke and can be adapted for other pathological conditions being studied. The technique presented uses laser excitation of the photosensitive dye Rose Bengal in vivo to induce a focal ischemic event in a single blood vessel. 
The video protocol demonstrates the preparation of a thin-skulled cranial window over the somatosensory cortex in a mouse for the induction of a Rose Bengal photothrombotic event keeping injury to the underlying dura matter and brain at a minimum. Surgical preparation is initially performed under a dissecting microscope with a custom-made surgical/imaging platform, which is then transferred to a confocal microscope equipped with an inverted objective adaptor. Representative images acquired utilizing this protocol are presented as well as time-lapse sequences of stroke induction. This technique is powerful in that the same area can be imaged repeatedly on subsequent days facilitating longitudinal in vivo studies of pathological processes following stroke.

Rose Bengal Photothrombosis by Confocal Optical Imaging In Vivo:  A Model of Single Vessel Stroke

Here, we describe a semi-invasive optical microscopy approach for the induction of a Rose Bengal photothrombotic clot in the somatosensory cortex of a mouse in vivo. The technical aspects of the imaging procedure are described from induction of a photothrombotic event to application and data collection.

روز البنغال Photothrombosis بواسطة التصوير الضوئي متحد البؤر<em&gt; في فيفو</em&gt;: نموذج لسفينة واحدة السكتة الدماغية

In vivo imaging techniques have increased in utilization due to recent advances in imaging dyes and optical technologies, allowing for the ability to ...

Using a Laminating Technique to Perform Confocal Microscopy of the Human Sclera

The sclera is a dense connective tissue that covers and protects the eye. It mainly consists of dense collagen bundles (types I, III, IV, V, VI, and VII). Due to its autofluorescence, opaqueness, and thickness, it has not been found suitable for confocal microscopy. An alternative approach to the one presented here, which uses formalin-fixed sclera embedded in paraffin for immunohistochemistry, has technical challenges, especially when preheating the tissue for antigen retrieval. Since the sclera is relatively poor in both cells and vessels, the use of larger tissue samples was explored to help prevent overlooking cells and to understand their localization in relation to vessels and other anatomical sites. To allow for the analysis of larger tissue samples under the confocal microscope, a laminating technique was performed to create thin layers from the sclera. Following the analysis of results of CD31 blood vessels and lymphatic vessel endothelial hyaluronan receptor 1 (LYVE1) positive cells, for which approval for scientific examination was obtained, the advantages and limitations of this method are discussed.

Human sclera tissue is mainly collagen; therefore, it is not easily usable for immunohistochemistry. To achieve the goal of performing immunohistochemistry for confocal microscopy of scleral tissue, a laminating technique was used.

باستخدام تقنية الترقق لتنفيذ متحد البؤر المجهري للالصلبة الإنسان


The sclera is a dense connective tissue that covers and protects the eye. It mainly consists of dense collagen bundles (types I, III, IV, V, VI, and ...

Imaging Metals in Brain Tissue by Laser Ablation - Inductively Coupled Plasma - Mass Spectrometry (LA-ICP-MS)

Metals are found ubiquitously throughout an organism, with their biological role dictated by both their chemical reactivity and abundance within a specific anatomical region. Within the brain, metals have a highly compartmentalized distribution, depending on the primary function they play within the central nervous system. Imaging the spatial distribution of metals has provided unique insight into the biochemical architecture of the brain, allowing direct correlation between neuroanatomical regions and their known function with regard to metal-dependent processes. In addition, several age-related neurological disorders feature disrupted metal homeostasis, which is often confined to small regions of the brain that are otherwise difficult to analyze. Here, we describe a comprehensive method for quantitatively imaging metals in the mouse brain, using laser ablation - inductively coupled plasma - mass spectrometry (LA-ICP-MS) and specially designed image processing software. Focusing on iron, copper and zinc, which are three of the most abundant and disease-relevant metals within the brain, we describe the essential steps in sample preparation, analysis, quantitative measurements and image processing to produce maps of metal distribution within the low micrometer resolution range. This technique, applicable to any cut tissue section, is capable of demonstrating the highly variable distribution of metals within an organ or system, and can be used to identify changes in metal homeostasis and absolute levels within fine anatomical structures.

Imaging Metals in Brain Tissue by Laser Ablation - Inductively Coupled Plasma - Mass Spectrometry LA-ICP-MS

Quantitatively mapping metals in tissue by laser ablation - inductively coupled plasma - mass spectrometry (LA-ICP-MS) is a sensitive analytical technique that can provide new insight into how metals participate in normal function and disease processes. Here, we describe a protocol for quantitatively imaging metals in thin sections of mouse neurological tissue.

المعادن التصوير في أنسجة المخ عن طريق الاستئصال بالليزر - إلى جانب بالحث البلازما - الطيف الكتلي (LA-ICP-MS)

Metals are found ubiquitously throughout an organism, with their biological role dictated by both their chemical reactivity and abundance within a ...

Spatial Quantification of Drugs in Pulmonary Tuberculosis Lesions by Laser Capture Microdissection Liquid Chromatography Mass Spectrometry (LCM-LC/MS)

Tuberculosis is still a leading cause of morbidity and mortality worldwide. Improvements to existing drug regimens and the development of novel therapeutics are urgently required. The ability of dosed TB drugs to reach and sterilize bacteria within poorly-vascularized necrotic regions (caseum) of pulmonary granulomas is crucial for successful therapeutic intervention. Effective therapeutic regimens must therefore contain drugs with favorable caseum penetration properties. Current LC/MS methods for quantifying drug levels in biological tissues have limited spatial resolution capabilities, making it difficult to accurately determine absolute drug concentrations within small tissue compartments such as those found within necrotic granulomas. Here we present a protocol combining laser capture microdissection (LCM) of pathologically-distinct tissue regions with LC/MS quantification. This technique provides absolute quantification of drugs within granuloma caseum, surrounding cellular lesion and uninvolved lung tissue and, therefore, accurately determines whether bactericidal concentrations are being achieved. In addition to tuberculosis research, the technique has many potential applications for spatially-resolved quantification of drugs in diseased tissues.

Spatial Quantification of Drugs in Pulmonary Tuberculosis Lesions by Laser Capture Microdissection Liquid Chromatography Mass Spectrometry LCM-LC/MS

Here, we describe a protocol using laser capture microdissection coupled with LC/MS analysis to spatially-quantify drug distributions within pulmonary tuberculosis granulomas. The approach has broad applicability to quantifying drug concentrations within tissues at high spatial detail.

التقاط الكمي المكانية للمخدرات في الآفات السل الرئوي بالليزر اللوني السائل ميكروديسيكشن الكتلي (LCM-LC/MS)

Tuberculosis is still a leading cause of morbidity and mortality worldwide. Improvements to existing drug regimens and the development of novel ...

Ultrasonography of the Adult Male Urinary Tract for Urinary Functional Testing

The incidence of clinical benign prostatic hyperplasia (BPH) and lower urinary tract symptoms (LUTS) is increasing due to the aging population, resulting in a significant economic and quality of life burden. Transgenic and other mouse models have been developed to recreate various aspects of this multifactorial disease; however, methods to accurately quantitate urinary dysfunction and the effectiveness of new therapeutic options are lacking. Here, we describe a method that can be used to measure bladder volume and detrusor wall thickness, urinary velocity, void volume and void duration, and urethral diameter. This would allow for the evaluation of disease progression and treatment efficacy over time. Mice were anesthetized with isoflurane, and the bladder was visualized by ultrasound. For non-contrast imaging, a 3D image was taken of the bladder to calculate volume and evaluate shape; the bladder wall thickness was measured from this image. For contrast-enhanced imaging, a catheter was placed through the dome of the bladder using a 27-gauge needle connected to a syringe by PE50 tubing. A bolus of 0.5 mL of contrast was infused into the bladder until a urination event occurred. Urethral diameter was determined at the point of the Doppler velocity sample window during the first voiding event. Velocity was measured for each subsequent event yielding a flow rate. In conclusion, high frequency ultrasound proved to be an effective method for assessing bladder and urethral measurements during urinary function in mice. This technique may be useful in the assessment of novel therapies for BPH/LUTS in an experimental setting.

We describe the use of high frequency ultrasound with contrast imaging as a method to measure bladder volume, bladder wall thickness, urine velocity, void volume, void duration, and urethral diameter. This strategy can be used to assess voiding dysfunction and treatment efficacy in various mouse models of lower urinary tract dysfunction (LUTD).

التصوير بالموجات فوق الصوتية للمسالك البولية الذكور الكبار للاختبار الوظيفي البولي

The incidence of clinical benign prostatic hyperplasia (BPH) and lower urinary tract symptoms (LUTS) is increasing due to the aging population, resulting ...

Technical Modification of the Terminal Ureter During Total Transperitoneal Laparoscopic Nephroureterectomy for Upper Urinary Tract Urothelial Carcinoma

Upper tract urothelial carcinoma (UTUC) accounts for 5%&ndash;10% of all urothelial tumors. Radical nephroureterectomy is the standard treatment procedure. At present, different choices still exist for treating the ureteral end during laparoscopic ureteral bladder sleeve resection. Our center has adopted a new method for treating the ureteral end. This new method can increase the operating space and reduce the difficulty of the surgery compared with current methods.

Here, we present a protocol to increase the surgical field of view and reduce the difficulty of total transperitoneal laparoscopic nephroureterectomy surgery by precutting the umbilical ligament before treating the terminal ureter.

التعديل التقني لحالب المحطة الطرفية خلال استئصال الكلية عبر الصفاق بالمنظار لسرطان المسالك البولية العلوية

Upper tract urothelial carcinoma (UTUC) accounts for 5%&ndash;10% of all urothelial tumors. Radical nephroureterectomy is the standard treatment ...

Laser Doppler Perfusion Imaging in the Mouse Hindlimb

Blood flow recovery is a critical outcome measure after experimental hindlimb ischemia or ischemia-reperfusion. Laser Doppler perfusion imaging (LDPI) is a common, noninvasive, repeatable method for assessing blood flow recovery. The technique calculates overall blood flow in the sampled tissue from the Doppler shift in frequency caused when a laser hits moving red blood cells. Measurements are expressed in arbitrary perfusion units, so the contralateral non-intervened upon leg is usually used to help control measurements. Measurement depth is in the range of 0.3-1 mm; for hindlimb ischemia, this means that dermal perfusion is assessed. Dermal perfusion is dependent on several factors&#8212;most importantly skin temperature and anesthetic agent, which must be carefully controlled to result in reliable readings. Furthermore, hair and skin pigmentation can alter the ability of the laser to either reach or penetrate to the dermis. This article demonstrates the technique of LDPI in the mouse hindlimb.

Here, we present a protocol that demonstrates the technique and necessary controls for Laser Doppler perfusion imaging to measure blood flow in the mouse hindlimb.

الليزر دوبلر Perfusion التصوير في هندليمب الماوس

Blood flow recovery is a critical outcome measure after experimental hindlimb ischemia or ischemia-reperfusion. Laser Doppler perfusion imaging (LDPI) is ...

Characterization of a Novel Human Organotypic Retinal Culture Technique

Previous human organotypic retinal culture (HORC) models have utilized detached retinas; however, without the structural support conferred by retinal pigment epithelium-choroid (RPE-choroid) and sclera, the integrity of the fragile retina can easily be compromised. The aim of this study was to develop a novel HORC model that contains the retina, RPE-choroid and sclera to maintain retinal integrity when culturing retinal explants.
After cutting circumferentially along the limbus to remove iris and lens, four deep incisions were made to flatten the eyecup. In contrast to previous HORC protocols, a trephine was used to cut through not only the retina but also the RPE-choroid and sclera. The resultant triple-layered explants were cultured for 72 h. Hematoxylin and Eosin staining (H&#38;E) was used to assess anatomical structures and retinal explants were further characterized by immunohistochemistry (IHC) for apoptosis, M&#252;ller cell integrity and retinal inflammation. To confirm the possibility of disease induction, explants were exposed to high glucose (HG) and pro-inflammatory cytokines (Cyt), to mimic diabetic retinopathy (DR). The Luminex magnetic bead assay was used to measure DR-related cytokines released into the culture medium.
H&#38;E staining revealed distinct retinal lamellae and compact nuclei in retinal explants with the underlying RPE-choroid and sclera, while retinas without the underlying structures exhibited reduced thickness and severe nuclei loss. IHC results indicated absence of apoptosis and retinal inflammation as well as preserved M&#252;ller cell integrity. The Luminex&#160;assays showed significantly increased secretion of DR-associated pro-inflammatory cytokines in retinal explants exposed to HG + Cyt relative to baseline levels at 24 h.
We successfully developed and characterized a novel HORC protocol in which retinal integrity was preserved without apoptosis or retinal inflammation. Moreover, the induced secretion of DR-associated pro-inflammatory biomarkers when exposing retinal explants to HG + Cyt suggests that this model could be used for clinically translatable retinal disease studies.

This study aims to develop a novel human organotypic retinal culture (HORC) model that prevents compromising retinal integrity during explant handling. This is achieved by culturing the retina with the overlying vitreous and the underlying retinal pigment&#160;epithelium-choroid (RPE-choroid) and sclera.

توصيف رواية الإنسان Organotypic تقنية ثقافة الشبكية

Previous human organotypic retinal culture (HORC) models have utilized detached retinas; however, without the structural support conferred by retinal ...