ablation-neuronal-population-using-two-photon-laser-its-assessment

Ablation of a Neuronal Population Using a Two-photon Laser and Its Assessment Using Calcium Imaging and Behavioral Recording in Zebrafish Larvae

To identify the role of a subpopulation of neurons in behavior, it is essential to test the consequences of blocking its activity in living animals. Laser ...

SOKENDAI (The Graduate University for Advanced Studies)

Visualization of neuronal activity during animal behavior is a critical step in understanding how the brain generates behavior. In the model vertebrate zebrafish, imaging of the brain has been done mostly by using immobilized fish. Here, we describe a novel method to image neuronal activity of the larval zebrafish brain during prey capture behavior. We expressed a genetically encoded fluorescent calcium indicator, GCaMP, in the optic tectum of the midbrain using the Gal4-UAS system. Tectal activity was then imaged in unrestrained larvae during prey perception. Since larval zebrafish swim only intermittently, detection of the neuronal activity is possible between swimming bouts. Our method makes functional brain imaging under natural behavioral conditions feasible and will greatly benefit the study of neuronal activities that evoke animal behaviors.

Akira Muto

Division of Molecular and Developmental Biology,
National Institute of Genetics,
Department of Genetics,
Division of Molecular and Developmental Biology, National Institute of Genetics, Department of Genetics

SOKENDAI (The Graduate University for Advanced Studies)

Ablation of a Neuronal Population Using a Two-photon Laser and Its Assessment Using Calcium Imaging and Behavioral Recording in Zebrafish Larvae

Akira Muto

Division of Molecular and Developmental Biology,National Institute of Genetics,Department of Genetics,Division of Molecular and Developmental Biology, National Institute of Genetics, Department of Genetics

SOKENDAI (The Graduate University for Advanced Studies)

Ablation of a Neuronal Population Using a Two-photon Laser and Its Assessment Using Calcium Imaging and Behavioral Recording in Zebrafish Larvae

Division of Molecular and Developmental Biology,
National Institute of Genetics,
Department of Genetics,
Division of Molecular and Developmental Biology, National Institute of Genetics, Department of Genetics