Maarten Merkx

Cover Picture.

Dioxygen Activation and Methane Hydroxylation by Soluble Methane Monooxygenase: A Tale of Two Irons and Three Proteins A list of abbreviations can be found in Section 7.

Why OrfY? Characterization of MMOD, a long overlooked component of the soluble methane monooxygenase from Methylococcus capsulatus (Bath).

Determination by X-ray absorption spectroscopy of the Fe-Fe separation in the oxidized form of the hydroxylase of methane monooxygenase alone and in the presence of MMOD.

Copper-dependent protein-protein interactions studied by yeast two-hybrid analysis.

Preparation and X-ray structures of metal-free, dicobalt and dimanganese forms of soluble methane monooxygenase hydroxylase from Methylococcus capsulatus (Bath).

Successful recombinant production of Allochromatium vinosum cytochrome c' requires coexpression of cmm genes in heme-rich Escherichia coli JCB712.

Electrochemical reduction of NO by hemin adsorbed at pyrolitic graphite.

Multivalent peptide and protein dendrimers using native chemical ligation.

Heme release in myoglobin-DDAB films and its role in electrochemical NO reduction.

Fluorescently labeled collagen binding proteins allow specific visualization of collagen in tissues and live cell culture.

Ratiometric fluorescent sensor proteins with subnanomolar affinity for Zn(II) based on copper chaperone domains.

Quantitative understanding of the energy transfer between fluorescent proteins connected via flexible peptide linkers.

Electron transfer and ligand binding to cytochrome c' immobilized on self-assembled monolayers.

Allocation of substance use disorder patients to appropriate levels of care: feasibility of matching guidelines in routine practice in Dutch treatment centres.

Protein-liposome conjugates using cysteine-lipids and native chemical ligation.

Reorganization of immobilized horse and yeast cytochrome c induced by pH changes or nitric oxide binding.

Variation of linker length in ratiometric fluorescent sensor proteins allows rational tuning of Zn(II) affinity in the picomolar to femtomolar range.

Evidence for heme release in layer-by-layer assemblies of myoglobin and polystyrenesulfonate on pyrolitic graphite.

Enhanced sensitivity of FRET-based protease sensors by redesign of the GFP dimerization interface.

Ligand-induced monomerization of Allochromatium vinosum cytochrome c' studied using native mass spectrometry and fluorescence resonance energy transfer.

High resolution imaging of collagen organisation and synthesis using a versatile collagen specific probe.

Human copper transporter 2 is localized in late endosomes and lysosomes and facilitates cellular copper uptake.

Imaging collagen in intact viable healthy and atherosclerotic arteries using fluorescently labeled CNA35 and two-photon laser scanning microscopy.

Branched KLVFF tetramers strongly potentiate inhibition of beta-amyloid aggregation.

Site-specific protein and peptide immobilization on a biosensor surface by pulsed native chemical ligation.

Ratiometric detection of Zn(II) using chelating fluorescent protein chimeras.

Mapping preferred sites for fluorescent labeling by combining fluorescence and MS analysis of tryptic CNA35 protein digests.

His-tags as Zn(II) binding motifs in a protein-based fluorescent sensor.

Biomechanical properties of abdominal aortic aneurysms assessed by simultaneously measured pressure and volume changes in humans.

One-step refolding and purification of disulfide-containing proteins with a C-terminal MESNA thioester.

Two-photon microscopy on vital carotid arteries: imaging the relationship between collagen and inflammatory cells in atherosclerotic plaques.

Multivalent choline dendrimers as potent inhibitors of pneumococcal cell-wall hydrolysis.

Efficient and chemoselective surface immobilization of proteins by using aniline-catalyzed oxime chemistry.

Collagen targeting using protein-functionalized micelles: the strength of multiple weak interactions.

Feasibility and validity of low-budget telephonic follow-up interviews in routine outcome monitoring of substance abuse treatment.

Noncovalent synthesis of protein dendrimers.

Efficient, chemoselective synthesis of immunomicelles using single-domain antibodies with a C-terminal thioester.

High-affinity peptide-based collagen targeting using synthetic phage mimics: from phage display to dendrimer display.

Genetically encoded FRET sensors to monitor intracellular Zn2+ homeostasis.

Exchange kinetics of protein-functionalized micelles and liposomes studied by Förster resonance energy transfer.

Protease-activatable collagen targeting based on protein cyclization.

Collagen targeting using multivalent protein-functionalized dendrimers.

Antibody detection by using a FRET-based protein conformational switch.

The binding of CNA35 contrast agents to collagen fibrils.

Guidelines for allocating outpatient alcohol abusers to levels of care: Predictive validity.

Dendrimer display of tumor-homing peptides.

Engineering protein switches: sensors, regulators, and spare parts for biology and biotechnology.

From phage display to dendrimer display: insights into multivalent binding.

Choline dendrimers as generic scaffolds for the non-covalent synthesis of multivalent protein assemblies.

NMR characterization of a Cu(I)-bound peptide model of copper metallochaperones: insights on the role of methionine.

Chemoselective protein and peptide immobilization on biosensor surfaces.

Semi-synthesis of a protease-activatable collagen targeting probe.

Reengineering of a fluorescent zinc sensor protein yields the first genetically encoded cadmium probe.

In vivo characterization of a new abdominal aortic aneurysm mouse model with conventional and molecular magnetic resonance imaging.

Colorful calcium sensors.

Calculation of transition dipole moment in fluorescent proteins--towards efficient energy transfer.

Accuracy and precision of vessel area assessment: manual versus automatic lumen delineation based on full-width at half-maximum.

Monitoring bile acid transport in single living cells using a genetically encoded Förster resonance energy transfer sensor.

Tuning the metal binding site specificity of a fluorescent sensor protein: from copper to zinc and back.

Predictive validity of treatment allocation guidelines on drinking outcome in alcohol-dependent patients.

Supramolecular control of enzyme activity through cucurbit[8]uril-mediated dimerization.

The benefit of non contrast-enhanced magnetic resonance angiography for predicting vascular access surgery outcome: a computer model perspective.

Non contrast-enhanced MRA versus ultrasound blood vessel assessment to determine the choice of hemodialysis vascular access.

Switchable reporter enzymes based on mutually exclusive domain interactions allow antibody detection directly in solution.

Robust red FRET sensors using self-associating fluorescent domains.

Rational design of FRET sensor proteins based on mutually exclusive domain interactions.

No washing, less waiting: engineering biomolecular reporters for single-step antibody detection in solution.

MagFRET: the first genetically encoded fluorescent Mg2+ sensor.

Dynamic imaging of cytosolic zinc in Arabidopsis roots combining FRET sensors and RootChip technology.

Engineering genetically encoded FRET sensors.

Mitochondrial and ER-targeted eCALWY probes reveal high levels of free Zn2+.

Genetically-encoded FRET-based sensors for monitoring Zn(2+) in living cells.

DNA-directed control of enzyme-inhibitor complex formation: a modular approach to reversibly switch enzyme activity.

Quantifying stickiness: thermodynamic characterization of intramolecular domain interactions to guide the design of förster resonance energy transfer sensors.

Treatment outcome of alcohol use disorder outpatients with or without medically assisted detoxification.

Colorful protein-based fluorescent probes for collagen imaging.

Antibody activation using DNA-based logic gates.

Efficient Synthesis of Peptide and Protein Functionalized Pyrrole-Imidazole Polyamides Using Native Chemical Ligation.

eZinCh-2: A Versatile, Genetically Encoded FRET Sensor for Cytosolic and Intraorganelle Zn(2+) Imaging.

Inhibition of human copper trafficking by a small molecule significantly attenuates cancer cell proliferation.