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Real Time Monitoring of Intracellular Bile Acid Dynamics Using a Genetically Encoded FRET-based Bile Acid Sensor

9.8K Views

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09:21 min

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January 4th, 2016

DOI :

10.3791/53659-v

January 4th, 2016

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9,762 Views

Sandra Van de Wiel¹, Maarten Merkx²^,³, Stan Van de Graaf¹

¹Tytgat Institute for Liver and Intestinal Research, Department of Gastroenterology and Hepatology, Academic Medical Center, ²Laboratory of Chemical Biology, Institute of Complex Molecular Systems (ICMS), ³Department of Biomedical Engineering, Eindhoven University of Technology

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Summary

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Genetically Encoded FRET based Bile Acid Sensor

Bile Acid Transport

Bile Acid Dynamics

Glucose Metabolism

Lipid Metabolism

Energy Metabolism

FXR Nuclear Bile Acid Receptor

Cerulean Fluorophore

Citrine Fluorophore

Nuclear Localization Signal

Cytoplasmic Localization

Live Cell Imaging

Leibovitz L 15 Culture Medium

DMEM With Phenol Red

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