Rainer Fischer

A carcinoembryonic antigen-specific diabody produced in tobacco.

Uptake of a fluorescent marker in plant cells is sensitive to brefeldin A and wortmannin.

Intracellular concentration measurements in adherent cells: a comparison of import efficiencies of cell-permeable peptides.

Plantibodies: applications, advantages and bottlenecks.

Construction and characterization of a single-chain antibody fragment derived from thymus of a patient with myasthenia gravis.

Targeting tryptophan decarboxylase to selected subcellular compartments of tobacco plants affects enzyme stability and in vivo function and leads to a lesion-mimic phenotype.

A quantitative validation of fluorophore-labelled cell-permeable peptide conjugates: fluorophore and cargo dependence of import.

Production of tumor-specific antibodies in tobacco.

Simultaneous occurrence of Hodgkin's lymphoma and eosinophilic granuloma: a potential pitfall in positron emission tomography imaging.

Production of antibodies in plants and their use for global health.

'Molecular farming' of antibodies in plants.

The glutathione synthetase of Schizosaccharomyces pombe is synthesized as a homodimer but retains full activity when present as a heterotetramer.

Extending the applicability of carboxyfluorescein in solid-phase synthesis.

An unusual internal ribosomal entry site of inverted symmetry directs expression of a potato leafroll polerovirus replication-associated protein.

A young patient with end-stage renal disease, dyspnoea, weakness, peripheral neuropathy and an unsuspected underlying disease.

The recombinant anti-EGF receptor immunotoxin 425(scFv)-ETA' suppresses growth of a highly metastatic pancreatic carcinoma cell line.

Production of therapeutic antibodies in plants.

Molecular farming in plants: host systems and expression technology.

Recombinant CD64-specific single chain immunotoxin exhibits specific cytotoxicity against acute myeloid leukemia cells.

A stepwise dissection of the intracellular fate of cationic cell-penetrating peptides.

Structure property analysis of pentamethine indocyanine dyes: identification of a new dye for life science applications.

Over-expression and production of plant allergens by molecular farming strategies.

Antibody production in transgenic plants.

Plant-based production of biopharmaceuticals.

Viroid-induced RNA silencing of GFP-viroid fusion transgenes does not induce extensive spreading of methylation or transitive silencing.

Preliminary X-ray analysis of a human V(H) fragment at 1.8 A resolution.

Fusion proteins comprising a Fusarium-specific antibody linked to antifungal peptides protect plants against a fungal pathogen.

Measurement of antibody-membrane interactions by surface plasmon resonance.

Chemolabile cellular microarrays for screening small molecules and peptides.

Unexpected deposition patterns of recombinant proteins in post-endoplasmic reticulum compartments of wheat endosperm.

Plant cell cultures for the production of recombinant proteins.

Recombinant anti-EGFR immunotoxin 425(scFv)-ETA' demonstrates anti-tumor activity against disseminated human pancreatic cancer in nude mice.

Generation of human antibody fragments against Streptococcus mutans using a phage display chain shuffling approach.

Production of Desmodus rotundus salivary plasminogen activator alpha1 (DSPAalpha1) in tobacco is hampered by proteolysis.

Reversible cross-linking of hyperbranched polymers: a strategy for the combinatorial decoration of multivalent scaffolds.

Opportunities for recombinant antigen and antibody expression in transgenic plants--technology assessment.

Transgenic plants in the biopharmaceutical market.

Sowing the seeds of success: pharmaceutical proteins from plants.

Peptide microarrays for the detection of molecular interactions in cellular signal transduction.

Autophagy promotes MHC class II presentation of peptides from intracellular source proteins.

Molecular farming for new drugs and vaccines. Current perspectives on the production of pharmaceuticals in transgenic plants.

Cationic cell-penetrating peptides interfere with TNF signalling by induction of TNF receptor internalization.

A postcolumn alkalinization procedure enhances the sensitivity of fluorescence detection of fluorescein-labeled substances in RP-HPLC.

Production of an active recombinant thrombomodulin derivative in transgenic tobacco plants and suspension cells.

Antigen-specific targeting and elimination of EBV-transformed B cells by allergen toxins.

Break on through to the other side-biophysics and cell biology shed light on cell-penetrating peptides.

One-step analysis of protein complexes in microliters of cell lysate.

Recombinant soluble human Fcgamma receptor I with picomolar affinity for immunoglobulin G.

Plant-derived pharmaceuticals--the road forward.

Endosperm-specific co-expression of recombinant soybean ferritin and Aspergillus phytase in maize results in significant increases in the levels of bioavailable iron.

Benefit assessment of preventive medical check-ups in patients suffering from chronic granulomatous disease (CGD).

Images in cardiovascular medicine. Cardiomyopathy resulting from primary hyperoxaluria type II.

Application of a Scalable Plant Transient Gene Expression Platform for Malaria Vaccine Development.

A CD14 domain with lipopolysaccharide-binding and -neutralizing activity.

Recombinant bovine uteroglobin at 1.6 A resolution: a preliminary X-ray crystallographic analysis.

Towards industrial usefulness - cryo-cell-banking of transgenic BY-2 cell cultures.

Restrictive cardiomyopathy in a patient with primary hyperoxaluria type II.

The intracellular fate of a recombinant protein is tissue dependent.

Constitutive knox1 gene expression in dandelion (Taraxacum officinale, Web.) changes leaf morphology from simple to compound.

Antibody-based metabolic engineering in plants.

The potential of plant virus vectors for vaccine production.

A targeted protease substrate for a quantitative determination of protease activities in the endolysosomal pathway.

Recombinant anti-hCG antibodies retained in the endoplasmic reticulum of transformed plants lack core-xylose and core-alpha(1,3)-fucose residues.

A recombinant multimeric immunoglobulin expressed in rice shows assembly-dependent subcellular localization in endosperm cells.

Immunomodulation of polyamine biosynthesis in tobacco plants has a significant impact on polyamine levels and generates a dwarf phenotype.

A fluorescence-based synthetic LPS sensor.

Multivalence and spot heterogeneity in microarray-based measurement of binding constants.

Endocytosis targets exogenous material selectively to cathepsin S in live human dendritic cells, while cell-penetrating peptides mediate nonselective transport to cysteine cathepsins.

Functional analysis of the broadly neutralizing human anti-HIV-1 antibody 2F5 produced in transgenic BY-2 suspension cultures.

Thoracic actinomycosis imitating a malignant chest wall tumor. Diagnosis: pulmonary actinomycosis.

A comprehensive model for the cellular uptake of cationic cell-penetrating peptides.

Design of protein membrane interaction inhibitors by virtual ligand screening, proof of concept with the C2 domain of factor V.

Spatial and temporal regulation of the forisome gene for1 in the phloem during plant development.

Recombinant antibody 2G12 produced in maize endosperm efficiently neutralizes HIV-1 and contains predominantly single-GlcNAc N-glycans.

Cup-like acute myeloid leukemia: new disease or artificial phenomenon?

Eukaryotic expression and secretion of EGFP-labeled annexin A5.

Depletion of autoreactive B-lymphocytes by a recombinant myelin oligodendrocyte glycoprotein-based immunotoxin.

Biochemical and functional characterization of anti-HIV antibody-ELP fusion proteins from transgenic plants.

Adenoviral p53 gene transfer and gemcitabine in three patients with liver metastases due to advanced pancreatic carcinoma.

Auxin transport inhibitors impair vesicle motility and actin cytoskeleton dynamics in diverse eukaryotes.

Small cleavable adapters enhance the specific cytotoxicity of a humanized immunotoxin directed against CD64-positive cells.

Generation and characterization of a recombinant antibody fragment that binds to the coat protein of grapevine leafroll-associated virus 3.

Plantibody-mediated inhibition of the Potato leafroll virus P1 protein reduces virus accumulation.

Molecular farming of human tissue transglutaminase in tobacco plants.

Evolution of a regulatory framework for pharmaceuticals derived from genetically modified plants.

Engineering Fusarium head blight resistance in wheat by expression of a fusion protein containing a Fusarium-specific antibody and an antifungal peptide.

HPMA as a scaffold for the modular assembly of functional peptide polymers by native chemical ligation.

Phage-display derived single-chain fragment variable (scFv) antibodies recognizing conformational epitopes of Escherichia coli heat-labile enterotoxin B-subunit.

Granzyme B-H22(scFv), a human immunotoxin targeting CD64 in acute myeloid leukemia of monocytic subtypes.

An assay for the detection of grapevine leafroll-associated virus 3 using a single-chain fragment variable antibody.

Grapevine fanleaf virus (GFLV)-specific antibodies confer GFLV and Arabis mosaic virus (ArMV) resistance in Nicotiana benthamiana.

Recombinant-antibody-mediated resistance against Tomato yellow leaf curl virus in Nicotiana benthamiana.

Epstein-Barr virus BDLF2-BMRF2 complex affects cellular morphology.

Methyl jasmonate induced accumulation of kalopanaxsaponin I in Nigella sativa.

Recombinant bispecific single chain antibody fragments induce Fc gamma-receptor-mediated elimination of CD30+ lymphoma cells.

Polyphenoloxidase silencing affects latex coagulation in Taraxacum species.

The structure of the dizinc subclass B2 metallo-beta-lactamase CphA reveals that the second inhibitory zinc ion binds in the histidine site.

Influence of elastin-like peptide fusions on the quantity and quality of a tobacco-derived human immunodeficiency virus-neutralizing antibody.

Genetic determinants of Pseudomonas aeruginosa biofilm establishment.

A cell-penetrating peptide derived from human lactoferrin with conformation-dependent uptake efficiency.

A doubly labeled penetratin analogue as a ratiometric sensor for intracellular proteolytic stability.

Potato virus X as a novel platform for potential biomedical applications.

Characterization and diagnostic potential of foreign epitope-presenting Ty1 virus-like particles expressed in Escherichia coli and Pichia pastoris.

Generation and characterization of a scFv against recombinant coat protein of the geminivirus tomato leaf curl New Delhi virus.

Immunogenic properties of chimeric potato virus X particles displaying the hepatitis C virus hypervariable region I peptide R9.

Characterization of rubber particles and rubber chain elongation in Taraxacum koksaghyz.

The changing fate of a secretory glycoprotein in developing maize endosperm.

Rapid optical imaging of EGF receptor expression with a single-chain antibody SNAP-tag fusion protein.

Emphysematous gastritis caused by Sarcina ventriculi.

Biochemical properties of the matrix metalloproteinase NtMMP1 from Nicotiana tabacum cv. BY-2 suspension cells.

A monoclonal antibody that specifically binds chitosan in vitro and in situ on fungal cell walls.

Rapid transient production in plants by replicating and non-replicating vectors yields high quality functional anti-HIV antibody.

GMP issues for recombinant plant-derived pharmaceutical proteins.

Harmonine, a defence compound from the harlequin ladybird, inhibits mycobacterial growth and demonstrates multi-stage antimalarial activity.

How recombinant swollenin from Kluyveromyces lactis affects cellulosic substrates and accelerates their hydrolysis.

In vivo imaging of immunotoxin treatment using Katushka-transfected A-431 cells in a murine xenograft tumour model.

Generation and comparative characterization of glycosylated and aglycosylated human IgG1 antibodies.

In vivo expression and binding activity of scFv-RWAV, which recognizes the coat protein of tomato leaf curl New Delhi virus (family Geminiviridae).

Development of viral nanoparticles for efficient intracellular delivery.

Proteomic analysis of latex from the rubber-producing plant Taraxacum brevicorniculatum.

Monoclonal tobacco cell lines with enhanced recombinant protein yields can be generated from heterogeneous cell suspension cultures by flow sorting.

Proteins from the FLOWERING LOCUS T-like subclade of the PEBP family act antagonistically to regulate floral initiation in tobacco.

Processing heterogeneous biomass: overcoming the hurdles in model building.

Hyperthermophilic endoglucanase for in planta lignocellulose conversion.

Histone acetylation mediates epigenetic regulation of transcriptional reprogramming in insects during metamorphosis, wounding and infection.

A monoclonal antibody for the detection of SNAP/CLIP-tagged proteins.

With or without sugar? (A)glycosylation of therapeutic antibodies.

Flow cytometry-based ultra-high-throughput screening assay for cellulase activity.

Malaria parasites co-opt human factor H to prevent complement-mediated lysis in the mosquito midgut.

Brain infection and activation of neuronal repair mechanisms by the human pathogen Listeria monocytogenes in the lepidopteran model host Galleria mellonella.

Commercial aspects of pharmaceutical protein production in plants.

Optimizing the yield of recombinant pharmaceutical proteins in plants.

Molecular farming of pharmaceutical proteins using plant suspension cell and tissue cultures.

Rapid high-level production of functional HIV broadly neutralizing monoclonal antibodies in transient plant expression systems.

Yeast surface display for the expression, purification and characterization of wild-type and B11 mutant glucose oxidases.

Changes in the transcriptome of the malaria parasite Plasmodium falciparum during the initial phase of transmission from the human to the mosquito.

Ethanol inducible expression of a mesophilic cellulase avoids adverse effects on plant development.

Galleria mellonella as a model host to study gut microbe homeostasis and brain infection by the human pathogen listeria monocytogenes.

Optimization of BY-2 cell suspension culture medium for the production of a human antibody using a combination of fractional factorial designs and the response surface method.

Cellulases from insects.

Characterization and dynamic behavior of wild yeast during spontaneous wine fermentation in steel tanks and amphorae.

Cellulases for biomass degradation: comparing recombinant cellulase expression platforms.

An efficient process for the saccharification of wood chips by combined ionic liquid pretreatment and enzymatic hydrolysis.

Granzyme M as a novel effector molecule for human cytolytic fusion proteins: CD64-specific cytotoxicity of Gm-H22(scFv) against leukemic cells.

Transcriptome alterations in zebrafish embryos after exposure to environmental estrogens and anti-androgens can reveal endocrine disruption.

Cloning, Heterologous Expression, Purification and Characterization of M12 Mutant of Aspergillus niger Glucose Oxidase in Yeast Pichia pastoris KM71H.

A sensitive monitoring system for mammalian cell cultivation processes: a PAT approach.

Human microtubule-associated protein tau mediates targeted killing of CD30(+) lymphoma cells in vitro and inhibits tumour growth in vivo.

Flocculation increases the efficacy of depth filtration during the downstream processing of recombinant pharmaceutical proteins produced in tobacco.

Genetic engineering and chemical conjugation of potato virus X.

Plant-based production of recombinant Plasmodium surface protein pf38 and evaluation of its potential as a vaccine candidate.

Isolation and characterization of isochorismate synthase and cinnamate 4-hydroxylase during salinity stress, wounding, and salicylic acid treatment in Carthamus tinctorius.

Scale-down models to optimize a filter train for the downstream purification of recombinant pharmaceutical proteins produced in tobacco leaves.

Protective Oral Vaccination against Infectious bursal disease virus Using the Major Viral Antigenic Protein VP2 Produced in Pichia pastoris.

Generic chromatography-based purification strategies accelerate the development of downstream processes for biopharmaceutical proteins produced in plants.

The p38-mediated rapid down-regulation of cell surface gp130 expression impairs interleukin-6 signaling in the synovial fluid of juvenile idiopathic arthritis patients.

Metabolic engineering for p-coumaryl alcohol production in Escherichia coli by introducing an artificial phenylpropanoid pathway.

Plant molecular pharming for the treatment of chronic and infectious diseases.

Perforin-like protein PPLP2 permeabilizes the red blood cell membrane during egress of Plasmodium falciparum gametocytes.

Ultra-high-throughput screening method for the directed evolution of glucose oxidase.

Downstream processing of biopharmaceutical proteins produced in plants: The pros and cons of flocculants.

pH-dependent nano-capturing of tartaric acid using dendrimers.

Inhibition of protease activity by antisense RNA improves recombinant protein production in Nicotiana tabacum cv Bright Yellow 2 (BY-2) suspension cells.

Differential inductions of phenylalanine ammonia-lyase and chalcone synthase during wounding, salicylic acid treatment, and salinity stress in safflower, Carthamus tinctorious.

Alternative intronic polyadenylation generates the interleukin-6 trans-signaling inhibitor sgp130-E10.

EpCAM-selective elimination of carcinoma cells by a novel MAP-based cytolytic fusion protein.

Species-specific considerations in using the fish embryo test as an alternative to identify endocrine disruption.

A CSPG4-specific immunotoxin kills rhabdomyosarcoma cells and binds to primary tumor tissues.

Assessment of cultivation factors that affect biomass and geraniol production in transgenic tobacco cell suspension cultures.

Scaled-up manufacturing of recombinant antibodies produced by plant cells in a 200-L orbitally-shaken disposable bioreactor.

Assessment of the neutrophilic antibody-dependent respiratory burst (ADRB) response to Plasmodium falciparum.

Potato virus X-based expression vectors are stabilized for long-term production of proteins and larger inserts.

Inhibition of the SR protein-phosphorylating CLK kinases of Plasmodium falciparum impairs blood stage replication and malaria transmission.

Malaria vaccine candidate antigen targeting the pre-erythrocytic stage of Plasmodium falciparum produced at high level in plants.

Detailed functional characterization of glycosylated and nonglycosylated variants of malaria vaccine candidate PfAMA1 produced in Nicotiana benthamiana and analysis of growth inhibitory responses in rabbits.

Synthesis and characterization of an insoluble polymer based on polyamidoamine: applications for the decontamination of metals in aqueous systems.

Antimalarial activity of granzyme B and its targeted delivery by a granzyme B-single-chain Fv fusion protein.

Optimization of a multi-stage, multi-subunit malaria vaccine candidate for the production in Pichia pastoris by the identification and removal of protease cleavage sites.

Challenges and advances in the heterologous expression of cellulolytic enzymes: a review.

A high-throughput cellulase screening system based on droplet microfluidics.

In vitro effects and ex vivo binding of an EGFR-specific immunotoxin on rhabdomyosarcoma cells.

Recombinant H22(scFv) blocks CD64 and prevents the capture of anti-TNF monoclonal antibody. A potential strategy to enhance anti-TNF therapy.

The CRISPR/Cas9 system for plant genome editing and beyond.

Yeast surface display is a novel tool for the rapid immunological characterization of plant-derived food allergens.

Comparative evaluation of heterologous production systems for recombinant pulmonary surfactant protein D.

Synthetic polymers are more effective than natural flocculants for the clarification of tobacco leaf extracts.

Cloning murine antibody V-genes with non-degenerate primers and conversion to a recombinant antibody format.

High-value products from plants: the challenges of process optimization.

The increasing value of plant-made proteins.

Cellulose-based filter aids increase the capacity of depth filters during the downstream processing of plant-derived biopharmaceutical proteins.

Heat-precipitation allows the efficient purification of a functional plant-derived malaria transmission-blocking vaccine candidate fusion protein.

Next-generation sequencing is a robust strategy for the high-throughput detection of zygosity in transgenic maize.

Fast track antibody V-gene rescue, recombinant expression in plants and characterization of a PfMSP4-specific antibody.

Angiogenin mutants as novel effector molecules for the generation of fusion proteins with increased cytotoxic potential.

Simple and portable magnetic immunoassay for rapid detection and sensitive quantification of plant viruses.

Pharmacokinetics and immunogenicity of broadly neutralizing HIV monoclonal antibodies in macaques.

The three-dimensional structure of VIM-31--a metallo-β-lactamase from Enterobacter cloacae in its native and oxidized form.

Cell wall modification in tobacco by differential targeting of recombinant endoglucanase from Trichoderma reesei.

Targeted killing of rhabdomyosarcoma cells by a MAP-based human cytolytic fusion protein.

Novel EGFR-specific immunotoxins based on panitumumab and cetuximab show in vitro and ex vivo activity against different tumor entities.

The stage-specific in vitro efficacy of a malaria antigen cocktail provides valuable insights into the development of effective multi-stage vaccines.

Synthesis and evaluation of the antiplasmodial activity of tryptanthrin derivatives.

The Fc-alpha receptor is a new target antigen for immunotherapy of myeloid leukemia.

Thanatin confers partial resistance against aflatoxigenic fungi in maize (Zea mays).

Regulatory approval and a first-in-human phase I clinical trial of a monoclonal antibody produced in transgenic tobacco plants.

Analysis of a Multi-component Multi-stage Malaria Vaccine Candidate--Tackling the Cocktail Challenge.

Perforin-like protein PPLP4 is crucial for mosquito midgut infection by Plasmodium falciparum.

Isolation, production and characterization of fully human monoclonal antibodies directed to Plasmodium falciparum MSP10.

From gene to harvest: insights into upstream process development for the GMP production of a monoclonal antibody in transgenic tobacco plants.

Targeting CD64 mediates elimination of M1 but not M2 macrophages in vitro and in cutaneous inflammation in mice and patient biopsies.

Targeted gene exchange in plant cells mediated by a zinc finger nuclease double cut.

Production of Immunoabsorbent Nanoparticles by Displaying Single-Domain Protein A on Potato Virus X.

The Plasmodium falciparum blood stages acquire factor H family proteins to evade destruction by human complement.

Novel angiogenin mutants with increased cytotoxicity enhance the depletion of pro-inflammatory macrophages and leukemia cells ex vivo.

A WD40-repeat protein unique to malaria parasites associates with adhesion protein complexes and is crucial for blood stage progeny.

Antibody-Mediated Pathogen Resistance in Plants.

Plant expression and characterization of the transmission-blocking vaccine candidate PfGAP50.

Developments in the production of mucosal antibodies in plants.

Overcoming low yields of plant-made antibodies by a protein engineering approach.

Polyclonal antibodies for specific detection of tobacco host cell proteins can be efficiently generated following RuBisCO depletion and the removal of endotoxins.

Production of Hybrid Chimeric PVX Particles Using a Combination of TMV and PVX-Based Expression Vectors.

Comparison of Thermobifida fusca Cellulases Expressed in Escherichia coli and Nicotiana tabacum Indicates Advantages of the Plant System for the Expression of Bacterial Cellulases.

Depth Filters Containing Diatomite Achieve More Efficient Particle Retention than Filters Solely Containing Cellulose Fibers.

Fully human MAP-fusion protein selectively targets and eliminates proliferating CD64(+) M1 macrophages.

Development of a Competitive Cystatin C-Specific Bioassay Suitable for Repetitive Measurements.

Granzyme B-based cytolytic fusion protein targeting EpCAM specifically kills triple negative breast cancer cells in vitro and inhibits tumor growth in a subcutaneous mouse tumor model.

Acquired immune responses to three malaria vaccine candidates and their relationship to invasion inhibition in two populations naturally exposed to malaria.

Novel fusion proteins for the antigen-specific staining and elimination of B cell receptor-positive cell populations demonstrated by a tetanus toxoid fragment C (TTC) model antigen.

A novel fully-human cytolytic fusion protein based on granzyme B shows in vitro cytotoxicity and ex vivo binding to solid tumors overexpressing the epidermal growth factor receptor.

Optimized Blanching Reduces the Host Cell Protein Content and Substantially Enhances the Recovery and Stability of Two Plant-Derived Malaria Vaccine Candidates.

More for less: Improving the biomass yield of a pear cell suspension culture by design of experiments.

Putting the Spotlight Back on Plant Suspension Cultures.

A novel approach for targeted elimination of CSPG4-positive triple-negative breast cancer cells using a MAP tau-based fusion protein.

A Plant-Based Transient Expression System for the Rapid Production of Malaria Vaccine Candidates.

A Monoclonal Antibody That Discriminates Between SNAP-Tagged and CLIP-Tagged Proteins.

Analysis of the dose-dependent stage-specific in vitro efficacy of a multi-stage malaria vaccine candidate cocktail.

Differences in toxicity of anionic and cationic PAMAM and PPI dendrimers in zebrafish embryos and cancer cell lines.

Progress and obstacles in the production and application of recombinant lignin-degrading peroxidases.

Controlled Release of Nor-β-lapachone by PLGA Microparticles: A Strategy for Improving Cytotoxicity against Prostate Cancer Cells.

SNAP-Tag Technology: A Useful Tool To Determine Affinity Constants and Other Functional Parameters of Novel Antibody Fragments.

Photoimmunotheranostic agents for triple-negative breast cancer diagnosis and therapy that can be activated on demand.