measuring-dna-damage-repair-mouse-splenocytes-after-chronic-vivo

Measuring DNA Damage and Repair in Mouse Splenocytes After Chronic <em>In Vivo</em> Exposure to Very Low Doses of Beta- and Gamma-Radiation

Low dose radiation exposure may produce a variety of biological effects that are different in quantity and quality from the effects produced by high ...

Canadian Nuclear Laboratories

Anesthesia monitoring equipment for laboratory animals.

In this article we discuss the importance of monitoring body temperature. Some effects of hypothermia are covered, along with the benefits of careful monitoring. Also discussed are the basic use and application of the most common monitoring methods, as well as the methods we used to help maintain body temperature.

Principles of anesthesia monitoring--body temperature.

Principles of anesthesia monitoring--respiration.

Principles of anesthesia monitoring--arterial blood pressure.

Principles of anesthesia monitoring and electrocardiogram.

Principles of anesthesia monitoring-capnography.

Health effects of tritium, a β-emitter and a by-product of the nuclear industry, is a subject of significant controversy. This mouse in vivo study was undertaken to monitor biological effects of low level tritium exposure. Mice were exposed to tritiated drinking water (HTO) at 10 KBq/L, 1 MBq/L and 20 MBq/L concentrations for one month. The treatment did not result in a significant increase of apoptosis in splenocytes. To examine if this low level tritium exposure alters radiosensitivity, the extracted splenocytes were challenged in vitro with 2 Gy γ-radiation, and apoptotic responses at 1 and 24 h were measured. No alterations in the radiosensitivity were detected in cells from mice exposed to tritium compared to sham-treated mice. In contrast, low dose γ-irradiation at 20 or 100 mGy, resulted in a significant increase in resistance to apoptotic cell death after 2 Gy irradiation; an indication of the radioadaptive response. Overall, our data suggest that low concentrations of tritium given to mice as HTO in drinking water do not exert cytotoxic effect in splenocytes, nor do they change cellular sensitivity to additional high dose γ-radiation. The latter may be considered as the lack of a radioadaptive response, typically observed after low dose γ-irradiation.

The lack of cytotoxic effect and radioadaptive response in splenocytes of mice exposed to low level internal β-particle irradiation through tritiated drinking water in vivo.

Matthew Flegal

Radiological Protection Research and Instrumentation

Canadian Nuclear Laboratories

Measuring DNA Damage and Repair in Mouse Splenocytes After Chronic In Vivo Exposure to Very Low Doses of Beta- and Gamma-Radiation

Matthew Flegal

.css-o250i3{font-size:18px;font-family:Open Sans,sans-serif;line-height:21.6px;}Radiological Protection Research and Instrumentation

Canadian Nuclear Laboratories

Measuring DNA Damage and Repair in Mouse Splenocytes After Chronic In Vivo Exposure to Very Low Doses of Beta- and Gamma-Radiation

Radiological Protection Research and Instrumentation