Michael E. Himmel

Exploration of cellulose surface-binding properties of acidothermus cellulolyticus Cel5A by site-specific mutagenesis.

Effect of single active-site cleft mutation on product specificity in a thermostable bacterial cellulase.

Automated filter paper assay for determination of cellulase activity.

The bacterial scaffoldin: structure, function and potential applications in the nanosciences.

Direct imaging of surface-enhanced Raman scattering in the near field.

Catalytically enhanced endocellulase Cel5A from Acidothermus cellulolyticus.

Acidic sugar degradation pathways: an ab initio molecular dynamics study.

Electrochemical oxidation of water by a cellobiose dehydrogenase from Phanerochaete chrysosporium.

Ab initio molecular dynamics simulations of beta-D-glucose and beta-D-xylose degradation mechanisms in acidic aqueous solution.

Self-assembly of photoactive TiO2-cyclodextrin wires.

Beta-D-glucosidase reaction kinetics from isothermal titration microcalorimetry.

Computer simulation studies of microcrystalline cellulose Ibeta.

The maize primary cell wall microfibril: a new model derived from direct visualization.

Determination of lignin by size exclusion chromatography using multi angle laser light scattering.

Mechanisms of glycerol dehydration.

Outlook for cellulase improvement: screening and selection strategies.

Catalyst transport in corn stover internodes: elucidating transport mechanisms using Direct Blue-I.

Energetics of xylose decomposition as determined using quantum mechanics modeling.

Versatile derivatives of carbohydrate-binding modules for imaging of complex carbohydrates approaching the molecular level of resolution.

Expression and characterization of Acidothermus cellulolyticus E1 endoglucanase in transgenic duckweed Lemna minor 8627.

Biomass recalcitrance: engineering plants and enzymes for biofuels production.

Fractionating recalcitrant lignocellulose at modest reaction conditions.

Cellulase digestibility of pretreated biomass is limited by cellulose accessibility.

Microscopic analysis of corn fiber using starch- and cellulose-specific molecular probes.

Molecular modeling suggests induced fit of Family I carbohydrate-binding modules with a broken-chain cellulose surface.

The potential of cellulases and cellulosomes for cellulosic waste management.

Deposition of lignin droplets produced during dilute acid pretreatment of maize stems retards enzymatic hydrolysis of cellulose.

Synergistic enhancement of cellobiohydrolase performance on pretreated corn stover by addition of xylanase and esterase activities.

Heterologous expression of Aspergillus niger beta-D-xylosidase (XlnD): characterization on lignocellulosic substrates.

Heterologous expression of two ferulic acid esterases from Penicillium funiculosum.

Implications of cellobiohydrolase glycosylation for use in biomass conversion.

Welcome to biotechnology for biofuels.

A biophysical perspective on the cellulosome: new opportunities for biomass conversion.

Heterologous expression of glycosyl hydrolases in planta: a new departure for biofuels.

Visualizing lignin coalescence and migration through maize cell walls following thermochemical pretreatment.

Redistribution of xylan in maize cell walls during dilute acid pretreatment.

Engineered carbohydrate-binding module (CBM) protein-suspended single-walled carbon nanotubes in water.

The effect of lignin removal by alkaline peroxide pretreatment on the susceptibility of corn stover to purified cellulolytic and xylanolytic enzymes.

Detecting cellulase penetration into corn stover cell walls by immuno-electron microscopy.

Perspectives and new directions for the production of bioethanol using consolidated bioprocessing of lignocellulose.

Lignocellulose conversion to biofuels: current challenges, global perspectives.

The effects of water on beta-D-xylose condensation reactions.

The energy landscape for the interaction of the family 1 carbohydrate-binding module and the cellulose surface is altered by hydrolyzed glycosidic bonds.

Computational simulations of the Trichoderma reesei cellobiohydrolase I acting on microcrystalline cellulose Ibeta: the enzyme-substrate complex.

Plant cell wall characterization using scanning probe microscopy techniques.

Identification of amino acids responsible for processivity in a Family 1 carbohydrate-binding module from a fungal cellulase.

Glucose reversion reaction kinetics.

Cellulose crystallinity index: measurement techniques and their impact on interpreting cellulase performance.

Label-free, real-time monitoring of biomass processing with stimulated Raman scattering microscopy.

The unique binding mode of cellulosomal CBM4 from Clostridium thermocellum cellobiohydrolase A.

The role of hydrogen-bonding interactions in acidic sugar reaction pathways.

Structure of a fibronectin type III-like module from Clostridium thermocellum.

Simulation studies of the insolubility of cellulose.

Antisense down-regulation of 4CL expression alters lignification, tree growth, and saccharification potential of field-grown poplar.

Sequencing of multiple clostridial genomes related to biomass conversion and biofuel production.

Free energy landscape for glucose condensation reactions.

Modeling the self-assembly of the cellulosome enzyme complex.

The O-glycosylated linker from the Trichoderma reesei Family 7 cellulase is a flexible, disordered protein.

In situ imaging of single carbohydrate-binding modules on cellulose microfibrils.

Applications of computational science for understanding enzymatic deconstruction of cellulose.

In planta expression of A. cellulolyticus Cel5A endocellulase reduces cell wall recalcitrance in tobacco and maize.

Cellobiohydrolase hydrolyzes crystalline cellulose on hydrophobic faces.

High throughput determination of glucan and xylan fractions in lignocelluloses.

High-temperature behavior of cellulose I.

Sugar-binding sites on the surface of the carbohydrate-binding module of CBH I from Trichoderma reesei.

Molecular-level origins of biomass recalcitrance: decrystallization free energies for four common cellulose polymorphs.

Probing carbohydrate product expulsion from a processive cellulase with multiple absolute binding free energy methods.

Structure of CBM4 from Clostridium thermocellum cellulase K.

Bioprospecting metagenomics of decaying wood: mining for new glycoside hydrolases.

Protein allostery at the solid-liquid interface: endoglucanase attachment to cellulose affects glucan clenching in the binding cleft.

Multiple functions of aromatic-carbohydrate interactions in a processive cellulase examined with molecular simulation.

Effects of alkaline or liquid-ammonia treatment on crystalline cellulose: changes in crystalline structure and effects on enzymatic digestibility.

Elucidating the role of ferrous ion cocatalyst in enhancing dilute acid pretreatment of lignocellulosic biomass.

Weakly hydrated surfaces and the binding interactions of small biological solutes.

A 1.5 Å resolution X-ray structure of the catalytic module of Caldicellulosiruptor bescii family 3 pectate lyase.

Computational investigation of glycosylation effects on a family 1 carbohydrate-binding module.

Harnessing glycosylation to improve cellulase activity.

Molecular dynamics simulations of the interaction of glucose with imidazole in aqueous solution.

Structure and function of the Clostridium thermocellum cellobiohydrolase A X1-module repeat: enhancement through stabilization of the CbhA complex.

Deconstruction of lignocellulosic biomass to fuels and chemicals.

Tracking dynamics of plant biomass composting by changes in substrate structure, microbial community, and enzyme activity.

Binding preferences, surface attachment, diffusivity, and orientation of a family 1 carbohydrate-binding module on cellulose.

The metagenome of an anaerobic microbial community decomposing poplar wood chips.

Product binding varies dramatically between processive and nonprocessive cellulase enzymes.

Challenges for assessing the performance of biomass degrading biocatalysts.

Coherent Raman microscopy analysis of plant cell walls.

Single-molecule tracking of carbohydrate-binding modules on cellulose using fluorescence microscopy.

Analysis of transgenic glycoside hydrolases expressed in plants: T. reesei CBH I and A. cellulolyticus EI.

Improved ethanol yield and reduced Minimum Ethanol Selling Price (MESP) by modifying low severity dilute acid pretreatment with deacetylation and mechanical refining: 1) Experimental.

Considering water availability and the effect of solute concentration on high solids saccharification of lignocellulosic biomass.

Improved ethanol yield and reduced minimum ethanol selling price (MESP) by modifying low severity dilute acid pretreatment with deacetylation and mechanical refining: 2) Techno-economic analysis.

Sequence, structure, and evolution of cellulases in glycoside hydrolase family 48.

Paradigmatic status of an endo- and exoglucanase and its effect on crystalline cellulose degradation.

Cellulase linkers are optimized based on domain type and function: insights from sequence analysis, biophysical measurements, and molecular simulation.

How does plant cell wall nanoscale architecture correlate with enzymatic digestibility?

Computational investigation of the pH dependence of loop flexibility and catalytic function in glycoside hydrolases.

The structure and mode of action of Caldicellulosiruptor bescii family 3 pectate lyase in biomass deconstruction.

Crystal structure and computational characterization of the lytic polysaccharide monooxygenase GH61D from the Basidiomycota fungus Phanerochaete chrysosporium.

Binding site dynamics and aromatic-carbohydrate interactions in processive and non-processive family 7 glycoside hydrolases.

Structural characterization of a unique marine animal family 7 cellobiohydrolase suggests a mechanism of cellulase salt tolerance.

Hydration and saccharification of cellulose Iβ, II and III(I) at increasing dry solids loadings.

Glycosylated linkers in multimodular lignocellulose-degrading enzymes dynamically bind to cellulose.

Endoglucanase peripheral loops facilitate complexation of glucan chains on cellulose via adaptive coupling to the emergent substrate structures.

Improving activity of minicellulosomes by integration of intra- and intermolecular synergies.

3D electron tomography of pretreated biomass informs atomic modeling of cellulose microfibrils.

Genomic, proteomic, and biochemical analyses of oleaginous Mucor circinelloides: evaluating its capability in utilizing cellulolytic substrates for lipid production.

Impact of alg3 gene deletion on growth, development, pigment production, protein secretion, and functions of recombinant Trichoderma reesei cellobiohydrolases in Aspergillus niger.

Cel48A from Thermobifida fusca: structure and site directed mutagenesis of key residues.

Glycoside hydrolase processivity is directly related to oligosaccharide binding free energy.

Structure of Acidothermus cellulolyticus family 74 glycoside hydrolase at 1.82 Å resolution.

Revealing nature's cellulase diversity: the digestion mechanism of Caldicellulosiruptor bescii CelA.

The complete mitochondrial genome of Limnoria quadripunctata Holthuis (Isopoda: Limnoriidae).

Multiscale deconstruction of molecular architecture in corn stover.

Experimental and modeling studies of an unusual water-filled pore structure with possible mechanistic implications in family 48 cellulases.

Charge engineering of cellulases improves ionic liquid tolerance and reduces lignin inhibition.

Effect of mechanical disruption on the effectiveness of three reactors used for dilute acid pretreatment of corn stover Part 2: morphological and structural substrate analysis.

Agave proves to be a low recalcitrant lignocellulosic feedstock for biofuels production on semi-arid lands.

Effect of mechanical disruption on the effectiveness of three reactors used for dilute acid pretreatment of corn stover Part 1: chemical and physical substrate analysis.

Comparison of transcriptional profiles of Clostridium thermocellum grown on cellobiose and pretreated yellow poplar using RNA-Seq.

Response to Comment on "Revealing nature's cellulase diversity: the digestion mechanism of Caldicellulosiruptor bescii CelA".

Specificity of O-glycosylation in enhancing the stability and cellulose binding affinity of Family 1 carbohydrate-binding modules.

Towards a molecular-level theory of carbohydrate processivity in glycoside hydrolases.

Carbohydrate-protein interactions that drive processive polysaccharide translocation in enzymes revealed from a computational study of cellobiohydrolase processivity.

Predicting enzyme adsorption to lignin films by calculating enzyme surface hydrophobicity.

The structure of the catalytic domain of a plant cellulose synthase and its assembly into dimers.

NIR and Py-mbms coupled with multivariate data analysis as a high-throughput biomass characterization technique: a review.

Deletion of Caldicellulosiruptor bescii CelA reveals its crucial role in the deconstruction of lignocellulosic biomass.

Engineering towards a complete heterologous cellulase secretome in Yarrowia lipolytica reveals its potential for consolidated bioprocessing.

Heterologous expression of xylanase enzymes in lipogenic yeast Yarrowia lipolytica.

Heterologous protein expression in Hypocrea jecorina: a historical perspective and new developments.

High temperature pre-digestion of corn stover biomass for improved product yields.

Fungal cellulases.

Discrete and structurally unique proteins (tāpirins) mediate attachment of extremely thermophilic Caldicellulosiruptor species to cellulose.

Homologous expression of the Caldicellulosiruptor bescii CelA reveals that the extracellular protein is glycosylated.

Annual acknowledgement of manuscript reviewers.

The molecular origins of twist in cellulose I-beta.

A constitutive expression system for glycosyl hydrolase family 7 cellobiohydrolases in Hypocrea jecorina.

Cofactor Specificity of the Bifunctional Alcohol and Aldehyde Dehydrogenase (AdhE) in Wild-Type and Mutant Clostridium thermocellum and Thermoanaerobacterium saccharolyticum.

Identifying the ionically bound cell wall and intracellular glycoside hydrolases in late growth stage Arabidopsis stems: implications for the genetic engineering of bioenergy crops.

Expression of the Acidothermus cellulolyticus E1 endoglucanase in Caldicellulosiruptor bescii enhances its ability to deconstruct crystalline cellulose.

In situ micro-spectroscopic investigation of lignin in poplar cell walls pretreated by maleic acid.

The catalytic mechanism and unique low pH optimum of Caldicellulosiruptor bescii family 3 pectate lyase.